##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548073_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3917355
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.505202107033956	31.0	31.0	33.0	30.0	34.0
2	31.916082407644954	31.0	31.0	34.0	30.0	34.0
3	31.98035511205903	33.0	31.0	34.0	30.0	34.0
4	35.71578909749053	37.0	35.0	37.0	35.0	37.0
5	35.6348850180798	37.0	35.0	37.0	33.0	37.0
6	35.69185917538748	37.0	35.0	37.0	33.0	37.0
7	36.033074612844636	37.0	35.0	37.0	35.0	37.0
8	36.03318540188469	37.0	35.0	37.0	35.0	37.0
9	37.79548419788352	39.0	38.0	39.0	35.0	39.0
10	37.29906582375097	39.0	37.0	39.0	34.0	39.0
11	36.99705515583857	39.0	37.0	39.0	33.0	39.0
12	36.43826918928716	38.0	35.0	39.0	32.0	39.0
13	36.222993576022596	39.0	35.0	39.0	32.0	39.0
14	37.28414427592087	39.0	35.0	41.0	32.0	41.0
15	37.441208162140015	39.0	35.0	41.0	32.0	41.0
16	37.54534143573917	39.0	35.0	41.0	33.0	41.0
17	37.45945057315459	39.0	35.0	41.0	32.0	41.0
18	37.36792274379013	39.0	35.0	41.0	32.0	41.0
19	37.321379349076096	39.0	35.0	41.0	32.0	41.0
20	37.19573411140936	39.0	35.0	41.0	32.0	41.0
21	37.073043163052624	39.0	35.0	41.0	32.0	41.0
22	36.98286675575739	39.0	35.0	41.0	32.0	41.0
23	36.86908947491356	39.0	35.0	41.0	31.0	41.0
24	36.8936379266112	39.0	35.0	41.0	31.0	41.0
25	36.85591502429573	39.0	35.0	41.0	31.0	41.0
26	36.72006928144118	38.0	35.0	41.0	31.0	41.0
27	36.64272576776932	38.0	35.0	41.0	31.0	41.0
28	36.561512806472734	38.0	35.0	41.0	31.0	41.0
29	36.47877534714112	38.0	35.0	41.0	31.0	41.0
30	36.39346140444254	38.0	35.0	40.0	30.0	41.0
31	36.324281307157506	38.0	35.0	41.0	30.0	41.0
32	36.203883487710456	38.0	35.0	41.0	30.0	41.0
33	36.10680216625759	38.0	35.0	41.0	30.0	41.0
34	35.97356762407288	38.0	35.0	41.0	30.0	41.0
35	35.84319368553527	38.0	35.0	41.0	29.0	41.0
36	35.68575939632737	38.0	35.0	40.0	28.0	41.0
37	35.600790073914666	38.0	35.0	40.0	28.0	41.0
38	35.48877597256312	38.0	34.0	40.0	27.0	41.0
39	35.43351981119914	38.0	34.0	40.0	27.0	41.0
40	35.26899936309066	38.0	34.0	40.0	26.0	41.0
41	35.2026750192413	38.0	34.0	40.0	26.0	41.0
42	35.16073013551235	38.0	34.0	40.0	26.0	41.0
43	35.064845539911495	38.0	34.0	40.0	26.0	41.0
44	34.89452117564019	37.0	34.0	40.0	25.0	41.0
45	34.83897195939607	37.0	34.0	40.0	25.0	41.0
46	34.745924737482305	37.0	34.0	40.0	24.0	41.0
47	34.6633800102365	37.0	34.0	40.0	24.0	41.0
48	34.54112762310283	37.0	34.0	40.0	24.0	41.0
49	34.445250175181975	36.0	34.0	40.0	24.0	41.0
50	34.27406426019597	36.0	33.0	40.0	24.0	41.0
51	33.89903212754525	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	21.0
12	24.0
13	48.0
14	82.0
15	184.0
16	341.0
17	798.0
18	1804.0
19	3526.0
20	6165.0
21	9603.0
22	14332.0
23	20679.0
24	29280.0
25	40744.0
26	53157.0
27	62956.0
28	70023.0
29	78900.0
30	93547.0
31	113508.0
32	140980.0
33	181691.0
34	303512.0
35	451525.0
36	277490.0
37	365664.0
38	565812.0
39	1030739.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.97412284564457	24.182873392888823	28.796190286558147	13.046813474908452
