##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548079_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2058722
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38832100691594	31.0	31.0	33.0	30.0	34.0
2	31.73733316105817	31.0	31.0	34.0	30.0	34.0
3	31.477459316993748	31.0	31.0	34.0	30.0	34.0
4	35.380958186680864	37.0	35.0	37.0	33.0	37.0
5	35.38943140453155	37.0	35.0	37.0	33.0	37.0
6	35.513846939994814	37.0	35.0	37.0	33.0	37.0
7	35.9954384321924	37.0	35.0	37.0	35.0	37.0
8	36.046805736762906	37.0	35.0	37.0	35.0	37.0
9	37.8560825599571	39.0	38.0	39.0	35.0	39.0
10	37.24108888912636	39.0	37.0	39.0	34.0	39.0
11	36.87661957272522	39.0	37.0	39.0	32.0	39.0
12	36.36099920241781	38.0	35.0	39.0	32.0	39.0
13	36.14959814875442	38.0	35.0	39.0	32.0	39.0
14	37.196704071749366	39.0	35.0	41.0	32.0	41.0
15	37.40386074467558	39.0	35.0	41.0	32.0	41.0
16	37.5232216880181	39.0	35.0	41.0	32.0	41.0
17	37.46002131419395	39.0	35.0	41.0	32.0	41.0
18	37.28663170646644	39.0	35.0	41.0	32.0	41.0
19	37.10558734982188	38.0	35.0	41.0	32.0	41.0
20	36.84952946536735	38.0	35.0	41.0	32.0	41.0
21	36.7125323380233	38.0	35.0	41.0	32.0	41.0
22	36.64131582603188	38.0	35.0	40.0	31.0	41.0
23	36.563787631355765	38.0	35.0	40.0	31.0	41.0
24	36.512991554954965	38.0	35.0	40.0	31.0	41.0
25	36.46451633586273	38.0	35.0	40.0	31.0	41.0
26	36.32396651903463	38.0	35.0	40.0	31.0	41.0
27	36.263189979025825	38.0	35.0	40.0	31.0	41.0
28	36.184608218108124	38.0	35.0	40.0	31.0	41.0
29	36.08054657209667	37.0	35.0	40.0	30.0	41.0
30	35.948050781018516	37.0	35.0	40.0	30.0	41.0
31	35.72493906413785	37.0	35.0	40.0	30.0	41.0
32	35.451511665975296	37.0	34.0	40.0	29.0	41.0
33	35.1727348325806	37.0	34.0	40.0	26.0	41.0
34	34.858887212552254	37.0	34.0	40.0	24.0	41.0
35	34.57680735912862	37.0	34.0	40.0	23.0	41.0
36	34.333940668045514	37.0	33.0	40.0	22.0	41.0
37	34.207412171240215	37.0	33.0	40.0	21.0	41.0
38	34.06661948529233	37.0	33.0	40.0	21.0	41.0
39	34.02343638431998	37.0	33.0	40.0	21.0	41.0
40	33.87639079001439	37.0	33.0	40.0	20.0	41.0
41	33.80573433421317	36.0	33.0	40.0	18.0	41.0
42	33.73407337173256	36.0	33.0	40.0	18.0	41.0
43	33.6055654916011	36.0	33.0	40.0	18.0	41.0
44	33.40172398215981	36.0	33.0	40.0	18.0	41.0
45	33.38795864618924	36.0	33.0	40.0	18.0	41.0
46	33.287380714831826	35.0	33.0	40.0	18.0	41.0
47	33.19859213628649	35.0	33.0	40.0	18.0	41.0
48	33.04091421765542	35.0	32.0	40.0	18.0	41.0
49	32.92914099135289	35.0	32.0	40.0	17.0	41.0
50	32.734152547065605	35.0	32.0	40.0	15.0	41.0
51	32.41327338028155	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	6.0
14	23.0
15	74.0
16	170.0
17	470.0
18	1109.0
19	2244.0
20	4067.0
21	6528.0
22	10099.0
23	15630.0
24	23920.0
25	36638.0
26	48198.0
27	51869.0
28	50251.0
29	50808.0
30	55841.0
31	65210.0
32	77693.0
33	97962.0
34	156190.0
35	248279.0
36	156194.0
37	179558.0
38	256964.0
39	462637.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.630480463122264	24.370798971400703	29.000418706362492	17.998301859114537
