FastQCFastQC Report
Thu 26 May 2016
SRR1548076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548076_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2378805
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80580.3387415109687427No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76480.3215059662309437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA453.8562575E-1045.00000424
ATCGGAT351.2126657E-745.00000434
CTTAACG406.8212103E-945.01
CGAACTA207.0348097E-445.023
CGAAACG207.0348097E-445.045
CGGCATA207.0348097E-445.027
CCAATCG551.8189894E-1245.024
ATTCGCA207.0348097E-445.043
CGCGACT253.892069E-545.022
CCGTACC253.892069E-545.018
AACGCGT253.892069E-545.022
TATTACG406.8212103E-945.01
GAACGTA502.1827873E-1145.036
ACCGTAC253.892069E-545.019
GCTAACG750.045.01
TGTCGAC253.892069E-545.043
CCCGTAG302.1664855E-644.99999618
GCGATAA2450.041.326539
CGCGATT603.6379788E-1241.24999643
GTGTACG603.6379788E-1241.2499961