FastQCFastQC Report
Thu 26 May 2016
SRR1548079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548079_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2058722
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504402.4500636802832045No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43690.2122190368587891No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT38620.1875921081136744No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT32470.1577192063814347No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26120.12687482817009776No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC22790.11069974479312894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAC207.034588E-445.00000434
TCCAACG207.034588E-445.00000441
CCCGATA351.2125565E-745.00000436
CCTAGCG253.8918857E-545.01
CGTTTTT190500.044.350391
CGACGGT5750.043.04347628
CGGTCTA5750.043.04347631
TCACGAC6000.042.00000425
TATAGCG1150.041.086961
GCGTAAG1150.041.086961
TAAGTCG556.184564E-1140.9090921
GACGGTC6150.040.60975629
CGGTTTT15300.040.5882341
CTCACGA6250.040.3224
ACGGTCT6200.040.28225730
GCGATAT1850.040.1351369
CGTAAGG3200.040.078132
CCGCTCG1350.040.00000419
TAGGGTA11200.039.977685
ACGGGTA1250.039.65