##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548081_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2652475
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.523971385215695	31.0	31.0	33.0	30.0	34.0
2	31.94377025231152	31.0	31.0	34.0	30.0	34.0
3	31.993528308466622	33.0	31.0	34.0	30.0	34.0
4	35.71480409805936	37.0	35.0	37.0	33.0	37.0
5	35.63368325808914	37.0	35.0	37.0	33.0	37.0
6	35.69512662701816	37.0	35.0	37.0	33.0	37.0
7	36.0791012167881	37.0	35.0	37.0	35.0	37.0
8	36.11242858085373	37.0	35.0	37.0	35.0	37.0
9	37.94985211924712	39.0	38.0	39.0	35.0	39.0
10	37.3294590900951	39.0	37.0	39.0	34.0	39.0
11	37.02073723597772	39.0	37.0	39.0	33.0	39.0
12	36.544915597696495	39.0	35.0	39.0	32.0	39.0
13	36.36298702155534	39.0	35.0	39.0	32.0	39.0
14	37.42911054769602	40.0	35.0	41.0	32.0	41.0
15	37.59975079878227	40.0	35.0	41.0	33.0	41.0
16	37.695937190736956	40.0	35.0	41.0	33.0	41.0
17	37.61829574265544	40.0	35.0	41.0	33.0	41.0
18	37.53431530928661	39.0	36.0	41.0	33.0	41.0
19	37.47702353462332	39.0	36.0	41.0	32.0	41.0
20	37.37014297966993	39.0	35.0	41.0	32.0	41.0
21	37.25790365601938	39.0	35.0	41.0	32.0	41.0
22	37.16827981413586	39.0	35.0	41.0	32.0	41.0
23	37.06036249163517	39.0	35.0	41.0	32.0	41.0
24	37.079033732645925	39.0	35.0	41.0	32.0	41.0
25	37.049967294696465	39.0	35.0	41.0	32.0	41.0
26	36.93171735831629	39.0	35.0	41.0	32.0	41.0
27	36.87010848358609	39.0	35.0	41.0	31.0	41.0
28	36.82654464226807	39.0	35.0	41.0	31.0	41.0
29	36.77665538789244	39.0	35.0	41.0	31.0	41.0
30	36.679613568459644	38.0	35.0	41.0	31.0	41.0
31	36.609916775841434	39.0	35.0	41.0	31.0	41.0
32	36.49820450711128	39.0	35.0	41.0	30.0	41.0
33	36.391828009689064	39.0	35.0	41.0	30.0	41.0
34	36.25845860941197	39.0	35.0	41.0	30.0	41.0
35	36.124226241529136	38.0	35.0	41.0	30.0	41.0
36	35.98468486979142	38.0	35.0	41.0	29.0	41.0
37	35.890593879301406	38.0	35.0	41.0	29.0	41.0
38	35.79174544529166	38.0	35.0	40.0	29.0	41.0
39	35.752157890272294	38.0	35.0	40.0	28.0	41.0
40	35.628156344546134	38.0	35.0	40.0	27.0	41.0
41	35.56989038539477	38.0	35.0	40.0	27.0	41.0
42	35.52856068388957	38.0	35.0	40.0	27.0	41.0
43	35.399134393349605	38.0	34.0	40.0	27.0	41.0
44	35.24058473689667	38.0	34.0	40.0	26.0	41.0
45	35.19283499373227	38.0	34.0	40.0	26.0	41.0
46	35.12066730129408	38.0	34.0	40.0	26.0	41.0
47	35.0312470428562	38.0	34.0	40.0	26.0	41.0
48	34.912451955249345	37.0	34.0	40.0	26.0	41.0
49	34.815965089209136	37.0	34.0	40.0	25.0	41.0
50	34.65364536894787	37.0	34.0	40.0	24.0	41.0
51	34.288478496498556	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	11.0
14	19.0
15	45.0
16	161.0
17	425.0
18	912.0
19	1858.0
20	3129.0
21	5293.0
22	8025.0
23	12057.0
24	18045.0
25	26053.0
26	34812.0
27	40137.0
28	44381.0
29	50276.0
30	59739.0
31	72521.0
32	90512.0
33	116298.0
34	190569.0
35	287972.0
36	187010.0
37	245141.0
38	395021.0
39	761912.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.03903901073526	25.191076258965683	31.037766614199946	13.732118116099116
