##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548075_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1415364
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.485831913203953	31.0	31.0	33.0	30.0	34.0
2	31.888707781178553	31.0	31.0	34.0	30.0	34.0
3	31.98675464403503	33.0	31.0	34.0	30.0	34.0
4	35.71755958184608	37.0	35.0	37.0	35.0	37.0
5	35.6341732586105	37.0	35.0	37.0	33.0	37.0
6	35.68268940004126	37.0	35.0	37.0	33.0	37.0
7	35.99410681633841	37.0	35.0	37.0	35.0	37.0
8	35.985699085182326	37.0	35.0	37.0	35.0	37.0
9	37.73150864371286	39.0	37.0	39.0	35.0	39.0
10	37.25637927769817	39.0	37.0	39.0	34.0	39.0
11	36.939116015385444	39.0	37.0	39.0	33.0	39.0
12	36.26463722406392	38.0	35.0	39.0	32.0	39.0
13	36.015742240158716	38.0	35.0	39.0	32.0	39.0
14	37.01899228749636	39.0	35.0	41.0	32.0	41.0
15	37.19547904284693	39.0	35.0	41.0	32.0	41.0
16	37.30548749297001	39.0	35.0	41.0	32.0	41.0
17	37.22166029374776	39.0	35.0	41.0	32.0	41.0
18	37.125815691228546	39.0	35.0	41.0	32.0	41.0
19	37.10614654604752	39.0	35.0	41.0	32.0	41.0
20	36.9906285591551	39.0	35.0	41.0	32.0	41.0
21	36.859768229232905	38.0	35.0	41.0	31.0	41.0
22	36.7783905765584	38.0	35.0	41.0	31.0	41.0
23	36.703771609282136	38.0	35.0	41.0	31.0	41.0
24	36.719850158687095	38.0	35.0	41.0	31.0	41.0
25	36.66754417944783	38.0	35.0	41.0	31.0	41.0
26	36.54836282398026	38.0	35.0	41.0	31.0	41.0
27	36.49401496717452	38.0	35.0	41.0	31.0	41.0
28	36.40106855904205	38.0	35.0	40.0	31.0	41.0
29	36.326708182488744	38.0	35.0	40.0	30.0	41.0
30	36.24187276205979	38.0	35.0	40.0	30.0	41.0
31	36.15545470988382	38.0	35.0	40.0	30.0	41.0
32	36.03287069616014	38.0	35.0	40.0	30.0	41.0
33	35.92719187431643	38.0	35.0	40.0	30.0	41.0
34	35.774351332943326	38.0	35.0	40.0	29.0	41.0
35	35.656570323959066	38.0	35.0	40.0	29.0	41.0
36	35.51583691545073	38.0	34.0	40.0	27.0	41.0
37	35.45989370932142	38.0	34.0	40.0	27.0	41.0
38	35.36995783416845	38.0	34.0	40.0	27.0	41.0
39	35.35530294680379	38.0	34.0	40.0	27.0	41.0
40	35.21625956291103	38.0	34.0	40.0	27.0	41.0
41	35.16084837540025	38.0	34.0	40.0	26.0	41.0
42	35.09878589535978	37.0	34.0	40.0	26.0	41.0
43	34.99612043262369	37.0	34.0	40.0	26.0	41.0
44	34.82272051571186	37.0	34.0	40.0	25.0	41.0
45	34.78679901424651	37.0	34.0	40.0	25.0	41.0
46	34.69392537891313	37.0	34.0	40.0	25.0	41.0
47	34.61952261043802	37.0	34.0	40.0	24.0	41.0
48	34.52057774537151	36.0	34.0	40.0	24.0	41.0
49	34.415665510780265	36.0	33.0	40.0	24.0	41.0
50	34.257309073849555	36.0	33.0	40.0	24.0	41.0
51	33.89731122170692	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	7.0
12	17.0
13	25.0
14	53.0
15	82.0
16	185.0
17	410.0
18	761.0
19	1578.0
20	2513.0
21	3803.0
22	5671.0
23	7885.0
24	11292.0
25	15339.0
26	19486.0
27	22824.0
28	25495.0
29	28981.0
30	34209.0
31	41580.0
32	51857.0
33	68637.0
34	120597.0
35	173589.0
36	95344.0
37	125205.0
38	196074.0
39	361795.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.690561579918665	23.49763029157164	27.299549797790533	12.512258330719165
