##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1548083_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1521117 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.577841152258504 31.0 31.0 33.0 30.0 34.0 2 31.98424578779936 31.0 31.0 34.0 30.0 34.0 3 32.07983343819049 33.0 31.0 34.0 30.0 34.0 4 35.792861430120105 37.0 35.0 37.0 35.0 37.0 5 35.70322401235408 37.0 35.0 37.0 33.0 37.0 6 35.748863499651904 37.0 35.0 37.0 33.0 37.0 7 36.10151750325583 37.0 35.0 37.0 35.0 37.0 8 36.127929015322295 37.0 35.0 37.0 35.0 37.0 9 37.96570743736346 39.0 38.0 39.0 35.0 39.0 10 37.40553882442968 39.0 37.0 39.0 35.0 39.0 11 37.060238627271936 39.0 37.0 39.0 33.0 39.0 12 36.42926809706288 38.0 35.0 39.0 33.0 39.0 13 36.170284074137626 38.0 35.0 39.0 32.0 39.0 14 37.18659051210393 39.0 35.0 41.0 32.0 41.0 15 37.384726487180146 39.0 35.0 41.0 32.0 41.0 16 37.520389950279956 39.0 35.0 41.0 33.0 41.0 17 37.45208028047809 39.0 35.0 41.0 33.0 41.0 18 37.40875225245658 39.0 35.0 41.0 33.0 41.0 19 37.354338292189226 39.0 35.0 41.0 32.0 41.0 20 37.24161783741816 39.0 35.0 41.0 32.0 41.0 21 37.11443695652603 39.0 35.0 41.0 32.0 41.0 22 37.04370078041334 39.0 35.0 41.0 32.0 41.0 23 36.9647272366294 39.0 35.0 41.0 32.0 41.0 24 36.98183703160243 39.0 35.0 41.0 32.0 41.0 25 36.96070059042138 38.0 35.0 41.0 32.0 41.0 26 36.8390991619974 38.0 35.0 41.0 32.0 41.0 27 36.79318619146325 38.0 35.0 41.0 31.0 41.0 28 36.773511176326345 38.0 35.0 41.0 31.0 41.0 29 36.71207014319083 38.0 35.0 41.0 31.0 41.0 30 36.628041761416114 38.0 35.0 40.0 31.0 41.0 31 36.55479098583475 38.0 35.0 41.0 31.0 41.0 32 36.4290366881706 38.0 35.0 41.0 31.0 41.0 33 36.35274604123154 38.0 35.0 41.0 30.0 41.0 34 36.2548876910849 38.0 35.0 41.0 30.0 41.0 35 36.11484323691077 38.0 35.0 41.0 30.0 41.0 36 35.93715868010153 38.0 35.0 40.0 30.0 41.0 37 35.86779517946351 38.0 35.0 40.0 29.0 41.0 38 35.715374951433716 38.0 35.0 40.0 29.0 41.0 39 35.65489834115324 38.0 35.0 40.0 29.0 41.0 40 35.488191243671594 38.0 35.0 40.0 28.0 41.0 41 35.386029477022475 38.0 34.0 40.0 27.0 41.0 42 35.363319192409264 38.0 34.0 40.0 27.0 41.0 43 35.28647829193941 38.0 34.0 40.0 27.0 41.0 44 35.11056743169657 37.0 34.0 40.0 27.0 41.0 45 35.05021441480175 37.0 34.0 40.0 27.0 41.0 46 34.97514326642855 37.0 34.0 40.0 26.0 41.0 47 34.85354643988595 37.0 34.0 40.0 26.0 41.0 48 34.7327740075221 37.0 34.0 40.0 26.0 41.0 49 34.65642945282973 36.0 34.0 40.0 26.0 41.0 50 34.47146340485315 36.0 34.0 40.0 24.0 41.0 51 34.08947240744795 36.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 7.0 13 6.0 14 19.0 15 31.0 16 64.0 17 193.0 18 407.0 19 895.0 20 1562.0 21 2720.0 22 4322.0 23 6497.0 24 9626.0 25 13543.0 26 18088.0 27 21601.0 28 24845.0 29 28657.0 30 34825.0 31 43084.0 32 54065.0 33 70627.0 34 123619.0 35 197402.0 36 103048.0 37 135779.0 38 219032.0 39 406476.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.223731639315055 23.70619748513757 29.909796550824165 12.160274324723213 2 30.468464950427876 25.043767178987547 31.670279143550427 12.817488727034146 3 30.562014624779028 25.391340705547304 30.351314198710554 13.695330470963116 4 26.985300933458767 27.324459591208306 30.134105397546673 15.556134077786258 5 23.64459801580023 32.33663156745997 28.517595950870316 15.501174465869488 6 21.254578050209155 41.741167839160305 26.446157659141278 10.558096451489266 7 90.29055621625423 3.3740336870865293 4.874312758321681 1.4610973383375505 8 90.88998413665746 2.5400412985983327 4.559609813051856 2.0103647516923417 9 85.84467861446555 5.022164632963802 6.453678448140413 2.6794783044302313 10 45.17634080744611 31.109112579768684 12.182297614187469 11.53224899859774 11 36.61283122863001 23.109004764262053 24.435924389774094 15.842239617333842 12 34.202628726126925 22.515493548491012 26.056444047367822 17.225433678014248 13 23.563078974201197 