2	30.05785162913882	25.492040452784664	31.038445225685447	13.411662692391069
3	29.093846313348852	25.48604605132942	32.367694323226424	13.052413312095307
4	25.51575415413906	27.308532596914205	32.555933609176336	14.619779639770403
5	23.577752853467047	31.796152649883595	30.630750525452644	13.995343971196714
6	21.345611174469127	40.95031998416573	27.07086777443708	10.633201066928057
7	90.08985947087154	3.0837236920234874	5.410908679629403	1.4155081574755652
8	91.44414179209983	2.177626556329466	5.092413689101688	1.2858179624690147
9	86.50320926681684	5.017577922506338	6.389730346186109	2.0894824644907115
10	39.39931573341879	36.86409862486922	12.90149765784786	10.835087983864126
11	32.43657338900461	23.507629666632106	27.742089934872148	16.313707009491136
12	31.1992761477488	22.56503831327345	28.561513303612664	17.674172235365084
13	23.197918924777802	29.759413378071425	29.485178936653504	17.55748876049727
14	18.485339164365357	33.35982431502653	29.67251340728942	18.482323113318692
15	16.888227033242536	28.670543548949567	37.787877359824314	16.653352057983582
16	21.060518949283217	25.30779743447158	36.3307099972667	17.300973618978503
17	21.754512295120595	24.798235610137702	28.293084760459568	25.154167334282135
18	22.658837500824703	27.210473237259542	30.78106296949076	19.349626292424997
19	25.649742221887106	28.042714822948376	27.598789809517527	18.708753145646988
20	27.156297420333843	27.685124270728284	26.47252094741703	18.686057361520845
21	24.728376327769347	27.044062620759856	29.684954617856906	18.54260643361389
22	23.69319220727811	23.8142112555255	29.531362218305546	22.961234318890845
23	20.447393472134515	28.16339456545302	29.91157315337562	21.477638809036844
24	21.10915277241067	27.170208955786578	32.88626660006221	18.834371671740545
25	20.651467026079416	28.12535462162697	31.099143252999557	20.124035099294055
26	19.812250822345167	32.24965362538761	27.17311190491899	20.76498364734823
27	19.58842213404462	31.100802081075223	30.9430626113347	18.367713173545464
28	17.847519769272093	29.03231887199691	34.41966465282425	18.700496705906748
29	19.091716227297148	27.20749488685096	33.40902364772524	20.291765238126654
30	20.617724954994863	27.673663276750958	32.271859301218676	19.436752467035504
31	24.91989556923251	27.105439259559468	28.316760761175885	19.65790441003214
32	25.24551598035797	27.947181406045296	28.08018925720318	18.727113356393556
33	24.141301991536206	27.678300455235206	27.706085825502598	20.47431172772599
34	20.12177306100906	27.959773419165117	30.07266797990556	21.84578553992026
35	20.49384065825314	27.176580363622655	30.899744578176985	21.42983439994722
36	25.364197589044196	26.787095071584087	28.659874268371993	19.188833070999728
37	20.8205920885211	29.080274083638862	30.214610882289183	19.884522945550852
38	20.801251661184367	30.713654228597818	27.060236194497595	21.424857915720224