2	32.548517554494815	24.79680138819413	28.55505721496378	14.09962384234727
3	30.42842689230474	25.605074030426096	29.726557973779183	14.239941103489986
4	27.330213287889194	27.10419369151681	28.899703539160242	16.66588948143375
5	24.062714609104088	31.79754465988961	28.395663589013143	15.744077141993154
6	22.851012177786068	40.24915145503206	26.422460935844068	10.477375431337805
7	85.91549594309308	4.7797598356323885	6.677363561599702	2.627380659674826
8	85.64383437758767	4.433841753782066	6.950932763585904	2.971391105044356
9	80.52091193502167	6.6014113683829745	8.92434737636396	3.9533293202314033
10	46.355990402468905	26.91660943757224	14.865080643565895	11.862319516392956
11	37.87266031918291	24.420007856636172	22.42553858936641	15.281793234814506
12	35.469744885414634	22.70906989297453	25.839501357954564	15.981683863656276
13	24.645744840196585	32.62630673098934	25.283884569623076	17.44406385919099
14	18.480758306697076	34.518823426341214	28.722646612461528	18.277771654500185
15	18.288864207369976	25.219872767712054	39.75542687252184	16.735836152396132
16	22.202839693534667	22.628383935157316	36.821128699048444	18.347647672259573
17	21.87274792915462	23.216147930850298	27.48550902806628	27.425595111928804
18	23.698002775257816	24.65061991120305	32.22224106307635	19.42913625046278
19	27.313185865968048	27.40482307024907	25.86408867259588	19.417902391187003
20	28.94039978408381	25.745179331959832	26.25579002998522	19.058630853971135
21	24.76543136606555	27.5679613159583	28.376021998581287	19.29058531939487
22	23.743432784781866	24.860671883699176	27.944330928298307	23.451564403220658
23	21.89260147919546	27.135351754036417	27.565064534635614	23.406982232132513
24	22.399326251056266	24.70742508640887	33.156841632258555	19.73640703027631
25	20.694605769257944	26.716590219901025	30.445524967428874	22.143279043412154
26	19.48714253011946	30.65204427977538	27.690544623149947	22.170268566955215
27	19.365407061363722	30.18170590745561	29.003210481543974	21.449676549636703
28	17.988446788246698	28.482496375490683	32.67922598003058	20.84983085623204
29	19.49201760112593	26.259040077322865	32.21800187089682	22.03094045065439
30	21.104394346613308	28.29102619538154	30.808470471200337	19.796108986804807
31	26.488733640250846	26.49014670431069	26.661198108755062	20.359921546683395
32	27.379317263968844	26.345166331770486	26.39045503488855	19.88506136937212
33	26.42133048459619	27.73759965634282	25.535198012666704	20.305871846394286
34	20.66938257578969	27.94263525142649	29.015362832458646	22.372619340325176
35	20.534646917683368	28.264743203868402	29.963387510209387	21.237222368238843
36	25.520007574023364	28.78729429319949	26.242931147040622	19.44976698573653
37	22.05114726671019	30.443264064933118	27.206075610231718	20.299513058124976
38	20.77387866301531	30.343148476293024	26.820097162284757	22.062875698406913