2	31.938846492084583	25.59438702900299	28.88773164545976	13.579034833452674
3	29.823311903057036	25.212011676245833	30.669571688039504	14.295104732657624
4	26.870018162765437	27.770830062631546	29.789028566468957	15.57012320813406
5	24.396384805563958	31.976218647531308	27.768277319773166	15.859119227131572
6	22.753388447051645	40.98076380619066	25.53924778326192	10.726599963495778
7	87.02354011826857	4.326720452958693	6.589854634057929	2.0598847947148013
8	86.98144538853384	3.797153946987189	6.585336279198592	2.636064385280374
9	81.29855476463072	6.471101036285963	8.500097642414334	3.7302465566689773
10	42.80967642707898	32.32469357512914	13.580847280882125	11.284782716909751
11	35.62995950073456	22.709225995601624	24.76053357431226	16.900280929351567
12	33.01503182632159	21.79243392544204	27.642707898569313	17.549826349667057
13	24.120203555715527	29.910590181385142	27.9140644644154	18.055141798483927
14	18.757171611967767	33.00334026403019	29.283968391937925	18.95551973206411
15	18.129605307662953	25.642735978740756	38.89708744803573	17.33057126556056
16	21.91402106778681	23.208568026129875	36.386745648530706	18.490665257552607
17	21.46583600414055	23.280504319879103	28.392601640647836	26.861058035332512
18	22.981731295733983	24.99704520014142	31.66343106509367	20.357792439030927
19	26.34134511679437	26.24620439046244	26.697886711824687	20.714563780918503
20	28.005069747316746	25.245758936833656	26.856922591901935	19.892248723947663
21	24.88112514694226	27.014809737692907	28.516715998422406	19.587349116942427
22	24.26867108035907	23.396628592506936	28.435742994954506	23.898957332179492
23	21.666022099094924	27.80225432721824	27.79482584550034	22.73689772818649
24	21.748501220849274	25.161186565935434	33.02572781889821	20.064584394317084
25	20.91589861015915	26.3818571459569	30.65399995660337	22.048244287280575
26	20.2478202766918	30.180210882087533	27.467385519055586	22.104583322165084
27	20.613755965440966	29.314626833667106	29.024073641525977	21.04754355936595
28	18.851597570299347	27.567325401961273	33.07017107206266	20.510905955676726
29	20.095957604046607	25.37625515175418	32.96068393086662	21.56710331333259
30	20.9709102187573	27.266101744672106	30.85959786641752	20.903390170153074
31	25.665736191894784	26.02794487607072	27.044472609707316	21.261846322327184
32	25.68424357761806	26.95160382451935	27.628923087134048	19.73522951072854
33	24.426124259864118	26.916095171359245	27.52594544022689	21.131835128549746
34	20.36713547789261	26.949204246232473	29.544526855493057	23.139133420381867
35	21.166909815423928	25.95460457374938	30.718686460634792	22.1597991501919
36	25.373600299181465	26.212355020160288	27.69963406431125	20.714410616347
37	21.69594024539517	28.564733091588586	28.786796192839304	20.952530470176946
38	22.26663143881522	28.99987363922851	25.96718959604121	22.766305325915063
