FastQCFastQC Report
Thu 26 May 2016
SRR1548074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548074_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2590480
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102930.39733948920663353No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42870.1654905654550508No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA253.8921644E-545.00000416
CGCAATA253.8921644E-545.00000430
TAGACGT207.034927E-445.028
CGCCGAT351.2127202E-745.029
GCGATAT900.042.5000049
CGTTTTT71450.040.906231
TTGTACG1050.040.7142871
TCACGAC4400.040.39772825
CGACGGT4350.040.3448328
TATAGCG900.040.0000041
TAACGGG6100.039.8360673
CGTAAGG5000.039.62
CGGTCTA4450.039.438231
CGAGGGA13100.039.332064
TACGGGA8200.038.6890264
GAGTACG1000.038.2500041
CACGACG4600.038.15217626
CGGGATT6200.038.104846
CGTTAGG3350.037.6119422
AAGGGAC21350.037.5175675
Produced by FastQC (version 0.11.5)