##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548072_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2604066
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52459576677396	31.0	31.0	33.0	30.0	34.0
2	31.922994655281393	31.0	31.0	34.0	30.0	34.0
3	32.006632320378976	33.0	31.0	34.0	30.0	34.0
4	35.72064648131038	37.0	35.0	37.0	35.0	37.0
5	35.61801313791586	37.0	35.0	37.0	33.0	37.0
6	35.68132681736945	37.0	35.0	37.0	33.0	37.0
7	36.047311012854514	37.0	35.0	37.0	35.0	37.0
8	36.06790112078573	37.0	35.0	37.0	35.0	37.0
9	37.86093132816142	39.0	38.0	39.0	35.0	39.0
10	37.32102450552328	39.0	37.0	39.0	34.0	39.0
11	36.96318987306773	39.0	37.0	39.0	33.0	39.0
12	36.08246334770317	37.0	35.0	39.0	32.0	39.0
13	35.68500337549048	37.0	35.0	39.0	31.0	39.0
14	36.67647594185401	38.0	35.0	41.0	31.0	41.0
15	36.93914785569951	38.0	35.0	41.0	32.0	41.0
16	37.11190192568084	38.0	35.0	41.0	32.0	41.0
17	37.0445445699149	38.0	35.0	41.0	32.0	41.0
18	37.00982809191472	38.0	35.0	41.0	32.0	41.0
19	36.964977078153936	38.0	35.0	41.0	32.0	41.0
20	36.84135809153839	38.0	35.0	41.0	32.0	41.0
21	36.68061677392201	38.0	35.0	41.0	32.0	41.0
22	36.574278839322815	38.0	35.0	41.0	31.0	41.0
23	36.528113726764225	38.0	35.0	40.0	31.0	41.0
24	36.53548565973366	38.0	35.0	40.0	31.0	41.0
25	36.50823135819138	38.0	35.0	40.0	31.0	41.0
26	36.370210662863386	38.0	35.0	40.0	31.0	41.0
27	36.295267477859625	38.0	35.0	40.0	31.0	41.0
28	36.25509722103818	38.0	35.0	40.0	31.0	41.0
29	36.224366049093994	38.0	35.0	40.0	31.0	41.0
30	36.169266831178625	37.0	35.0	40.0	31.0	41.0
31	36.05233815118357	38.0	35.0	40.0	30.0	41.0
32	35.90343601122245	37.0	35.0	40.0	30.0	41.0
33	35.81160615744762	37.0	35.0	40.0	30.0	41.0
34	35.71527257757676	37.0	35.0	40.0	30.0	41.0
35	35.59009141857388	37.0	35.0	40.0	29.0	41.0
36	35.41883270239694	37.0	34.0	40.0	29.0	41.0
37	35.30674875367982	37.0	34.0	40.0	28.0	41.0
38	35.23870746747586	37.0	34.0	40.0	28.0	41.0
39	35.23124490700312	37.0	34.0	40.0	27.0	41.0
40	35.084924498841424	37.0	34.0	40.0	27.0	41.0
41	35.08320219226395	37.0	34.0	40.0	27.0	41.0
42	35.0421694381018	36.0	34.0	40.0	27.0	41.0
43	34.89667888601902	36.0	34.0	40.0	27.0	41.0
44	34.73283012028113	36.0	34.0	40.0	26.0	41.0
45	34.69113263642319	36.0	34.0	40.0	26.0	41.0
46	34.62056414852773	36.0	34.0	40.0	26.0	41.0
47	34.53859080376611	35.0	34.0	40.0	26.0	41.0
48	34.43984100249379	35.0	34.0	40.0	26.0	41.0
49	34.368431906103766	36.0	34.0	40.0	25.0	41.0
50	34.175365754938625	35.0	33.0	40.0	24.0	41.0
51	33.801896342105	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	9.0
13	17.0
14	50.0
15	70.0
16	181.0
17	458.0
18	1031.0
19	2107.0
20	3732.0
21	6037.0
22	9099.0
23	13358.0
24	19267.0
25	26460.0
26	34468.0
27	41329.0
28	46998.0
29	54045.0
30	64497.0
31	79246.0
32	99367.0
33	130339.0
34	242751.0
35	402440.0
36	164693.0
37	211560.0
38	329187.0
39	621124.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.77087984713137	24.278493709452835	29.60232190735565	12.348304536060146