2	35.95337301490925	24.052737572144274	27.850044833639508	12.143844579306968
3	26.51557616812761	24.15221676360383	36.70957030623853	12.622636762030037
4	23.582542956261214	25.870078621591453	36.92630670872512	13.62107171342221
5	21.193002260625768	30.086577983817143	35.64818367900086	13.072236076556232
6	18.85140392923377	39.497804948895485	32.10414033560626	9.546650786264488
7	82.8174469403834	2.7413609025405083	12.80454573274099	1.636646424335097
8	83.14410590647985	2.213169140855346	12.603790118335551	2.038934834329259
9	78.89826795458542	4.698011679090232	14.081065826274747	2.3226545400496037
10	40.48730231667996	30.658680482357504	19.301488981999512	9.552528218963026
11	30.221856083531435	22.634041895894637	32.52051515454733	14.623586866026594
12	30.69005917263234	21.174009895459417	32.165100484669615	15.970830447238626
13	21.31812843113349	30.66067200913965	32.51492916479253	15.506270394934333
14	15.466925597530896	34.0479676226319	33.55324322565164	16.931863554185558
15	13.720065166642218	28.031468066110914	43.32513083359482	14.923335933652043
16	17.36922226507513	25.337758084870128	41.69358466077499	15.599434989279755
17	17.19736807592283	25.806592633682452	32.5006484605498	24.495390829844922
18	18.893517434602632	27.531011957903985	36.28867812166966	17.286792485823728
19	21.283058130238082	29.645527662307003	30.572801961605307	18.49861224584961
20	23.26967895616795	27.932960351130458	31.2137335686897	17.583627124011887
21	19.54343519911868	29.583790332060378	33.61357191500358	17.259202553817367
22	19.900064214595268	24.940278483447496	33.002707504947246	22.15694979700999
23	16.4236842079698	30.59728316887856	32.159805937858536	20.81922668529311
24	17.28713250259141	28.126041301351034	37.92381875746215	16.6630074385954
25	17.075884942211722	29.596128083344908	34.62376173179283	18.704225242650537
26	16.375838991374263	33.51001252233182	31.220922494635023	18.893225991658902
27	16.557310797669622	33.13303107461814	32.714810450366784	17.59484767734546
28	15.131620490770487	29.447055017627438	37.910218086754796	17.51110640484728
29	16.11951492236446	26.945308788656263	37.09636366639109	19.83881262258819
30	17.80439515388673	30.262512374181654	34.268832800154655	17.664259671776957
31	21.95959435028139	29.32134596123226	30.303751550719333	18.415308137767024
32	22.375920595398505	28.971031542869802	30.89387493794694	17.759172923784757
33	20.8955361627262	30.01779744909706	30.453893240563808	18.632773147612937
34	16.537881268087677	30.925545071165512	32.22610920755692	20.31046445318989
35	16.983206086105845	30.39006723588712	32.670996861159495	19.95572981684754
36	22.034689482115606	29.82593084447536	30.09750709420699	18.04187257920205
37	17.33225758504548	33.43486881667365	31.584740436056936	17.648133162223942
38	16.622788312360775	33.44759515854982	29.526327498321773	20.40328903076763
39	16.99282370324891	33.251891221835685	30.40376505424239	19.351520020673018
40	20.248047089407894	31.009529212783466	28.876166864685953	19.866256833122684
