##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548074_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2590480
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51348977795621	31.0	31.0	33.0	30.0	34.0
2	31.91452433525833	31.0	31.0	34.0	30.0	34.0
3	31.97107756091535	33.0	31.0	34.0	30.0	34.0
4	35.69832000247059	37.0	35.0	37.0	33.0	37.0
5	35.62182221055557	37.0	35.0	37.0	33.0	37.0
6	35.69016823137025	37.0	35.0	37.0	33.0	37.0
7	36.035582208702635	37.0	35.0	37.0	35.0	37.0
8	36.04769733794509	37.0	35.0	37.0	35.0	37.0
9	37.8325839226707	39.0	38.0	39.0	35.0	39.0
10	37.31923967758871	39.0	37.0	39.0	34.0	39.0
11	36.98841990673543	39.0	37.0	39.0	33.0	39.0
12	36.2610207374695	38.0	35.0	39.0	32.0	39.0
13	35.96470731293042	38.0	35.0	39.0	32.0	39.0
14	36.969177912973656	39.0	35.0	41.0	31.0	41.0
15	37.172268845928166	39.0	35.0	41.0	32.0	41.0
16	37.31337744356258	39.0	35.0	41.0	32.0	41.0
17	37.237102776319446	39.0	35.0	41.0	32.0	41.0
18	37.15640885086934	39.0	35.0	41.0	32.0	41.0
19	37.094126571137394	38.0	35.0	41.0	32.0	41.0
20	36.97737832370835	38.0	35.0	41.0	32.0	41.0
21	36.82267340415676	38.0	35.0	41.0	32.0	41.0
22	36.75359199839412	38.0	35.0	41.0	31.0	41.0
23	36.679783283406934	38.0	35.0	41.0	31.0	41.0
24	36.685288440752295	38.0	35.0	41.0	31.0	41.0
25	36.655266205490875	38.0	35.0	41.0	31.0	41.0
26	36.50999930514808	38.0	35.0	40.0	31.0	41.0
27	36.432168555634476	38.0	35.0	40.0	31.0	41.0
28	36.37673249745221	38.0	35.0	40.0	31.0	41.0
29	36.326788857663445	38.0	35.0	40.0	31.0	41.0
30	36.22075599888824	38.0	35.0	40.0	30.0	41.0
31	36.106623868935486	38.0	35.0	40.0	30.0	41.0
32	35.96231818041444	38.0	35.0	40.0	30.0	41.0
33	35.82626270034897	38.0	35.0	40.0	30.0	41.0
34	35.658483369877395	38.0	35.0	40.0	29.0	41.0
35	35.535570241808465	38.0	34.0	40.0	28.0	41.0
36	35.37196967357401	38.0	34.0	40.0	27.0	41.0
37	35.27387163768877	38.0	34.0	40.0	27.0	41.0
38	35.17398281399586	38.0	34.0	40.0	27.0	41.0
39	35.148724174670335	38.0	34.0	40.0	26.0	41.0
40	35.0091770637102	37.0	34.0	40.0	25.0	41.0
41	34.965528010252925	37.0	34.0	40.0	25.0	41.0
42	34.90928592384423	37.0	34.0	40.0	25.0	41.0
43	34.78296184490905	37.0	34.0	40.0	25.0	41.0
44	34.62542926407461	37.0	34.0	40.0	24.0	41.0
45	34.575899447206695	37.0	34.0	40.0	24.0	41.0
46	34.51080147308607	36.0	34.0	40.0	24.0	41.0
47	34.43872718569531	36.0	33.0	40.0	24.0	41.0
48	34.34026203637936	36.0	33.0	40.0	23.0	41.0
49	34.24755875359007	36.0	33.0	40.0	24.0	41.0
50	34.07862403878818	36.0	33.0	40.0	24.0	41.0
51	33.70486627960841	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	12.0
12	26.0
13	38.0
14	61.0
15	128.0
16	225.0
17	624.0
18	1304.0
19	2512.0
20	4414.0
21	7049.0
22	10330.0
23	15032.0
24	21798.0
25	30388.0
26	38578.0
27	44764.0
28	48245.0
29	54405.0
30	63377.0
31	76392.0
32	94830.0
33	124246.0
34	216941.0
35	337750.0
36	169963.0
37	222377.0
38	352679.0
39	651887.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.25905623668201	23.770806954695654	28.7079228559958	13.262213952626539
2	32.186274358420064	24.963327259812853	29.245236404064112	13.605161977702974
3	30.874200920292765	24.587450974336804	30.7112195423242	13.827128563046232
4	26.27543930082456	27.43217473209598	30.3976869151663	15.894699051913157
5	23.351425218492324	32.380601278527536	29.057433371421514	15.21054013155863
6	22.36288255458448	40.28407862635496	26.882469658132855	10.470569160927704
7	86.95002470584602	4.2509882338408325	6.699105957197121	2.099881103116025
8	87.2603532935981	3.573855038448473	6.713968067694018	2.4518236002594116
9	82.39361045057287	6.084625243198172	8.236581637379944	3.285182668849016
10	45.83664031376424	29.817408356752416	14.083258701090145	10.262692628393195