39	21.418967645260643	27.857725429530895	27.618022875128755	23.10528405007971
40	23.63035772862046	25.940692125171193	27.507080670503438	22.92186947570491
41	19.706740900428986	25.346464642596857	28.74209766538902	26.204696791585135
42	21.966224659240737	26.47528753457371	26.98841437653723	24.57007342964832
43	22.342294737137685	26.128369780119492	27.53975577909074	23.98957970365208
44	21.129027111405527	28.001521434743598	28.301162391460565	22.568289062390313
45	20.2831757652804	29.908777733955695	26.71802785297733	23.090018647786582
46	22.150558221044555	28.892760548890767	27.54113426023427	21.41554696983041
47	21.76902527343067	27.027190540556063	28.505381820131188	22.69840236588208
48	22.099248089591065	25.307356622006434	29.684825602989772	22.908569685412733
49	21.779414936864285	24.632641157107283	30.207499703243645	23.380444202784787
50	20.12383355606015	28.20729803655783	28.999286508371082	22.66958189901094
51	19.962857591410533	28.914714137472863	27.262144993241616	23.860283277874995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2325.0
1	4570.0
2	6815.0
3	19341.0
4	31867.0
5	23748.0
6	15629.0
7	14552.0
8	13475.0
9	13747.0
10	14019.0
11	14170.5
12	14322.0
13	14115.0
14	13908.0
15	13287.0
16	12666.0
17	12267.0
18	11868.0
19	12032.0
20	12196.0
21	13168.5
22	14141.0
23	15072.0
24	16003.0
25	19812.0
26	27896.0
27	32171.0
28	40788.0
29	49405.0
30	58701.5
31	67998.0
32	79502.5
33	91007.0
34	101423.0
35	111839.0
36	122251.0
37	132663.0
38	148256.5
39	163850.0
40	195057.5
41	226265.0
42	264112.0
43	301959.0
44	306767.0
45	311575.0
46	325246.0
47	338917.0
48	335598.5
49	332280.0
50	318092.5
51	303905.0
52	279207.5
53	254510.0
54	232107.0
55	209704.0
56	198343.0
57	186982.0
58	171354.0
59	155726.0
60	142883.0
61	130040.0
62	115280.5
63	100521.0
64	87918.0
65	75315.0
66	62031.0
67	48747.0
68	40899.0
69	33051.0
70	27038.5
71	21026.0
72	17960.0
73	14894.0
74	12122.0
75	7019.0
76	4688.0
77	3680.0
78	2672.0
79	2158.5
80	1645.0
81	1280.5
82	916.0
83	659.5
84	403.0
85	320.0
86	237.0
87	168.5
88	100.0
89	95.5
90	91.0
91	55.0
92	19.0
93	17.0
94	15.0
95	11.5
96	8.0
97	7.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3917355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.169468735952456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82747499921315	16.302472300026082
2	7.940569274785303	3.2031412746689365
3	2.6191364529836183	1.584797724109395
4	1.2054126161789167	0.9725012830377325
5	0.7258629286778964	0.7320134823277858
6	0.4796591538474071	0.5804682184463212
7	0.33951677513948136	0.4793511087055019
8	0.27183936598354513	0.4386284474725001
9	0.19714674688196768	0.35787106328675217
>10	2.615246427271363	13.16132180970441
>50	1.4669211421341624	21.81297556112523
>100	1.3043176247024435	38.5583454021889
>500	0.004086810193061159	0.5368690155959183
>1k	0.0025542563706632245	0.7014174884692858
>5k	1.277128185331612E-4	0.13925313781362317
>10k+	1.277128185331612E-4	0.43857268302159597