39	21.164308262750783	29.67886706175938	27.717746106301984	21.439078569187856
40	23.65370321698164	26.46767898575914	27.102709974253976	22.775907823005248
41	19.035527256592648	26.549000822403286	28.380826416384757	26.03464550461931
42	21.810997029739347	26.722313129343405	26.323475798451845	25.143214042465402
43	21.755251652578416	26.70012802360382	26.967903662944654	24.576716660873103
44	20.41213426369471	28.532377536803256	27.797584225683288	23.25790397381875
45	19.5849972162638	31.948742514293144	25.529475103224332	22.936785166218726
46	21.778849822377847	30.36116504305606	26.738563366031638	21.121421768534454
47	20.84015136742209	28.2417809128959	28.165687413273194	22.752380306408813
48	21.88165023273165	25.640541938328234	30.41019836593272	22.067609463007397
49	21.52506351722949	25.240150236970845	30.092329605670344	23.142456640129325
50	19.73301567653268	29.61951837124584	28.57307378172682	22.074392170494658
51	19.436342877167995	29.522016951116463	27.320109879861292	23.72153029185425
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	679.0
1	1543.5
2	2408.0
3	5623.0
4	8838.0
5	6698.0
6	4558.0
7	4455.0
8	4352.0
9	4386.5
10	4421.0
11	4368.5
12	4316.0
13	4238.5
14	4161.0
15	4287.5
16	4414.0
17	4357.5
18	4301.0
19	4533.0
20	4765.0
21	5509.5
22	6254.0
23	6775.5
24	7297.0
25	8135.0
26	10811.0
27	12649.0
28	15446.5
29	18244.0
30	21754.5
31	25265.0
32	28610.5
33	31956.0
34	38695.0
35	45434.0
36	50049.0
37	54664.0
38	60551.5
39	66439.0
40	78472.0
41	90505.0
42	102851.5
43	115198.0
44	114366.5
45	113535.0
46	113902.5
47	114270.0
48	112045.0
49	109820.0
50	105315.5
51	100811.0
52	95329.0
53	89847.0
54	81740.0
55	73633.0
56	69473.5
57	65314.0
58	60907.0
59	56500.0
60	52577.5
61	48655.0
62	43221.0
63	37787.0
64	32032.0
65	26277.0
66	21934.0
67	17591.0
68	14599.0
69	11607.0
70	9708.0
71	7809.0
72	6139.5
73	4470.0
74	3645.5
75	2422.0
76	2023.0
77	1610.5
78	1198.0
79	883.0
80	568.0
81	437.0
82	306.0
83	254.0
84	202.0
85	145.5
86	89.0
87	73.5
88	58.0
89	43.5
90	29.0
91	24.5
92	20.0
93	16.0
94	12.0
95	11.5
96	11.0
97	10.5
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1415364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.54938210194745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0521797706926	19.087287516157755
2	5.636149337805248	2.6545566867922776
3	1.8182664909167372	1.2845715707329624
4	1.0356507637661816	0.975557422404141
5	0.6879557963811602	0.8100466959114748
6	0.4939205346402882	0.6978914038945391
7	0.4138912131683822	0.682281762927857
8	0.3532768653160733	0.665556151128515
9	0.31013659306586433	0.6573172620513802
>10	6.174059840625073	37.65566493541374
>50	1.8879613822454615	30.06875562758516
>100	0.13534831524139507	4.143438289348444
>500	3.007740338642739E-4	0.03824843030899489
>1k	9.023221015928217E-4	0.5788262453427893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4448	0.3142654469097702	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2098	0.14823041987785474	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1626	0.11488210806548703	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4130640598460892E-4	0.0	0.0	0.02444600823533734	0.0