11	39.60482227232019	22.396235446712577	23.665305271609895	14.33363700935734
12	36.16600012352923	21.814567184460024	26.532959142707142	15.486473549303604
13	23.933711126895403	33.10587227077607	26.63757295945153	16.322843642876997
14	18.311664247552578	36.23471325777462	29.430375837682593	16.02324665699021
15	16.947592724128345	25.82756863592848	42.309764985639724	14.915073654303448
16	20.57020320558352	22.854953522127172	39.95529785985609	16.61954541243322
17	20.441037954355952	21.873629597603532	29.118464531669808	28.566867916370715
18	23.734520243352584	24.240025014669094	32.74161545350668	19.283839288471636
19	27.183456347858314	26.114465272845187	27.158634693184275	19.543443686112226
20	29.432807819400264	25.61309101016028	26.08902597202063	18.865075198418825
21	24.040255087860167	27.418740928322165	29.492565084463113	19.04843889935456
22	23.599410147926253	23.60446712578364	29.24685772520923	23.54926500108088
23	20.79552824187023	28.442180599734414	28.148875884006053	22.613415274389304
24	21.421821438497883	25.10144838022297	34.46608350575955	19.010646675519595
25	19.84118773354745	26.036487446341987	32.48413421450851	21.638190605602052
26	19.336763842994348	32.02977826503196	28.00318859825206	20.630269293721625
27	19.370811587041782	31.559710941601555	29.59710169543868	19.47237577591798
28	17.35616565269757	28.84110280720175	35.10940057441092	18.693330965689757
29	19.957189401192057	25.56715357771533	33.94811000277941	20.527547018313207
30	20.26261542262438	28.14003582347673	31.772451437571412	19.824897316327476
31	26.679843117877766	26.07462709613662	26.57553812420864	20.66999166177697
32	27.541382292084865	26.93010561749174	27.565856520799237	17.96265556962416
33	25.759627559371236	27.682591643247584	26.971873938420675	19.585906858960502
34	20.712570643278465	27.913629906426607	29.773015039683763	21.60078441061116
35	20.398922207467344	27.94771625335845	29.865546153608598	21.787815385565608
36	26.749096692504864	26.527554738890093	27.544470522837468	19.17887804576758
37	21.979208486458106	29.982898922207468	28.13208362928878	19.905808962045644
38	21.24270405484698	30.826950989777956	25.159198295296626	22.771146660078443
39	21.1817501003675	28.96984342670084	28.02241283468701	21.82599363824465
40	22.730536425681727	26.28103671906365	27.31393409715574	23.674492758098886
41	18.604235508477192	26.16190821778203	28.748417281739293	26.48543899200148
42	23.214462184614433	26.479339736265096	26.28879589882956	24.01740218029091
43	22.45510484543405	27.103123745406254	26.684359655353447	23.757411753806245
44	21.253976097093975	29.40092183687965	27.923357833297302	21.421744232729072
45	19.380462308143663	32.27753157715944	25.733918038355824	22.608088076341065
46	22.040509866897253	30.120711219542322	27.3845387727371	20.45424014082332
47	21.61313733362157	28.371846144343905	27.4471526512461	22.567863870788425
48	22.50914888360458	26.09446897872209	29.362936598622646	22.03344553905068
49	21.825453197862945	25.03505141904203	29.78420987616195	23.355285506933075
50	19.869676662240202	30.193593465303724	27.804576757975358	22.13215311448071
51	18.911823291436335	31.659615206448226	26.645139124795403	22.783422377320033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1301.0
1	2779.0
2	4257.0
3	12141.5
4	20026.0
5	15243.0
6	10460.0
7	10071.0
8	9682.0
9	9926.5
10	10171.0
11	10118.5
12	10066.0
13	9846.0
14	9626.0
15	9171.0
16	8716.0
17	8744.5
18	8773.0
19	9308.0
20	9843.0
21	10534.5
22	11226.0
23	13409.0
24	15592.0
25	16339.0
26	22571.5
27	28057.0
28	32219.5
29	36382.0
30	39189.0
31	41996.0
32	50043.0
33	58090.0
34	67071.0
35	76052.0
36	84347.0
37	92642.0
38	104495.5
39	116349.0
40	145147.0
