##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548077_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3110137
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47436720633207	31.0	31.0	33.0	30.0	34.0
2	31.852194613935012	31.0	31.0	34.0	30.0	34.0
3	31.799654484673827	31.0	31.0	34.0	30.0	34.0
4	35.597444099729366	37.0	35.0	37.0	33.0	37.0
5	35.54639586616281	37.0	35.0	37.0	33.0	37.0
6	35.63166027734469	37.0	35.0	37.0	33.0	37.0
7	36.030222141339756	37.0	35.0	37.0	35.0	37.0
8	36.05514290849567	37.0	35.0	37.0	35.0	37.0
9	37.86477187339336	39.0	38.0	39.0	35.0	39.0
10	37.274311067325975	39.0	37.0	39.0	34.0	39.0
11	36.94929612425433	39.0	37.0	39.0	33.0	39.0
12	36.255269140877076	38.0	35.0	39.0	32.0	39.0
13	35.96037441437467	38.0	35.0	39.0	31.0	39.0
14	36.94268837674996	39.0	35.0	41.0	31.0	41.0
15	37.17037577444338	39.0	35.0	41.0	32.0	41.0
16	37.30768451679138	39.0	35.0	41.0	32.0	41.0
17	37.25493539352125	39.0	35.0	41.0	32.0	41.0
18	37.140927875524454	39.0	35.0	41.0	32.0	41.0
19	37.03717971266217	38.0	35.0	41.0	32.0	41.0
20	36.85902003673793	38.0	35.0	41.0	32.0	41.0
21	36.726432308287386	38.0	35.0	41.0	32.0	41.0
22	36.64266815256048	38.0	35.0	41.0	31.0	41.0
23	36.55303640965012	38.0	35.0	40.0	31.0	41.0
24	36.55504435978222	38.0	35.0	40.0	31.0	41.0
25	36.505008300277446	38.0	35.0	40.0	31.0	41.0
26	36.373215070590135	38.0	35.0	40.0	31.0	41.0
27	36.31390450002685	38.0	35.0	40.0	31.0	41.0
28	36.25178601457106	38.0	35.0	40.0	31.0	41.0
29	36.181419982463794	38.0	35.0	40.0	31.0	41.0
30	36.08035047973771	38.0	35.0	40.0	30.0	41.0
31	35.91666540734379	38.0	35.0	40.0	30.0	41.0
32	35.72406295928443	38.0	35.0	40.0	30.0	41.0
33	35.549301204416395	38.0	34.0	40.0	29.0	41.0
34	35.32228194449312	37.0	34.0	40.0	27.0	41.0
35	35.14567268258601	37.0	34.0	40.0	27.0	41.0
36	34.93605137008434	37.0	34.0	40.0	25.0	41.0
37	34.830173397506286	37.0	34.0	40.0	25.0	41.0
38	34.70791736827027	37.0	34.0	40.0	24.0	41.0
39	34.66096702492527	37.0	34.0	40.0	24.0	41.0
40	34.5086419022699	37.0	34.0	40.0	23.0	41.0
41	34.47130464027791	37.0	34.0	40.0	23.0	41.0
42	34.422855327594895	36.0	34.0	40.0	23.0	41.0
43	34.30195550871232	36.0	33.0	40.0	23.0	41.0
44	34.132310248712514	36.0	33.0	40.0	23.0	41.0
45	34.09625460228922	36.0	33.0	40.0	23.0	41.0
46	34.0140058138918	36.0	33.0	40.0	23.0	41.0
47	33.92317701760405	35.0	33.0	40.0	23.0	41.0
48	33.80305176267155	35.0	33.0	40.0	23.0	41.0
49	33.70028426400509	36.0	33.0	40.0	22.0	41.0
50	33.51038491230451	35.0	33.0	40.0	21.0	41.0
51	33.1246118740107	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	3.0
12	11.0
13	21.0
14	40.0
15	114.0
16	263.0
17	638.0
18	1394.0
19	2870.0
20	5130.0
21	8253.0
22	13101.0
23	19056.0
24	28533.0
25	41879.0
26	55022.0
27	61732.0
28	64263.0
29	69714.0
30	80374.0
31	95767.0
32	119314.0
33	154734.0
34	266966.0
35	419499.0
36	222672.0
37	266469.0
38	400891.0
39	711263.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14729769138787	23.999489411559683	28.884676141276096	14.968536755776354
2	32.45689820094742	25.35614990593662	29.34536324284107	12.84158865027489
3	28.456399187559906	24.097973819159733	33.81677398776967	13.628853005510692
4	25.660220112490222	26.721363078218097	33.018577638219796	14.599839171071885
5	22.592445284564636	31.44793943160703	31.64140357804174	14.318211705786593
6	20.94492943558435	39.41813495675592	28.82172071519679	10.815214892462937
7	85.61150200135879	3.6372031199911774	8.831315147853616	1.9199797307964248
8	85.89171473796814	3.048450920329233	8.644860338949698	2.4149740027529334
9	81.62193498228535	4.861039883452079	10.529504005772093	2.9875211284904815
10	45.62824724441399	26.09457396892806	16.594542298297473	11.68263648836048
11	38.18638857387954	21.534839140526607	25.119922369979204	15.15884991561465
