FastQCFastQC Report
Thu 26 May 2016
SRR1548083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548083_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1521117
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.30247508902996945No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28450.18703360753972245No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19170.12602580866560562No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17540.11530999916508725No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17450.11471832870186843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG650.045.0000041
GTCGACG207.034006E-445.00000442
CAACGTA207.034006E-445.00000433
ATAGCCG207.034006E-445.00000421
CGCGAAT207.034006E-445.00000415
TAGCCGA207.034006E-445.00000422
ACGATAT207.034006E-445.00000439
CGTAAAC207.034006E-445.00000431
CGCACCG207.034006E-445.00000439
TTCGCGA207.034006E-445.00000442
CAATCGT207.034006E-445.00000440
GCCGTAT207.034006E-445.00000442
AATCGTA207.034006E-445.00000410
TCGCACG351.2122837E-745.01
TCGCAAT253.8914033E-545.044
GCGCGTC850.045.038
TCGATAG551.8189894E-1245.01
CGCATCG1400.043.39285721
TATAGCG800.042.1875041
TATTACG750.042.01