FastQCFastQC Report
Thu 26 May 2016
SRR1548082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548082_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2224347
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173870.7816676085161173No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22770.10236712167660891No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.03471E-445.00000440
GTCGAGT207.03471E-445.00000444
AACGCGT207.03471E-445.00000418
CGTTTTT85950.042.931941
CGGTCTA4150.040.6626531
ACGGTCT4150.040.12048330
TTTAGCG1400.038.5714261
TAAGTCG356.250926E-638.5714261
TTACGAG700.038.5714261
TGCGTAG1650.038.181821
CGAGGGC3250.038.0769234
TAGTACG301.14026545E-437.4999961
CACGACG4450.037.4157326
ACGGGAT9950.037.3115545
TAGGGAT35100.037.179495
TACGCGG1150.037.1739162
TCACGAC4550.037.0879125
TAGGGAC24950.036.9739465
TACGGGA6950.036.906484
AAGGGAT35300.036.9050985
Produced by FastQC (version 0.11.5)