12	35.620488743743444	21.039298268854395	27.316803086166303	16.023409901235862
13	22.969084641609037	32.82144805839743	28.175993533403833	16.033473766589704
14	17.072463367369348	35.692414835745176	30.25017868987765	16.984943107007826
15	15.83631203384288	25.16988801457942	43.415064995529136	15.57873495604856
16	18.462530750253123	22.341298791660947	40.93276276897127	18.26340768911466
17	18.99507963797093	23.376912335373007	29.025120115287528	28.602887911368533
18	21.76257830442839	24.918612909977924	33.97284428306534	19.345964502528346
19	25.259658979652666	27.82266504658798	27.30763950269715	19.610036471062205
20	26.781006753078724	26.12341514216255	27.23224089485447	19.86333720990426
21	21.795406440295075	28.821367033027805	29.83762451621906	19.54560201045806
22	22.0906024396996	24.43496862035338	29.288677637030137	24.18575130291688
23	18.787275287230113	29.889840865531003	28.518968778545766	22.803915068693115
24	19.963879404669314	25.734171838732507	35.74868245353822	18.553266303059964
25	19.10854730836616	26.55172424880319	33.477914316957744	20.86181412587291
26	17.903262782314734	31.60548876142755	29.009107958909848	21.482140497347864
27	18.589116813825243	31.280454848130486	30.402937233954646	19.727491104089626
28	16.41139924061223	28.19110540789682	35.98992584571034	19.407569505780614
29	16.967676986576475	26.284887128766353	35.46052794458894	21.286907940068232
30	19.99300995422388	28.829501722914458	32.28079663371742	18.89669168914424
31	25.535981212403186	27.577916985650468	27.368183459442463	19.517918342503883
32	26.087725396019536	27.576759480370157	27.89951053603105	18.436004587579262
33	24.69261000399661	28.060853910937045	27.289344488683298	19.957191596383055
34	18.75087817674913	28.54022186160931	30.807999776215645	21.900900185425915
35	19.44846802568504	27.579267408477502	31.103903139958145	21.86836142587931
36	25.238759578758106	27.012282738670358	28.650313474936954	19.09864420763458
37	20.320616101477203	30.58315437551465	29.359060388658122	19.73716913435003
38	20.299652394733737	30.935871956765894	26.729240544709125	22.035235103791248
39	19.88741975031968	28.877924027140928	29.336135353523012	21.89852086901638
40	22.59852218728628	26.567511334709693	28.04120847409616	22.792758003907867
41	17.629673548142737	25.41444315797021	29.48391019430977	27.471973099577284
42	21.396034965662285	26.663552120051303	27.425158441573473	24.51525447271294
43	20.94525096482888	27.91047468326958	28.13766724745566	23.00660710444588
44	20.47855126639116	29.54699423208688	28.24029938231017	21.73415511921179
45	18.379061758372703	32.979479682084744	25.565851279220176	23.075607280322377
46	21.67505804406687	30.610387902526483	26.920293221809843	20.79426083159681
47	20.72239904544398	27.74366531120655	28.814261236723652	22.719674406625817
48	21.098556108621583	26.554071412288273	30.43891635641774	21.90845612267241
49	20.89335614476147	25.135162856170002	30.56309095065587	23.40839004841266
50	19.53209778218773	29.335556600882857	28.948821225560163	22.183524391369254
51	18.036054360306313	30.844557651318897	27.00733118830457	24.11205680007022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1942.0
1	3179.0
2	4416.0
3	26514.0
4	48612.0
5	34403.5
6	20195.0
7	19366.0
8	18537.0
9	19964.5
10	21392.0
11	22532.0
12	23672.0
13	23073.0
14	22474.0
15	21125.5
16	19777.0
17	17799.0
18	15821.0
19	15442.0
20	15063.0
21	15148.0
22	15233.0
23	15061.5
24	14890.0
25	16061.5
26	22728.0
27	28223.0
28	30145.0
29	32067.0
30	39731.0
31	47395.0
32	53913.0
33	60431.0
34	74021.0
35	87611.0
36	90378.5
37	93146.0
38	108866.5
39	124587.0
40	158942.5
41	193298.0
42	229387.5
43	265477.0
44	285526.5
45	305576.0
46	304152.5
47	302729.0
48	281084.0
49	259439.0
50	242409.5
51	225380.0
