FastQCFastQC Report
Thu 26 May 2016
SRR1548078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548078_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1363883
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.28653484206489854No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27950.20492960173269995No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14620.10719394552171997No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA253.8911894E-545.00000416
GTCGATG302.1658016E-645.0000041
GTCGATC207.033749E-445.00000431
GTCGACG302.1658016E-645.0000041
ACGTTAG551.8189894E-1245.0000041
GTTTACG650.045.0000041
TAGGTCG253.8911894E-545.00000428
CACGCAC207.033749E-445.00000445
CATTACG207.033749E-445.00000434
CGTTACA351.2121563E-745.00000438
ACGATTG502.1827873E-1145.0000041
CCGTATG207.033749E-445.00000435
GCGATAT1100.045.0000049
TATAGCG600.045.0000041
ACCGTTG253.8911894E-545.00000428
ACGTAAG650.045.0000041
CACGTAA207.033749E-445.00000419
GTAAGCG502.1827873E-1145.0000041
CGCAACA302.1658016E-645.00000414
TGTCGAT207.033749E-445.00000430