41	173945.0
42	192890.5
43	211836.0
44	220375.0
45	228914.0
46	219229.5
47	209545.0
48	211594.0
49	213643.0
50	204442.5
51	195242.0
52	182612.5
53	169983.0
54	153814.5
55	137646.0
56	125168.0
57	112690.0
58	102435.0
59	92180.0
60	85398.0
61	78616.0
62	68985.5
63	59355.0
64	50185.5
65	41016.0
66	33719.5
67	26423.0
68	21117.5
69	15812.0
70	13047.5
71	10283.0
72	8724.5
73	7166.0
74	5871.0
75	3611.5
76	2647.0
77	1956.5
78	1266.0
79	876.0
80	486.0
81	426.5
82	367.0
83	267.5
84	168.0
85	138.0
86	108.0
87	85.5
88	63.0
89	53.0
90	43.0
91	40.0
92	37.0
93	31.0
94	25.0
95	15.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2590480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.089529002888614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0567337687936	16.48481132930489
2	6.988456416439597	2.8078959572697264
3	2.127347581218144	1.2821223279632052
4	1.069592898847731	0.859504702507408
5	0.6268047269735528	0.6296105870841434
6	0.46051462265651216	0.5550913120867386
7	0.3120944637569904	0.438888154690092
8	0.2613233438953017	0.41998903170532
9	0.2170907485274076	0.3925125799920138
>10	2.9169154180827674	14.935447422335232
>50	1.753237370201263	26.03773475709305
>100	1.205829910132376	34.113521027877766
>500	0.0036721847152767135	0.476764238322354
>1k	1.9327287975140595E-4	0.1664539693531991
>5k	0.0	0.0
>10k+	1.9327287975140595E-4	0.3996526024148538
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10293	0.39733948920663353	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4287	0.1654905654550508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0284503258083444	0.0
2	0.0	0.0	0.0	0.08797597356474476	0.0
3	0.0	0.0	0.0	0.13375899447206696	0.0
4	0.0	0.0	0.0	0.22748679781353262	0.0
5	0.0	0.0	0.0	0.40641116704240143	0.0
6	0.0	0.0	0.0	0.6438189061486674	0.0
7	0.0	0.0	0.0	0.7868425928785399	0.0
8	0.0	0.0	0.0	1.200974336802446	0.0
9	0.0	0.0	0.0	1.3775825329668634	0.0
10	0.0	0.0	0.0	1.6625876285476051	0.0
11	0.0	0.0	0.0	1.9272104011611748	0.0
12	0.0	0.0	0.0	2.1508755134183626	0.0
13	0.0	0.0	0.0	2.246186035020537	0.0
14	0.0	0.0	0.0	2.2858311973070626	0.0
15	0.0	0.0	0.0	2.3439285383403847	0.0
16	0.0	0.0	0.0	2.4705846020814675	0.0
17	0.0	0.0	0.0	2.6162332849510515	0.0
18	0.0	0.0	0.0	2.8230289367221517	0.0
19	0.0	0.0	0.0	2.9296887063401376	0.0
20	0.0	0.0	0.0	3.050284117229239	0.0
21	0.0	0.0	0.0	3.191339056854328	0.0
22	0.0	0.0	0.0	3.352853525215404	0.0
23	0.0	0.0	0.0	3.5144838022297025	0.0
24	0.0	0.0	0.0	3.64059942558908	0.0
25	0.0	0.0	0.0	3.740773910626602	0.0
26	0.0	0.0	0.0	3.8530310984836786	0.0
27	0.0	0.0	0.0	3.9630879219295267	0.0
28	0.0	0.0	0.0	4.086539946264785	0.0
29	0.0	0.0	0.0	4.2197971032395545	0.0
30	0.0	0.0	0.0	4.395517433062598	0.0
31	0.0	0.0	0.0	4.550816837034064	0.0
32	0.0	0.0	0.0	4.694535375683271	0.0
33	0.0	0.0	0.0	4.838755751829777	0.0
34	0.0	0.0	0.0	4.991854791390013	0.0
35	0.0	0.0	0.0	5.169466662549025	0.0
36	0.0	0.0	0.0	5.324573052098453	0.0
37	0.0	0.0	0.0	5.490411043513172	0.0
38	0.0	0.0	0.0	5.684120317470121	0.0
39	0.0	0.0	0.0	5.9473533862450205	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	25	3.8921644E-5	45.000004	16
CGCAATA	25	3.8921644E-5	45.000004	30
TAGACGT	20	7.034927E-4	45.0	28
CGCCGAT	35	1.2127202E-7	45.0	29
GCGATAT	90	0.0	42.500004	9
CGTTTTT	7145	0.0	40.90623	1
TTGTACG	105	0.0	40.714287	1
TCACGAC	440	0.0	40.397728	25
CGACGGT	435	0.0	40.34483	28
TATAGCG	90	0.0	40.000004	1
TAACGGG	610	0.0	39.836067	3
CGTAAGG	500	0.0	39.6	2
CGGTCTA	445	0.0	39.4382	31
CGAGGGA	1310	0.0	39.33206	4
TACGGGA	820	0.0	38.689026	4
GAGTACG	100	0.0	38.250004	1
CACGACG	460	0.0	38.152176	26
CGGGATT	620	0.0	38.10484	6
CGTTAGG	335	0.0	37.611942	2
AAGGGAC	2135	0.0	37.517567	5
>>END_MODULE