FastQCFastQC Report
Thu 26 May 2016
SRR1548073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548073_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3917355
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170260.4346299990682489No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54060.13800127892417205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA5700.042.23684331
TACGTAG1300.041.538461
GGGCGAT52650.040.641027
GCGATAC2050.040.6097569
CGACGGT7000.040.50000428
GGCGATA9950.040.251268
CGTTTTT98800.040.194841
AGGGCGA28200.040.0531926
TAAGGGA44000.039.2727284
TACGGGA14500.039.258624
GTATGCG2300.039.1304321
TAACGCA356.252145E-638.57142631
TCACGAC6550.037.7862625
TTAGGGA41800.037.62564
CGATTCG1200.037.49999610
GGCGATT17600.037.0738648
TAGGGAG39950.036.9461825
TAGGGAC33900.036.9026575
ACGGGAC8050.036.8944135
CACGACG6650.036.87969626
Produced by FastQC (version 0.11.5)