2	29.44748712206219	26.736303918564275	30.30188174954091	13.514327209832624
3	29.685729931576233	25.454462367697282	30.869801303039168	13.99000639768731
4	26.623787569132272	28.075210075320673	29.753623756079918	15.547378599467141
5	23.767561958875085	32.84144871904168	28.736560440480385	14.654428881602847
6	22.37109197693146	41.54445394241159	25.01699265686814	11.067461423788798
7	88.3375843776617	4.352347444342809	5.48158149601431	1.828486681981179
8	89.24489625070947	3.351796767055827	5.20931497127953	2.1939920109551756
9	84.39106382096307	5.850504557104159	6.95447043200902	2.8039611899237578
10	49.97814955534921	28.671316318403605	11.705694095310948	9.644840030936235
11	43.66198859783124	19.893428200360514	22.09483169781411	14.349751503994138
12	39.94987070220186	19.856101957477268	24.81442482640609	15.379602513914778
13	23.111779808960296	36.83839042482026	24.48363443937289	15.566195326846554
14	16.702572054625346	39.891078029512315	27.117822666553	16.288527249309347
15	14.615796988248379	25.80687279047459	44.89026007789357	14.687070143383462
16	17.898586287751538	20.95688050917296	43.376934378775346	17.76759882430015
17	17.67962102343028	20.740680151731947	28.66171594729166	32.917982877546116
18	21.688121576027644	24.555944434588064	33.16736211755001	20.588571871834276
19	26.854119672850075	25.29905156013711	26.944324759817917	20.902504007194903
20	29.976160358454816	23.548097475256004	26.078140876613727	20.397601289675453
21	22.9956537199902	27.627448766659523	28.445745998757328	20.931151514592948
22	22.915049004134303	24.03172577039138	27.106417425672007	25.946807799802308
23	19.558452051522504	29.42947682585618	25.934596127747916	25.0774749948734
24	20.163390636028428	24.772989624686932	35.968443196140186	19.09517654314445
25	17.665719686060186	25.68863461985987	34.09491157290176	22.550734121178188
26	17.289999562223077	34.47397262588582	27.35591187012925	20.880115941761844
27	18.549184237265877	33.63778030203535	28.682567953346805	19.130467507351963
28	16.235648405224754	28.78605995393358	36.996297328869545	17.981994311972123
29	17.653815225881374	24.79242077581751	35.83906859503561	21.71469540326551
30	19.663288104064954	29.40102132588037	30.91572947843872	20.019961091615958
31	28.067760187337804	27.031649735452174	24.08456621299153	20.816023864218494
32	28.509991682238468	28.092490743322173	26.295186066712596	17.102331507726763
33	26.819942351691545	28.476159974439973	25.573622173938755	19.130275499929724
34	20.548787933946375	28.39478722889512	28.41871135370609	22.637713483452416
35	20.86321928860482	26.67497674790117	30.779519413102435	21.682284550391582
36	29.06220502859759	25.22317022686829	27.542735091967714	18.17188965256641
37	21.416546278012923	31.062960769811514	29.475212993833487	18.04527995834207
38	20.1932669909288	32.36676796978264	24.746953418231335	22.693011621057224
