FastQCFastQC Report
Thu 26 May 2016
SRR1548072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548072_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2604066
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87900.33754904829601096No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC83810.3218428411568678No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.0349325E-445.00000443
CTACCGT406.8212103E-945.00000416
ACGTATA351.2127202E-745.00000444
ACCGTAC302.1665637E-645.00000416
ATACGTA302.1665637E-645.00000445
TCGGTCG207.0349325E-445.0000041
TACACCG600.045.00000432
ACGCGTC207.0349325E-445.00000414
CGAACTA502.1827873E-1144.99999614
CGATAAT253.892172E-544.99999613
TGCGTAG900.042.51
TAGTCCG750.042.0000041
TTGTGCG2450.041.326531
TACGACG603.6379788E-1241.2500041
ACGAATC603.6379788E-1241.25000418
TCACGAC5100.041.0294125
CACGACG5100.041.0294126
CGACGGT5050.040.990128
AATCGTC556.184564E-1140.9090939
CGGTCTA5150.040.63106531