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17026	0.4346299990682489	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5406	0.13800127892417205	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.552742858382761E-5	0.0	0.0	0.04684283145132366	0.0
2	2.552742858382761E-5	0.0	0.0	0.16728123950982232	0.0
3	2.552742858382761E-5	0.0	0.0	0.26893146013062386	0.0
4	2.552742858382761E-5	0.0	0.0	0.39319898247669666	0.0
5	2.552742858382761E-5	0.0	0.0	0.6992728511967897	0.0
6	2.552742858382761E-5	0.0	0.0	1.0519087496537842	0.0
7	2.552742858382761E-5	0.0	0.0	1.265037250900161	0.0
8	2.552742858382761E-5	0.0	0.0	1.820769371170088	0.0
9	2.552742858382761E-5	0.0	0.0	2.052022346710982	0.0
10	2.552742858382761E-5	0.0	0.0	2.4274797663219188	0.0
11	2.552742858382761E-5	0.0	0.0	2.8024011099325947	0.0
12	2.552742858382761E-5	0.0	0.0	3.110364008367891	0.0
13	2.552742858382761E-5	0.0	0.0	3.254160013580592	0.0
14	2.552742858382761E-5	0.0	0.0	3.3139197238953324	0.0
15	2.552742858382761E-5	0.0	0.0	3.401810660509451	0.0
16	2.552742858382761E-5	0.0	0.0	3.5966104680326394	0.0
17	5.105485716765522E-5	0.0	0.0	3.8117301087085544	0.0
18	5.105485716765522E-5	0.0	0.0	4.089621696272102	0.0
19	5.105485716765522E-5	0.0	0.0	4.233341119199052	0.0
20	5.105485716765522E-5	0.0	0.0	4.395389235849189	0.0
21	5.105485716765522E-5	0.0	0.0	4.590010351372291	0.0
22	5.105485716765522E-5	0.0	0.0	4.8053597389054605	0.0
23	5.105485716765522E-5	0.0	0.0	5.039190984733321	0.0
24	5.105485716765522E-5	0.0	0.0	5.212471169960343	0.0
25	5.105485716765522E-5	0.0	0.0	5.367269496892674	0.0
26	5.105485716765522E-5	0.0	0.0	5.518111072394511	0.0
27	5.105485716765522E-5	0.0	0.0	5.672373323326581	0.0
28	5.105485716765522E-5	0.0	0.0	5.838505828550131	0.0
29	5.105485716765522E-5	0.0	0.0	6.014874832635797	0.0
30	5.105485716765522E-5	0.0	0.0	6.228079916167925	0.0
31	7.658228575148283E-5	0.0	0.0	6.420684364832904	0.0
32	7.658228575148283E-5	0.0	0.0	6.600167715205796	0.0
33	7.658228575148283E-5	0.0	0.0	6.781820897008313	0.0
34	7.658228575148283E-5	0.0	0.0	6.972127877100748	0.0
35	7.658228575148283E-5	0.0	0.0	7.181401736630966	0.0
36	7.658228575148283E-5	0.0	0.0	7.3819452155855165	0.0
37	7.658228575148283E-5	0.0	0.0	7.589330045400532	0.0
38	7.658228575148283E-5	0.0	0.0	7.809887028364802	0.0
39	7.658228575148283E-5	0.0	0.0	8.067075871346866	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	570	0.0	42.236843	31
TACGTAG	130	0.0	41.53846	1
GGGCGAT	5265	0.0	40.64102	7
GCGATAC	205	0.0	40.609756	9
CGACGGT	700	0.0	40.500004	28
GGCGATA	995	0.0	40.25126	8
CGTTTTT	9880	0.0	40.19484	1
AGGGCGA	2820	0.0	40.053192	6
TAAGGGA	4400	0.0	39.272728	4
TACGGGA	1450	0.0	39.25862	4
GTATGCG	230	0.0	39.130432	1
TAACGCA	35	6.252145E-6	38.571426	31
TCACGAC	655	0.0	37.78626	25
TTAGGGA	4180	0.0	37.6256	4
CGATTCG	120	0.0	37.499996	10
GGCGATT	1760	0.0	37.073864	8
TAGGGAG	3995	0.0	36.946182	5
TAGGGAC	3390	0.0	36.902657	5
ACGGGAC	805	0.0	36.894413	5
CACGACG	665	0.0	36.879696	26
>>END_MODULE