FastQCFastQC Report
Thu 26 May 2016
SRR1548080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548080_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2100963
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128820.6131473995496352No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22920.10909283028782514No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT207.0346217E-445.038
TACGCGG1250.043.22
TTACGAG950.042.631581
CGTTTTT60850.042.2267881
CTACGCG700.041.7857131
CGGTCTA3200.041.48437531
TATCGCG603.6379788E-1241.2500041
CGGTAGT1850.040.13513612
TCTCACG3500.039.85714323
TAATACG403.4600453E-739.3751
CGCGTGG403.4600453E-739.3752
AGTACCG403.4600453E-739.3751
TAGGGAC24550.038.859475
AGGGCGA12300.038.780496
CACGACG3500.038.57142626
GGGCGAT25900.038.3108147
CTCACGA3650.038.21917724
ATCCGTT659.094947E-1238.07692711
TCGCGTA659.094947E-1238.07692730
CGTAAGG2900.038.0172422
Produced by FastQC (version 0.11.5)