52	202882.0
53	180384.0
54	166318.5
55	152253.0
56	139555.0
57	126857.0
58	112860.5
59	98864.0
60	90350.5
61	81837.0
62	71599.0
63	61361.0
64	54171.0
65	46981.0
66	36862.0
67	26743.0
68	22748.0
69	18753.0
70	15462.0
71	12171.0
72	10047.5
73	7924.0
74	6538.5
75	3880.0
76	2607.0
77	2113.5
78	1620.0
79	1231.5
80	843.0
81	662.0
82	481.0
83	368.0
84	255.0
85	215.0
86	175.0
87	152.0
88	129.0
89	99.0
90	69.0
91	47.5
92	26.0
93	24.0
94	22.0
95	14.0
96	6.0
97	4.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3110137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.52137246771024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15418989071	18.618151827992545
2	8.625940472977625	4.057879174984067
3	3.082677587118443	2.175264231734255
4	1.5838937715383725	1.4902142139856163
5	0.9684554406903653	1.1389700569429273
6	0.6743821873902253	0.951743676911679
7	0.4855454120625653	0.7994486140978012
8	0.38286046416209046	0.7204322864573566
9	0.30192172789332866	0.6391452076086278
>10	2.8004387930299353	14.695074155698107
>50	0.9551318956313807	16.553801107070317
>100	0.9783312488098663	35.511199580327165
>500	0.0044310101232252	0.6765078319371157
>1k	0.0015231597298586623	0.6707429729388551
>5k	1.384690663507875E-4	0.20268202003488858
>10k+	1.384690663507875E-4	1.0987430412786385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33735	1.0846789064275946	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6223	0.20008764887205935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06465953107531919	0.0
2	0.0	0.0	0.0	0.21587473477856442	0.0
3	0.0	0.0	0.0	0.3710769011140024	0.0
4	0.0	0.0	0.0	0.5541878058747894	0.0
5	0.0	0.0	0.0	1.0397934238909734	0.0
6	0.0	0.0	0.0	1.726933572379609	0.0
7	0.0	0.0	0.0	2.212892872564778	0.0
8	0.0	0.0	0.0	3.2015952994996684	0.0
9	0.0	0.0	0.0	3.675465100090446	0.0
10	0.0	0.0	0.0	4.343345646831635	0.0
11	0.0	0.0	0.0	4.929300542066153	0.0
12	0.0	0.0	0.0	5.36722337311829	0.0
13	0.0	0.0	0.0	5.573580842258718	0.0
14	0.0	0.0	0.0	5.666084805910479	0.0
15	0.0	0.0	0.0	5.77878080611883	0.0
16	0.0	0.0	0.0	6.029702228551347	0.0
17	0.0	0.0	0.0	6.315380962317737	0.0
18	0.0	0.0	0.0	6.696296658314409	0.0
19	0.0	0.0	0.0	6.887156417868409	0.0
20	0.0	0.0	0.0	7.0848969032553875	0.0
21	0.0	0.0	0.0	7.360061630725592	0.0
22	0.0	0.0	0.0	7.637927203849863	0.0
23	0.0	0.0	0.0	7.964504457520682	0.0
24	0.0	0.0	0.0	8.204140203470137	0.0
25	0.0	0.0	0.0	8.412941294869004	0.0
26	0.0	0.0	0.0	8.608270310921995	0.0
27	0.0	0.0	0.0	8.820351000615085	0.0
28	0.0	0.0	0.0	9.04140235623061	0.0
29	0.0	0.0	0.0	9.281070255104519	0.0
30	0.0	0.0	0.0	9.570703798578649	0.0
31	0.0	0.0	0.0	9.82898824071094	0.0
32	3.2152924453167174E-5	0.0	0.0	10.063125836578903	0.0
33	3.2152924453167174E-5	0.0	0.0	10.305076593088986	0.0
34	3.2152924453167174E-5	0.0	0.0	10.566029727950891	0.0
35	3.2152924453167174E-5	0.0	0.0	10.85942516358604	0.0
36	3.2152924453167174E-5	0.0	0.0	11.11118256205434	0.0
37	3.2152924453167174E-5	0.0	0.0	11.380045316331724	0.0
38	3.2152924453167174E-5	0.0	0.0	11.666560026133897	0.0
39	3.2152924453167174E-5	0.0	0.0	12.075480919329276	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.0351444E-4	45.000004	27
ATTACGC	30	2.166702E-6	45.000004	34
GCGTAAT	30	2.166702E-6	45.000004	25
CGTTTTT	15710	0.0	43.28135	1
CTAGCGG	280	0.0	42.589283	2
TACGGGA	890	0.0	40.702248	4
GCGATCG	50	1.0822987E-9	40.5	9
TATTGCG	325	0.0	40.153843	1
CGGTCTA	1050	0.0	40.071426	31
CGAATTA	45	1.9301297E-8	40.0	19
CGACGGT	1045	0.0	39.832535	28
CTCACGA	1075	0.0	39.767445	24
CACGACG	1055	0.0	39.454975	26
GGGCGAT	3885	0.0	39.38224	7
CGGTCGA	40	3.4607547E-7	39.375004	30
TCACGAC	1075	0.0	39.34884	25
TTACGGG	785	0.0	39.267513	3
CGGTTTT	1310	0.0	39.160305	1
ATCGGTA	225	0.0	39.0	27
ACGGGAT	895	0.0	38.966484	5
>>END_MODULE