39	21.034354706453406	28.825078464453014	28.576744361398315	21.563822467695264
40	22.353009924692977	27.100952883627556	28.32290596518346	22.223131226496008
41	19.586537102140454	26.600703116900252	29.685256222961577	24.12750355799772
42	22.342868453048567	27.776020509147116	27.083610590109235	22.797500447695075
43	21.900112159398297	27.617640128559174	28.017304592880233	22.464943119162292
44	21.27594039529119	28.761138182263736	29.123026607225327	20.839894815219747
45	20.53267231547894	30.919386610618382	26.683267514302678	21.864673559599996
46	22.22724059604709	29.720619421483708	27.89850988227976	20.153630100189446
47	21.513265912025563	28.463152338853337	28.358872373914927	21.664709375206176
48	22.001112168823457	27.293226137852383	29.735812778631278	20.969848914692882
49	21.599374169407817	26.68496404301643	30.152065523708988	21.563596263866767
50	20.437138898575856	29.223197202612656	29.401709723937074	20.93795417487441
51	19.84161961941206	30.513501541013582	27.57462370050613	22.07025513906823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1268.0
1	2337.5
2	3407.0
3	12942.0
4	22477.0
5	16407.5
6	10338.0
7	9848.5
8	9359.0
9	9895.5
10	10432.0
11	10746.5
12	11061.0
13	10783.0
14	10505.0
15	10170.0
16	9835.0
17	9831.5
18	9828.0
19	9441.0
20	9054.0
21	10123.0
22	11192.0
23	12776.5
24	14361.0
25	17352.0
26	27358.0
27	34373.0
28	40592.0
29	46811.0
30	50352.0
31	53893.0
32	63453.0
33	73013.0
34	83420.0
35	93827.0
36	100530.5
37	107234.0
38	118078.0
39	128922.0
40	152170.0
41	175418.0
42	193619.0
43	211820.0
44	217882.0
45	223944.0
46	229048.5
47	234153.0
48	235352.5
49	236552.0
50	218100.5
51	199649.0
52	180047.5
53	160446.0
54	144142.5
55	127839.0
56	118866.5
57	109894.0
58	97060.5
59	84227.0
60	76125.5
61	68024.0
62	57222.5
63	46421.0
64	39128.5
65	31836.0
66	25129.5
67	18423.0
68	15706.5
69	12990.0
70	10371.0
71	7752.0
72	6664.5
73	5577.0
74	4323.5
75	2160.5
76	1251.0
77	936.5
78	622.0
79	551.5
80	481.0
81	324.0
82	167.0
83	190.5
84	214.0
85	149.0
86	84.0
87	67.5
88	51.0
89	34.5
90	18.0
91	16.5
92	15.0
93	9.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2652475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.952572639519783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85005253177768	16.730640259415424
2	7.784853744489108	3.2622542713889118
3	2.583715740074931	1.6240647517137181
4	1.303885896111998	1.0927905580772792
5	0.7940612668631651	0.8318813187089784
6	0.5348466645550857	0.672384815405718
7	0.4080910027813373	0.5985389465517308
8	0.3271806960822529	0.5484221840729638
9	0.2748750794640389	0.5183406062337642
>10	3.5131463435846673	17.914125366637975
>50	1.5863735246122352	24.191636024966474
>100	1.0343973421987271	30.66800544984578
>500	0.0034353272276011456	0.4718787986611754
>1k	9.040334809476699E-4	0.3457641133092925
>5k	0.0	0.0
