FastQCFastQC Report
Thu 26 May 2016
SRR1548075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548075_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1415364
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.3142654469097702No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20980.14823041987785474No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16260.11488210806548703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG406.8175723E-945.01
TACGGAT207.033841E-445.016
TCGCGCG207.033841E-445.01
CGTTTTT27550.040.9981881
CGACGGT2150.040.81395328
ATAGGGC4450.040.4494364
ACTAACG451.9288564E-840.01
ACGGGCC800.039.3755
TATCGCG403.458972E-739.3751
GGCGATA3150.039.2857138
TCACGAC2250.039.00000425
CACGACG2250.039.00000426
TCGATAC356.2493145E-638.5714325
GCTACGA1950.038.07692310
AGTACGG1300.038.076922
CGAGGGT1600.037.968754
GGGCGAT13100.037.9580157
AGGGCGA7550.037.8476836
TAAGGGA11600.037.8232774
CGCATCG601.5643309E-1037.49999621
Produced by FastQC (version 0.11.5)