2	1.4130640598460892E-4	0.0	0.0	0.0825229410950116	0.0
3	1.4130640598460892E-4	0.0	0.0	0.12427898406346354	0.0
4	1.4130640598460892E-4	0.0	0.0	0.19952464525026778	0.0
5	1.4130640598460892E-4	0.0	0.0	0.36739665555998313	0.0
6	1.4130640598460892E-4	0.0	0.0	0.5805573689877657	0.0
7	1.4130640598460892E-4	0.0	0.0	0.7131734310043212	0.0
8	1.4130640598460892E-4	0.0	0.0	1.0894723901413346	0.0
9	1.4130640598460892E-4	0.0	0.0	1.2442029046944814	0.0
10	1.4130640598460892E-4	0.0	0.0	1.5100002543515307	0.0
11	1.4130640598460892E-4	0.0	0.0	1.7661887684016266	0.0
12	1.4130640598460892E-4	0.0	0.0	1.9772298857396402	0.0
13	1.4130640598460892E-4	0.0	0.0	2.0736008546211435	0.0
14	1.4130640598460892E-4	0.0	0.0	2.115286244386603	0.0
15	1.4130640598460892E-4	0.0	0.0	2.1795806591096	0.0
16	1.4130640598460892E-4	0.0	0.0	2.3158000344787633	0.0
17	1.4130640598460892E-4	0.0	0.0	2.4624054306877947	0.0
18	1.4130640598460892E-4	0.0	0.0	2.6600224394572702	0.0
19	1.4130640598460892E-4	0.0	0.0	2.7643065670739118	0.0
20	1.4130640598460892E-4	0.0	0.0	2.878270183500499	0.0
21	1.4130640598460892E-4	0.0	0.0	3.0288321590770995	0.0
22	1.4130640598460892E-4	0.0	0.0	3.1852583505020617	0.0
23	1.4130640598460892E-4	0.0	0.0	3.3539781992476847	0.0
24	1.4130640598460892E-4	0.0	0.0	3.4880779785270786	0.0
25	1.4130640598460892E-4	0.0	0.0	3.603313352607527	0.0
26	1.4130640598460892E-4	0.0	0.0	3.718407420281991	0.0
27	1.4130640598460892E-4	0.0	0.0	3.8291916425739245	0.0
28	1.4130640598460892E-4	0.0	0.0	3.9480303300069806	0.0
29	1.4130640598460892E-4	0.0	0.0	4.082554028504328	0.0
30	1.4130640598460892E-4	0.0	0.0	4.26229577691675	0.0
31	1.4130640598460892E-4	0.0	0.0	4.410172930779644	0.0
32	1.4130640598460892E-4	0.0	0.0	4.552963054027091	0.0
33	1.4130640598460892E-4	0.0	0.0	4.698720611800216	0.0
34	1.4130640598460892E-4	0.0	0.0	4.855570722443131	0.0
35	1.4130640598460892E-4	0.0	0.0	5.033334181171769	0.0
36	1.4130640598460892E-4	0.0	0.0	5.192586500716423	0.0
37	1.4130640598460892E-4	0.0	0.0	5.357844342515423	0.0
38	1.4130640598460892E-4	0.0	0.0	5.55857009221656	0.0
39	1.4130640598460892E-4	0.0	0.0	5.821823926565887	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	40	6.8175723E-9	45.0	1
TACGGAT	20	7.033841E-4	45.0	16
TCGCGCG	20	7.033841E-4	45.0	1
CGTTTTT	2755	0.0	40.998188	1
CGACGGT	215	0.0	40.813953	28
ATAGGGC	445	0.0	40.449436	4
ACTAACG	45	1.9288564E-8	40.0	1
ACGGGCC	80	0.0	39.375	5
TATCGCG	40	3.458972E-7	39.375	1
GGCGATA	315	0.0	39.285713	8
TCACGAC	225	0.0	39.000004	25
CACGACG	225	0.0	39.000004	26
TCGATAC	35	6.2493145E-6	38.57143	25
GCTACGA	195	0.0	38.076923	10
AGTACGG	130	0.0	38.07692	2
CGAGGGT	160	0.0	37.96875	4
GGGCGAT	1310	0.0	37.958015	7
AGGGCGA	755	0.0	37.847683	6
TAAGGGA	1160	0.0	37.823277	4
CGCATCG	60	1.5643309E-10	37.499996	21
>>END_MODULE