>10k+	1.80806696189534E-4	0.5292725350108608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13971	0.5267156146617782	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1310191424989867E-4	0.0	0.0	0.03355356789413661	0.0
2	1.1310191424989867E-4	0.0	0.0	0.1111791817076504	0.0
3	1.1310191424989867E-4	0.0	0.0	0.18409221576075174	0.0
4	1.1310191424989867E-4	0.0	0.0	0.29485669044948587	0.0
5	1.1310191424989867E-4	0.0	0.0	0.5394207296958501	0.0
6	1.1310191424989867E-4	0.0	0.0	0.8142583813231039	0.0
7	1.1310191424989867E-4	0.0	0.0	0.9854569788593672	0.0
8	1.1310191424989867E-4	0.0	0.0	1.3683823598714409	0.0
9	1.1310191424989867E-4	0.0	0.0	1.5102121603408138	0.0
10	1.1310191424989867E-4	0.0	0.0	1.7671797095165835	0.0
11	1.1310191424989867E-4	0.0	0.0	2.1470136382058267	0.0
12	1.1310191424989867E-4	0.0	0.0	2.4451125835304763	0.0
13	1.1310191424989867E-4	0.0	0.0	2.5704672051574473	0.0
14	1.1310191424989867E-4	0.0	0.0	2.622418684436234	0.0
15	1.1310191424989867E-4	0.0	0.0	2.6948039095561693	0.0
16	1.1310191424989867E-4	0.0	0.0	2.8609506215892706	0.0
17	1.1310191424989867E-4	0.0	0.0	3.0681533284950846	0.0
18	1.1310191424989867E-4	0.0	0.0	3.3185986672824437	0.0
19	1.1310191424989867E-4	0.0	0.0	3.470909245138974	0.0
20	1.1310191424989867E-4	0.0	0.0	3.632871186344829	0.0
21	1.1310191424989867E-4	0.0	0.0	3.8402246958029767	0.0
22	1.5080255233319823E-4	0.0	0.0	4.072045919377185	0.0
23	1.885031904164978E-4	0.0	0.0	4.3253942072969584	0.0
24	1.885031904164978E-4	0.0	0.0	4.4985909386516365	0.0
25	1.885031904164978E-4	0.0	0.0	4.6463774399381705	0.0
26	1.885031904164978E-4	0.0	0.0	4.794654049519788	0.0
27	1.885031904164978E-4	0.0	0.0	4.954467054354895	0.0
28	1.885031904164978E-4	0.0	0.0	5.121141575321162	0.0
29	1.885031904164978E-4	0.0	0.0	5.308099039576245	0.0
30	1.885031904164978E-4	0.0	0.0	5.511116975654813	0.0
31	2.639044665830969E-4	0.0	0.0	5.712513784295799	0.0
32	2.639044665830969E-4	0.0	0.0	5.894230859857303	0.0
33	2.639044665830969E-4	0.0	0.0	6.085071489834966	0.0
34	2.639044665830969E-4	0.0	0.0	6.28484717103837	0.0
35	2.639044665830969E-4	0.0	0.0	6.5125967256995825	0.0
36	2.639044665830969E-4	0.0	0.0	6.714936050292652	0.0
37	2.639044665830969E-4	0.0	0.0	6.926361228663795	0.0
38	2.639044665830969E-4	0.0	0.0	7.1512078341925935	0.0
39	2.639044665830969E-4	0.0	0.0	7.444481097842581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.0349575E-4	45.0	30
CGCTACG	20	7.0349575E-4	45.0	33
CGACGGT	660	0.0	42.272728	28
TAATGCG	70	0.0	41.785713	1
TATACGG	335	0.0	41.641792	2
CGTTTTT	7240	0.0	41.51934	1
AATACGG	105	0.0	40.714287	2
ATACGCG	45	1.9299478E-8	40.0	1
TGCACGG	345	0.0	39.782608	2
CGGTCTA	715	0.0	39.335663	31
CGTAAGG	340	0.0	39.044117	2
GGACCGA	1100	0.0	38.863636	8
CACGACG	720	0.0	38.75	26
TACGGGA	1060	0.0	38.632076	4
ACGGGAT	1580	0.0	38.306965	5
GTAATCG	65	9.094947E-12	38.07692	1
GACCGAT	940	0.0	38.05851	9
GACGGTC	735	0.0	37.959183	29
TAAGGGA	3955	0.0	37.71808	4
ACGGGAC	700	0.0	37.607143	5
>>END_MODULE