##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548080_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2100963
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59104753391659	31.0	31.0	33.0	30.0	34.0
2	32.004670239314066	31.0	31.0	34.0	30.0	34.0
3	32.05661308647511	33.0	31.0	34.0	30.0	34.0
4	35.771195399443016	37.0	35.0	37.0	35.0	37.0
5	35.69989666643344	37.0	35.0	37.0	33.0	37.0
6	35.77162758220873	37.0	35.0	37.0	35.0	37.0
7	36.11224567019981	37.0	35.0	37.0	35.0	37.0
8	36.15141913493955	37.0	35.0	37.0	35.0	37.0
9	37.997677255620395	39.0	39.0	39.0	35.0	39.0
10	37.46141840670207	39.0	37.0	39.0	35.0	39.0
11	37.07790332338075	39.0	37.0	39.0	33.0	39.0
12	36.50053094699906	39.0	35.0	39.0	33.0	39.0
13	36.28702123740399	39.0	35.0	39.0	32.0	39.0
14	37.362085862530655	40.0	35.0	41.0	32.0	41.0
15	37.56519700727714	40.0	35.0	41.0	33.0	41.0
16	37.67713186762451	40.0	35.0	41.0	33.0	41.0
17	37.617431625402254	39.0	35.0	41.0	33.0	41.0
18	37.53031871575082	39.0	36.0	41.0	33.0	41.0
19	37.463650716361975	39.0	36.0	41.0	33.0	41.0
20	37.338631379990986	39.0	35.0	41.0	32.0	41.0
21	37.22334615126492	39.0	35.0	41.0	32.0	41.0
22	37.144327149026424	39.0	35.0	41.0	32.0	41.0
23	37.06865185155569	39.0	35.0	41.0	32.0	41.0
24	37.078127982263375	39.0	35.0	41.0	32.0	41.0
25	37.06038659414754	39.0	35.0	41.0	32.0	41.0
26	36.94781012326252	39.0	35.0	41.0	32.0	41.0
27	36.89218277523212	39.0	35.0	41.0	32.0	41.0
28	36.8552587551518	38.0	35.0	41.0	32.0	41.0
29	36.82380889144645	38.0	35.0	41.0	31.0	41.0
30	36.74821212939019	38.0	35.0	41.0	31.0	41.0
31	36.65325757759656	38.0	35.0	41.0	31.0	41.0
32	36.53034346630569	38.0	35.0	41.0	31.0	41.0
33	36.425410157151745	38.0	35.0	41.0	30.0	41.0
34	36.29940651025268	38.0	35.0	41.0	30.0	41.0
35	36.17417346235988	38.0	35.0	41.0	30.0	41.0
36	36.015552867899146	38.0	35.0	41.0	30.0	41.0
37	35.93774140715472	38.0	35.0	41.0	29.0	41.0
38	35.85264090800266	38.0	35.0	41.0	29.0	41.0
39	35.81389486630655	38.0	35.0	40.0	29.0	41.0
40	35.686956409989136	38.0	35.0	40.0	29.0	41.0
41	35.62211376402155	38.0	35.0	40.0	28.0	41.0
42	35.59624895821583	38.0	35.0	40.0	28.0	41.0
43	35.5129028926259	38.0	35.0	40.0	28.0	41.0
44	35.3721674298881	38.0	34.0	40.0	27.0	41.0
45	35.33432621136117	38.0	34.0	40.0	27.0	41.0
46	35.258899847355714	38.0	34.0	40.0	27.0	41.0
47	35.17586268772939	38.0	34.0	40.0	27.0	41.0
48	35.084769222494636	37.0	34.0	40.0	27.0	41.0
49	34.991486761070995	37.0	34.0	40.0	26.0	41.0
50	34.828308732709715	37.0	34.0	40.0	26.0	41.0
51	34.45861302650261	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	9.0
14	16.0
15	37.0
16	126.0
17	315.0
18	620.0
19	1215.0
20	2340.0
21	3823.0
22	5912.0
23	8772.0
24	13094.0
25	19124.0
26	25962.0
27	30623.0
28	33610.0
29	37797.0
30	45332.0
31	55763.0
32	69226.0
33	91496.0
34	159381.0
35	255459.0
36	139432.0
37	184615.0
38	301918.0
39	614821.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.572028160419766	24.266776711441373	31.21054487870562	13.950650249433236
2	30.12223442297651	25.49659370488676	31.323778667211176	13.057393204925551
3	28.508403051362635	25.757854850370997	32.38448273482208	13.349259363444288
4	25.7148269626833	27.988403413101516	31.85524923570763	14.441520388507556
5	23.02877299600231	32.423702844838296	30.713153920368896	13.834370238790497
6	21.615849493779756	40.60675985250573	27.217756809615402	10.55963384409911
7	89.66583419127325	3.456081806295494	5.596719218758255	1.2813647836730109
8	91.14339471946911	2.3907608082579275	5.185526827459598	1.2803176448133546
9	86.78077624403666	5.0696275945840075	6.332143878783206	1.817452282596124
10	45.6737695999406	35.47059134311266	10.793383795906925	8.06225526103982
11	35.053592090864996	23.21502092135844	26.687142991095037	15.044243996681523