39	20.641066701074397	30.192859935193656	28.48007692585365	20.685996437878305
40	22.884174210638285	27.24723566914203	26.766257076433547	23.102333043786142
41	17.493220217920744	25.06468730055229	29.435621063367822	28.00647141815914
42	23.263312066591247	25.369364678161	25.96205318912808	25.405270066119677
43	23.378631724387937	26.09058295757481	26.905270450134523	23.625514867902734
44	20.146647588809195	30.404068099656463	27.852059049194605	21.59722526233974
45	18.612969102933643	34.96163307688822	24.07630989383526	22.349087926342882
46	23.002297176799665	31.924190861521947	25.454193557306155	19.61931840437224
47	21.930396541408705	27.89168938114472	27.259677750103105	22.918236327343468
48	22.904104581066687	25.19767164119496	30.232797479019347	21.665426298719005
49	21.08556388355748	23.908341800860654	31.726192807709175	23.279901507872687
50	19.864281473664647	30.75812978626502	28.38468763848535	20.992901101584984
51	18.96272982328405	31.776690759758008	25.246902344257023	24.013677072700922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1527.0
1	2800.5
2	4074.0
3	9644.0
4	15214.0
5	11531.0
6	7848.0
7	7579.5
8	7311.0
9	7637.0
10	7963.0
11	8008.5
12	8054.0
13	7717.5
14	7381.0
15	7588.0
16	7795.0
17	7343.5
18	6892.0
19	7036.5
20	7181.0
21	7341.5
22	7502.0
23	9413.5
24	11325.0
25	13275.0
26	18792.0
27	22359.0
28	27644.0
29	32929.0
30	38974.5
31	45020.0
32	52606.0
33	60192.0
34	66734.5
35	73277.0
36	75672.0
37	78067.0
38	95008.5
39	111950.0
40	150923.0
41	189896.0
42	225442.0
43	260988.0
44	266952.5
45	272917.0
46	264507.5
47	256098.0
48	239690.5
49	223283.0
50	202438.5
51	181594.0
52	162846.0
53	144098.0
54	131269.5
55	118441.0
56	108105.5
57	97770.0
58	93678.5
59	89587.0
60	80577.0
61	71567.0
62	64173.5
63	56780.0
64	47543.5
65	38307.0
66	30947.0
67	23587.0
68	19635.0
69	15683.0
70	12361.5
71	9040.0
72	7853.0
73	6666.0
74	5477.5
75	3034.5
76	1780.0
77	1539.5
78	1299.0
79	968.5
80	638.0
81	482.0
82	326.0
83	240.0
84	154.0
85	113.5
86	73.0
87	58.0
88	43.0
89	36.5
90	30.0
91	25.5
92	21.0
93	17.5
94	14.0
95	10.0
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2604066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.405165337621433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76326085448036	14.8646116923216
2	8.582652276275123	3.1593026836031317
3	2.714079930249895	1.4985926956730806
4	1.2138007526501111	0.8936081415781851
5	0.665153391902604	0.6121129076423566
6	0.4374454710519908	0.4830753732543348
7	0.3175060875913513	0.4090626426474093
8	0.2302520155758592	0.3390261132795419
9	0.1800211668048548	0.29819874053833917
>10	1.9817467467201608	9.055883610089117
>50	1.2127147043750388	16.667772633807093
>100	1.692773841880057	49.92531842233897
>500	0.007335283304601614	0.9189740567177869
>1k	8.383180919544702E-4	0.2121154440014737
>5k	4.191590459772351E-4	0.6623448425076024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8790	0.33754904829601096	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8381	0.3218428411568678	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.840148444778281E-5	0.0	0.0	0.047041818448533945	0.0