32.427748818795656 27.129931491134474 16.87924071586867 14 17.882122151024543 37.01694215500846 27.57243525645956 17.52850043750744 15 16.37783286887202 26.959070209589402 40.04991069063063 16.613186230907946 16 20.808918709080235 23.36217398135712 38.22907771065605 17.599829598906595 17 19.896036925496198 22.983636367222246 27.69136101956654 29.428965687715014 18 23.04990345910275 26.682168432803 30.675812577204773 19.59211553088947 19 27.83737214165643 28.273367531886105 25.09787215579078 18.791388170666686 20 29.286241623754123 27.447264082907495 24.316801403179376 18.94969289015901 21 24.399963973842905 28.107831284510006 28.738420515976088 18.753784225671 22 23.706460449787887 22.626135925112926 28.358568078589613 25.308835546509574 23 19.996752386568556 29.699490571731168 27.750462324725845 22.553294716974435 24 20.517488135363685 26.57750850197585 34.2742208521764 18.630782510484075 25 21.364102827067214 26.45647902166631 31.577321139662494 20.602097011603973 26 19.122131959606 33.679592036641495 25.71228906126222 21.485986942490285 27 19.30929704947088 31.566210883186503 30.127728504776425 18.996763562566194 28 16.756633447657215 28.96595067966501 35.33699248644253 18.940423386235246 29 18.30082761549572 27.25115819493175 34.369545537917205 20.078468651655328 30 20.94592329189668 28.66952377759239 31.64911048919971 18.73544244131122 31 28.052608707942913 26.499539483156127 25.842127857357454 19.605723951543506 32 28.07824776134906 27.397760987484855 26.39507677581672 18.128914475349365 33 26.295939102646276 26.295347432183057 26.479554169731852 20.92915929543881 34 20.690847581086793 26.95953039772746 29.02012139763082 23.329500623554928 35 21.098311306756813 25.652793309127436 30.10498206252379 23.14391332159196 36 27.30500020708466 25.1066157304139 28.10270347382877 19.485680588672665 37 20.893987773458583 30.296551810281525 28.26975176794421 20.53970864831568 38 21.428397684070326 30.835695084599013 25.902018056467714 21.833889174862946 39 20.68289290041463 28.794234763006394 27.72390289504358 22.798969441535398 40 24.280709504923024 25.424737216137878 28.518187621333535 21.776365657605563 41 19.493175081206772 24.159680024613493 29.769504909878723 26.577639984301012 42 22.22748151522861 27.20974126250643 26.12113335134641 24.441643870918544 43 22.13209108832522 26.604002190495535 26.683811961867498 24.580094759311745 44 21.1045567172019 28.68306645708384 29.287293482355402 20.92508334335886 45 20.116795749439394 32.16360082754976 25.582318782841817 22.13728464016903 46 23.074490653907624 29.18881322081076 27.22091726014501 20.515778865136607 47 22.203748955537282 26.623461574619178 28.30235938458383 22.870430085259713 48 21.882537635172046 26.12665560900312 30.875008299821776 21.115798456003056 49 22.0044874917577 24.93884428351008 30.73806945816791 22.318598766564307 50 20.56324398451927 28.87207229950096 29.09585521692283 21.468828499056944 51 19.85567185167216 31.296343410796148 26.543125873946583 22.304858863585114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 518.0 1 1246.0 2 1974.0 3 4899.0 4 7824.0 5 5814.5 6 3805.0 7 3757.0 8 3709.0 9 3888.0 10 4067.0 11 4099.0 12 4131.0 13 3994.5 14 3858.0 15 3829.0 16 3800.0 17 4109.5 18 4419.0 19 3795.0 20 3171.0 21 3987.0 22 4803.0 23 6219.5 24 7636.0 25 8577.0 26 12594.0 27 15670.0 28 16635.0 29 17600.0 30 21344.5 31 25089.0 32 30908.5 33 36728.0 34 43740.5 35 50753.0 36 50200.5 37 49648.0 38 58183.0 39 66718.0 40 86729.5 41 106741.0 42 118871.5 43 131002.0 44 135754.0 45 140506.0 46 139343.5 47 138181.0 48 140477.5 49 142774.0 50 132491.0 51 122208.0 52 110194.0 53 98180.0 54 88422.0 55 78664.0 56 70769.0 57 62874.0 58 58022.5 59 53171.0 60 45817.5 61 38464.0 62 33581.5 63 28699.0 64 24127.5 65 19556.0 66 15377.5 67 11199.0 68 9288.0 69 7377.0 70 6905.0 71 6433.0 72 5783.0 73 5133.0 74 3819.5 75 1764.0 76 1022.0 77 702.5 78 383.0 79 341.0 80 299.0 81 237.5 82 176.0 83 115.0 84 54.0 85 41.5 86 29.0 87 28.0 88 27.0 89 20.0 90 13.0 91 10.0 92 7.0 93 3.