12	33.817016292052735	22.935625234713797	26.912277845921135	16.335080627312333
13	22.975130928055375	32.19523618454966	27.08553172997335	17.74410115742162
14	16.630754563502546	35.02398661946926	29.641026519743562	18.704232297284626
15	15.988001692557175	27.74242097552408	40.13768924060062	16.131888091318125
16	20.31230440517039	24.484724385912553	38.30476786121412	16.898203347702932
17	20.124152590978518	25.07216928617972	28.464470816477967	26.339207306363793
18	21.98639385843539	27.170064394280146	31.89266065132989	18.950881095954568
19	26.997810051866693	27.913580581856984	26.72431642061283	18.36429294566349
20	28.541387925441807	27.45217312251572	27.346364500469544	16.66007445157292
21	24.330271404113258	27.913770970740558	29.312510501136856	18.443447124009325
22	23.48180334446632	24.365969319783357	29.27471830774745	22.877509028002873
23	20.654671215057096	28.565900494201944	29.50551723185987	21.273911058881094
24	20.26937171192448	25.931251526085898	35.5999605895011	18.19941617248852
25	19.759653073376352	28.359614138849658	32.283338640423466	19.597394147350524
26	18.862778640080762	35.23845969681522	26.35953131968531	19.53923034341871
27	18.754304573664555	33.118527075441115	30.58150000737757	17.54566834351676
28	16.783541642570572	30.16845132446407	35.52109199448063	17.526915038484734
29	18.119595633050178	27.556696619597776	34.85658719358694	19.467120553765106
30	19.7061061998712	29.8185165564553	31.85172704136151	18.623650202311985
31	25.998649190871042	28.12058089552267	27.16354357501774	18.717226338588542
32	26.45677244197066	28.188311740854076	27.790684557510055	17.56423125966521
33	24.43251023459242	28.972618746736618	27.044264939458717	19.550606079212248
34	20.15670909006965	29.003033370887543	29.380050957584693	21.46020658145812
35	20.19378732514566	27.721049823342913	30.734334683666493	21.350828167844934
36	24.95588927553698	27.953514650186605	28.482652954859272	18.607943119417143
37	20.517734010546594	32.145116310948836	29.231928406164222	18.105221272340348
38	20.513545455107966	31.295458320779566	26.90609020720498	21.284906016907485
39	20.725638671409254	30.491922037656067	27.855369180704276	20.927070110230403
40	23.107641591022784	27.55883849453798	27.816339459571633	21.517180454867603
41	18.011597538842903	27.231417212011827	29.853024541603062	24.903960707542208
42	21.193614547233818	27.77602461347487	27.12108685398077	23.909273985310545
43	20.966385414688407	27.913961359624135	28.282078266014203	22.837574959673255
44	20.29126643353548	30.01571184261693	28.87870942991381	20.81431229393378
45	19.17972853401036	33.114576506106964	26.162859602953503	21.54283535692918
46	21.331456098941295	31.731496461384612	26.940883775678103	19.996163663995986
47	20.52692027417903	28.429677248004843	28.919928623207547	22.123473854608577
48	21.404184652466512	26.949927247647864	31.023011828385368	20.62287627150026
49	20.52415963536721	27.111567409802078	30.33175738934955	22.032515565481162
50	19.154835187483073	30.84309433340806	29.02564205081194	20.976428428296927
51	18.471767470440938	31.91131876192013	27.501007871152417	22.115905896486517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1320.0
1	2188.5
2	3057.0
3	11748.0
4	20439.0
5	14803.0
6	9167.0
7	8624.5
8	8082.0
9	8339.0
10	8596.0
11	8625.5
12	8655.0
13	8584.0
14	8513.0
15	8037.5
16	7562.0
17	7375.0
18	7188.0
19	7709.5
20	8231.0
21	8739.0
22	9247.0
23	11068.5
24	12890.0
25	14844.5
26	22127.5
27	27456.0
28	32415.5
29	37375.0
30	42413.0
31	47451.0
32	55247.0
33	63043.0
34	71154.5
35	79266.0
36	84028.0
37	88790.0
38	98554.5
39	108319.0
40	130903.5
41	153488.0
42	169954.0
43	186420.0
44	188438.0
45	190456.0
46	184474.5
47	178493.0
48	170531.5
49	162570.0