2	3.840148444778281E-5	0.0	0.0	0.18079418878016149	0.0
3	3.840148444778281E-5	0.0	0.0	0.2670823243343295	0.0
4	3.840148444778281E-5	0.0	0.0	0.4135455860181731	0.0
5	3.840148444778281E-5	0.0	0.0	0.7299354163834557	0.0
6	3.840148444778281E-5	0.0	0.0	1.0446739829174836	0.0
7	3.840148444778281E-5	0.0	0.0	1.2321116285071116	0.0
8	3.840148444778281E-5	0.0	0.0	1.7606696604463943	0.0
9	3.840148444778281E-5	0.0	0.0	1.9468016555647976	0.0
10	3.840148444778281E-5	0.0	0.0	2.284734718705286	0.0
11	3.840148444778281E-5	0.0	0.0	2.6526593411994934	0.0
12	3.840148444778281E-5	0.0	0.0	2.9596024063906214	0.0
13	3.840148444778281E-5	0.0	0.0	3.0928555574244276	0.0
14	3.840148444778281E-5	0.0	0.0	3.1422398664242763	0.0
15	3.840148444778281E-5	0.0	0.0	3.2355554736323886	0.0
16	3.840148444778281E-5	0.0	0.0	3.4402353857390713	0.0
17	3.840148444778281E-5	0.0	0.0	3.677863771501951	0.0
18	3.840148444778281E-5	0.0	0.0	4.011342262446497	0.0
19	3.840148444778281E-5	0.0	0.0	4.160340022103894	0.0
20	3.840148444778281E-5	0.0	0.0	4.321357446393447	0.0
21	3.840148444778281E-5	0.0	0.0	4.525192525842279	0.0
22	3.840148444778281E-5	0.0	0.0	4.7353254487405465	0.0
23	3.840148444778281E-5	0.0	0.0	4.970419336529873	0.0
24	3.840148444778281E-5	0.0	0.0	5.1491782466343015	0.0
25	3.840148444778281E-5	0.0	0.0	5.2938366385491	0.0
26	3.840148444778281E-5	0.0	0.0	5.449746665407098	0.0
27	3.840148444778281E-5	0.0	0.0	5.586916767854579	0.0
28	3.840148444778281E-5	0.0	0.0	5.739101850721141	0.0
29	3.840148444778281E-5	0.0	0.0	5.897200762192663	0.0
30	3.840148444778281E-5	0.0	0.0	6.094469187800923	0.0
31	3.840148444778281E-5	0.0	0.0	6.282367651203925	0.0
32	7.680296889556563E-5	0.0	0.0	6.4487996848006155	0.0
33	7.680296889556563E-5	0.0	0.0	6.619532684655458	0.0
34	1.5360593779113125E-4	0.0	0.0	6.795104271550721	0.0
35	1.5360593779113125E-4	0.0	0.0	7.02029057635252	0.0
36	1.9200742223891406E-4	0.0	0.0	7.219402273214273	0.0
37	1.9200742223891406E-4	0.0	0.0	7.415403449835757	0.0
38	2.304089066866969E-4	0.0	0.0	7.608178901763627	0.0
39	2.304089066866969E-4	0.0	0.0	7.796614985948897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	20	7.0349325E-4	45.000004	43
CTACCGT	40	6.8212103E-9	45.000004	16
ACGTATA	35	1.2127202E-7	45.000004	44
ACCGTAC	30	2.1665637E-6	45.000004	16
ATACGTA	30	2.1665637E-6	45.000004	45
TCGGTCG	20	7.0349325E-4	45.000004	1
TACACCG	60	0.0	45.000004	32
ACGCGTC	20	7.0349325E-4	45.000004	14
CGAACTA	50	2.1827873E-11	44.999996	14
CGATAAT	25	3.892172E-5	44.999996	13
TGCGTAG	90	0.0	42.5	1
TAGTCCG	75	0.0	42.000004	1
TTGTGCG	245	0.0	41.32653	1
TACGACG	60	3.6379788E-12	41.250004	1
ACGAATC	60	3.6379788E-12	41.250004	18
TCACGAC	510	0.0	41.02941	25
CACGACG	510	0.0	41.02941	26
CGACGGT	505	0.0	40.9901	28
AATCGTC	55	6.184564E-11	40.90909	39
CGGTCTA	515	0.0	40.631065	31
>>END_MODULE