FastQCFastQC Report
Thu 26 May 2016
SRR1548081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548081_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2652475
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139710.5267156146617782No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.0349575E-445.030
CGCTACG207.0349575E-445.033
CGACGGT6600.042.27272828
TAATGCG700.041.7857131
TATACGG3350.041.6417922
CGTTTTT72400.041.519341
AATACGG1050.040.7142872
ATACGCG451.9299478E-840.01
TGCACGG3450.039.7826082
CGGTCTA7150.039.33566331
CGTAAGG3400.039.0441172
GGACCGA11000.038.8636368
CACGACG7200.038.7526
TACGGGA10600.038.6320764
ACGGGAT15800.038.3069655
GTAATCG659.094947E-1238.076921
GACCGAT9400.038.058519
GACGGTC7350.037.95918329
TAAGGGA39550.037.718084
ACGGGAC7000.037.6071435
Produced by FastQC (version 0.11.5)