50	152363.0
51	142156.0
52	127753.0
53	113350.0
54	102085.0
55	90820.0
56	84739.0
57	78658.0
58	74113.5
59	69569.0
60	63859.0
61	58149.0
62	47964.0
63	37779.0
64	31185.0
65	24591.0
66	18914.0
67	13237.0
68	10202.5
69	7168.0
70	5888.0
71	4608.0
72	4169.5
73	3731.0
74	2904.0
75	1633.5
76	1190.0
77	865.5
78	541.0
79	387.5
80	234.0
81	173.5
82	113.0
83	86.5
84	60.0
85	43.5
86	27.0
87	20.0
88	13.0
89	12.0
90	11.0
91	7.5
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2100963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.567394958880733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.36842297258298	15.726006745269144
2	7.017878580225377	2.746432039054782
3	2.2634642423890265	1.328702964183895
4	1.1052922697758811	0.8651076155080968
5	0.7294670174502428	0.7136884619962821
6	0.5102210684747297	0.59902182979123
7	0.3937357598490192	0.5393068185680541
8	0.30767251348106667	0.48162796714204703
9	0.2652282351874283	0.46708430689433855
>10	3.9857294298229524	19.844587524951592
>50	2.0277939963277403	28.667956119823934
>100	1.0219217599888064	26.90048166096276
>500	0.0021961069233247753	0.29412009321141136
>1k	7.320356411082585E-4	0.21080198239097095
>5k	0.0	0.0
>10k+	2.4401188036941946E-4	0.6150738702515262
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12882	0.6131473995496352	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2292	0.10909283028782514	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027320804792849755	0.0
2	0.0	0.0	0.0	0.08362831710982059	0.0
3	0.0	0.0	0.0	0.132748649071878	0.0
4	0.0	0.0	0.0	0.2173289105995679	0.0
5	0.0	0.0	0.0	0.37782673945233686	0.0
6	0.0	0.0	0.0	0.5874449002671632	0.0
7	0.0	0.0	0.0	0.718432452165983	0.0
8	0.0	0.0	0.0	1.0120121106368842	0.0
9	0.0	0.0	0.0	1.1286728990467705	0.0
10	0.0	0.0	0.0	1.3247734491278522	0.0
11	0.0	0.0	0.0	1.5806085114302346	0.0
12	0.0	0.0	0.0	1.7994605330983935	0.0
13	0.0	0.0	0.0	1.889609669470619	0.0
14	0.0	0.0	0.0	1.92930575169577	0.0
15	0.0	0.0	0.0	1.9799491947264183	0.0
16	0.0	0.0	0.0	2.0936589554409095	0.0
17	0.0	0.0	0.0	2.236641007004883	0.0
18	0.0	0.0	0.0	2.4117035854510527	0.0
19	0.0	0.0	0.0	2.519368499112074	0.0
20	0.0	0.0	0.0	2.641502967924709	0.0
21	0.0	0.0	0.0	2.795908352503114	0.0
22	0.0	0.0	0.0	2.965164070000281	0.0
23	0.0	0.0	0.0	3.1415593706314677	0.0
24	0.0	0.0	0.0	3.271356992007951	0.0
25	0.0	0.0	0.0	3.380878197283817	0.0
26	0.0	0.0	0.0	3.491684527523807	0.0
27	0.0	0.0	0.0	3.6017768994503947	0.0
28	0.0	0.0	0.0	3.72391136826303	0.0
29	0.0	0.0	0.0	3.8634188227017803	0.0
30	0.0	0.0	0.0	4.032246165210906	0.0
31	4.7597220893466474E-5	0.0	0.0	4.195457035654602	0.0
32	4.7597220893466474E-5	0.0	0.0	4.3335365734665485	0.0
33	4.7597220893466474E-5	0.0	0.0	4.477375375006604	0.0
34	4.7597220893466474E-5	0.0	0.0	4.62725902360013	0.0
35	4.7597220893466474E-5	0.0	0.0	4.803178352022382	0.0
36	4.7597220893466474E-5	0.0	0.0	4.969435444603261	0.0
37	4.7597220893466474E-5	0.0	0.0	5.13983349540187	0.0
38	4.7597220893466474E-5	0.0	0.0	5.326462198525152	0.0
39	4.7597220893466474E-5	0.0	0.0	5.579774608120181	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGT	20	7.0346217E-4	45.0	38
TACGCGG	125	0.0	43.2	2
TTACGAG	95	0.0	42.63158	1
CGTTTTT	6085	0.0	42.226788	1
CTACGCG	70	0.0	41.785713	1
CGGTCTA	320	0.0	41.484375	31
TATCGCG	60	3.6379788E-12	41.250004	1
CGGTAGT	185	0.0	40.135136	12
TCTCACG	350	0.0	39.857143	23
TAATACG	40	3.4600453E-7	39.375	1
CGCGTGG	40	3.4600453E-7	39.375	2
AGTACCG	40	3.4600453E-7	39.375	1
TAGGGAC	2455	0.0	38.85947	5
AGGGCGA	1230	0.0	38.78049	6
CACGACG	350	0.0	38.571426	26
GGGCGAT	2590	0.0	38.310814	7
CTCACGA	365	0.0	38.219177	24
ATCCGTT	65	9.094947E-12	38.076927	11
TCGCGTA	65	9.094947E-12	38.076927	30
CGTAAGG	290	0.0	38.017242	2
>>END_MODULE