41	16.57868328020976	29.023491272741047	30.82363718850821	23.57418825854098
42	19.272538982922416	29.86469275599134	28.63922375143414	22.223544509652104
43	19.436815655537757	30.51951647672682	29.198211317506683	20.845456550228732
44	18.631753097309883	32.456640576046695	29.10213229372397	19.809474032919454
45	17.166960862127087	35.16220257033247	27.000440078845028	20.670396488695413
46	20.264707911024413	33.22318409187836	27.78145859421525	18.730649402881983
47	18.862964499335025	31.17234867068016	29.039617782294062	20.92506904769075
48	19.05978563400012	29.54386264876948	30.997240035322886	20.399111681907513
49	19.068188905544314	28.278903125336978	31.09754498178967	21.555362987329033
50	17.37330246628734	31.131352363262256	31.261433063813378	20.23391210663703
51	16.559885210339228	33.07032226789241	28.42088441275704	21.94890810901132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1725.0
1	2231.0
2	2737.0
3	33971.5
4	65206.0
5	44284.0
6	23362.0
7	21532.0
8	19702.0
9	21705.0
10	23708.0
11	25622.0
12	27536.0
13	28375.5
14	29215.0
15	28072.5
16	26930.0
17	24914.0
18	22898.0
19	21483.5
20	20069.0
21	18867.5
22	17666.0
23	17748.5
24	17831.0
25	18420.0
26	20994.0
27	22979.0
28	26553.0
29	30127.0
30	35235.5
31	40344.0
32	46220.0
33	52096.0
34	59646.5
35	67197.0
36	69852.0
37	72507.0
38	79588.5
39	86670.0
40	105662.0
41	124654.0
42	148606.5
43	172559.0
44	184580.0
45	196601.0
46	196192.0
47	195783.0
48	181648.5
49	167514.0
50	149755.0
51	131996.0
52	116525.5
53	101055.0
54	90857.0
55	80659.0
56	71565.0
57	62471.0
58	54623.5
59	46776.0
60	39551.5
61	32327.0
62	27027.0
63	21727.0
64	18401.0
65	15075.0
66	11618.0
67	8161.0
68	6313.5
69	4466.0
70	3784.5
71	3103.0
72	2426.5
73	1750.0
74	1410.5
75	781.0
76	491.0
77	420.5
78	350.0
79	317.5
80	285.0
81	202.5
82	120.0
83	94.5
84	69.0
85	66.0
86	63.0
87	51.5
88	40.0
89	29.5
90	19.0
91	16.0
92	13.0
93	8.5
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2058722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.615161369724124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8487163785719	23.05502297977292
2	8.122033873595218	4.810706876337758
3	3.4402556559442568	3.0565117921168588
4	1.9996058010311295	2.368745938934935
5	1.2645042883787734	1.872424927652277
6	0.8999572442858079	1.5991427409225865
7	0.6135525918708872	1.2719321311948169
8	0.5127413408988482	1.214793404131849
9	0.40942382479533196	1.0912637375931071
>10	3.6175373170554503	23.50919660978051
>50	0.9705923625853698	20.454708327265266
>100	0.2966029690129384	11.54426083107042
>500	0.0024868622078710097	0.4912376666877571
>1k	0.0018236989524387406	1.1834875280201596
>5k	0.0	0.0
>10k+	1.657908138580673E-4	2.4765645085187873
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50440	2.4500636802832045	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4369	0.2122190368587891	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	3862	0.1875921081136744	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	3247	0.1577192063814347	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2612	0.12687482817009776	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	2279	0.11069974479312894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8573823954861314E-5	0.0	0.0	0.07310360505206628	0.0