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1521117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.41693033093571 #Duplication Level Percentage of deduplicated Percentage of total 1 81.12003574513906 16.562221182515184 2 7.6480644585996975 3.1229999843547107 3 2.4421617671950444 1.4958433997308815 4 1.196329459467776 0.9770150090719824 5 0.7217936087978385 0.7368404912070066 6 0.46847862499902704 0.5738937268882615 7 0.35636574428782974 0.5093126201419668 8 0.2914204551899867 0.4759928904498824 9 0.23980366923773902 0.4406449327136702 >10 2.8699998371567803 14.219997774254772 >50 1.3734258999763655 20.740431547439936 >100 1.2637324116726867 38.356154603087475 >500 0.006129924897058981 0.8051370485982609 >1k 0.0022583933831269933 0.9835147895459898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4601 0.30247508902996945 No Hit AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 2845 0.18703360753972245 No Hit GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 1917 0.12602580866560562 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1754 0.11530999916508725 No Hit TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 1745 0.11471832870186843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.059692975622519504 0.0 2 0.0 0.0 0.0 0.22128475324383332 0.0 3 0.0 0.0 0.0 0.31411127480660594 0.0 4 0.0 0.0 0.0 0.481028086596889 0.0 5 0.0 0.0 0.0 0.8126265106497397 0.0 6 0.0 0.0 0.0 1.1119460238758754 0.0 7 0.0 0.0 0.0 1.2945092323601668 0.0 8 0.0 0.0 0.0 1.7459537957961155 0.0 9 0.0 0.0 0.0 1.883024119775139 0.0 10 0.0 0.0 0.0 2.156901803082866 0.0 11 0.0 0.0 0.0 2.569098892458634 0.0 12 0.0 0.0 0.0 2.8799888502988265 0.0 13 0.0 0.0 0.0 3.0085785643050467 0.0 14 0.0 0.0 0.0 3.058673330190906 0.0 15 0.0 0.0 0.0 3.1420331243421775 0.0 16 0.0 0.0 0.0 3.3431353406739914 0.0 17 0.0 0.0 0.0 3.5993943924103142 0.0 18 0.0 0.0 0.0 3.919093666036209 0.0 19 0.0 0.0 0.0 4.088968830142586 0.0 20 0.0 0.0 0.0 4.271071850488818 0.0 21 0.0 0.0 0.0 4.497484414413881 0.0 22 0.0 0.0 0.0 4.749207325932193 0.0 23 0.0 0.0 0.0 5.016839598794833 0.0 24 0.0 0.0 0.0 5.212814004445417 0.0 25 3.287058128993365E-4 0.0 0.0 5.369409453710661 0.0 26 3.287058128993365E-4 0.0 0.0 5.5229808094972315 0.0 27 3.287058128993365E-4 0.0 0.0 5.693710608717146 0.0 28 3.287058128993365E-4 0.0 0.0 5.87548492325048 0.0 29 3.287058128993365E-4 0.0 0.0 6.079019562597749 0.0 30 3.287058128993365E-4 0.0 0.0 6.332517485505718 0.0 31 3.9444697547920377E-4 0.0 0.0 6.556892073390804 0.0 32 3.9444697547920377E-4 0.0 0.0 6.740046952338314 0.0 33 3.9444697547920377E-4 0.0 0.0 6.944107520986223 0.0 34 3.9444697547920377E-4 0.0 0.0 7.15355886496568 0.0 35 3.9444697547920377E-4 0.0 0.0 7.40613641159753 0.0 36 3.9444697547920377E-4 0.0 0.0 7.6153247909266675 0.0 37 3.9444697547920377E-4 0.0 0.0 7.842854954615588 0.0 38 3.9444697547920377E-4 0.0 0.0 8.077024975725076 0.0 39 3.9444697547920377E-4 0.0 0.0 8.410332670005003 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGCG 65 0.0 45.000004 1 GTCGACG 20 7.034006E-4 45.000004 42 CAACGTA 20 7.034006E-4 45.000004 33 ATAGCCG 20 7.034006E-4 45.000004 21 CGCGAAT 20 7.034006E-4 45.000004 15 TAGCCGA 20 7.034006E-4 45.000004 22 ACGATAT 20 7.034006E-4 45.000004 39 CGTAAAC 20 7.034006E-4 45.000004 31 CGCACCG 20 7.034006E-4 45.000004 39 TTCGCGA 20 7.034006E-4 45.000004 42 CAATCGT 20 7.034006E-4 45.000004 40 GCCGTAT 20 7.034006E-4 45.000004 42 AATCGTA 20 7.034006E-4 45.000004 10 TCGCACG 35 1.2122837E-7 45.0 1 TCGCAAT 25 3.8914033E-5 45.0 44 GCGCGTC 85 0.0 45.0 38 TCGATAG 55 1.8189894E-12 45.0 1 CGCATCG 140 0.0 43.392857 21 TATAGCG 80 0.0 42.187504 1 TATTACG 75 0.0 42.0 1 >>END_MODULE