FastQCFastQC Report
Thu 26 May 2016
SRR1548077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548077_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3110137
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337351.0846789064275946No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC62230.20008764887205935No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.0351444E-445.00000427
ATTACGC302.166702E-645.00000434
GCGTAAT302.166702E-645.00000425
CGTTTTT157100.043.281351
CTAGCGG2800.042.5892832
TACGGGA8900.040.7022484
GCGATCG501.0822987E-940.59
TATTGCG3250.040.1538431
CGGTCTA10500.040.07142631
CGAATTA451.9301297E-840.019
CGACGGT10450.039.83253528
CTCACGA10750.039.76744524
CACGACG10550.039.45497526
GGGCGAT38850.039.382247
CGGTCGA403.4607547E-739.37500430
TCACGAC10750.039.3488425
TTACGGG7850.039.2675133
CGGTTTT13100.039.1603051
ATCGGTA2250.039.027
ACGGGAT8950.038.9664845
Produced by FastQC (version 0.11.5)