2	4.8573823954861314E-5	0.0	0.0	0.24592927068346285	0.0
3	4.8573823954861314E-5	0.0	0.0	0.44751064009613734	0.0
4	4.8573823954861314E-5	0.0	0.0	0.6410287547323048	0.0
5	4.8573823954861314E-5	0.0	0.0	1.185881338033984	0.0
6	4.8573823954861314E-5	0.0	0.0	2.0586072330309775	0.0
7	4.8573823954861314E-5	0.0	0.0	2.6923984879940077	0.0
8	4.8573823954861314E-5	0.0	0.0	3.7477619610612796	0.0
9	4.8573823954861314E-5	0.0	0.0	4.2722135383019175	0.0
10	4.8573823954861314E-5	0.0	0.0	4.918439692197392	0.0
11	4.8573823954861314E-5	0.0	0.0	5.5165291865535995	0.0
12	4.8573823954861314E-5	0.0	0.0	5.954859373922268	0.0
13	4.8573823954861314E-5	0.0	0.0	6.173490155543099	0.0
14	4.8573823954861314E-5	0.0	0.0	6.276029497911812	0.0
15	4.8573823954861314E-5	0.0	0.0	6.395035366601221	0.0
16	4.8573823954861314E-5	0.0	0.0	6.627315392753369	0.0
17	4.8573823954861314E-5	0.0	0.0	6.9124923131923595	0.0
18	4.8573823954861314E-5	0.0	0.0	7.2867050529406105	0.0
19	4.8573823954861314E-5	0.0	0.0	7.481388939351695	0.0
20	4.8573823954861314E-5	0.0	0.0	7.676947154593966	0.0
21	4.8573823954861314E-5	0.0	0.0	7.931959730356988	0.0
22	4.8573823954861314E-5	0.0	0.0	8.204118865976076	0.0
23	4.8573823954861314E-5	0.0	0.0	8.514117010456001	0.0
24	4.8573823954861314E-5	0.0	0.0	8.737556600648363	0.0
25	4.8573823954861314E-5	0.0	0.0	8.933551980306229	0.0
26	4.8573823954861314E-5	0.0	0.0	9.127992997597538	0.0
27	4.8573823954861314E-5	0.0	0.0	9.324862706086591	0.0
28	4.8573823954861314E-5	0.0	0.0	9.534750199395548	0.0
29	4.8573823954861314E-5	0.0	0.0	9.769993228808941	0.0
30	4.8573823954861314E-5	0.0	0.0	10.059298924284095	0.0
31	4.8573823954861314E-5	0.0	0.0	10.303673832601001	0.0
32	4.8573823954861314E-5	0.0	0.0	10.513172735318319	0.0
33	4.8573823954861314E-5	0.0	0.0	10.742538332033174	0.0
34	4.8573823954861314E-5	0.0	0.0	10.972681109931306	0.0
35	4.8573823954861314E-5	0.0	0.0	11.25227204061549	0.0
36	4.8573823954861314E-5	0.0	0.0	11.499075640130139	0.0
37	4.8573823954861314E-5	0.0	0.0	11.751125212631914	0.0
38	4.8573823954861314E-5	0.0	0.0	12.031687619795193	0.0
39	4.8573823954861314E-5	0.0	0.0	12.375395998099792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAC	20	7.034588E-4	45.000004	34
TCCAACG	20	7.034588E-4	45.000004	41
CCCGATA	35	1.2125565E-7	45.000004	36
CCTAGCG	25	3.8918857E-5	45.0	1
CGTTTTT	19050	0.0	44.35039	1
CGACGGT	575	0.0	43.043476	28
CGGTCTA	575	0.0	43.043476	31
TCACGAC	600	0.0	42.000004	25
TATAGCG	115	0.0	41.08696	1
GCGTAAG	115	0.0	41.08696	1
TAAGTCG	55	6.184564E-11	40.909092	1
GACGGTC	615	0.0	40.609756	29
CGGTTTT	1530	0.0	40.588234	1
CTCACGA	625	0.0	40.32	24
ACGGTCT	620	0.0	40.282257	30
GCGATAT	185	0.0	40.135136	9
CGTAAGG	320	0.0	40.07813	2
CCGCTCG	135	0.0	40.000004	19
TAGGGTA	1120	0.0	39.97768	5
ACGGGTA	125	0.0	39.6	5
>>END_MODULE