FastQCFastQC Report
Thu 26 May 2016
SRR1548071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548071_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences773852
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.3623431870693621No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13700.17703643590764123No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG253.8896193E-545.0000041
TAATCGG302.1645792E-645.0000042
TCGTTAG207.031857E-445.01
CATACGA207.031857E-445.018
TCACGAC2150.041.86046625
CGACGGT2200.040.90909228
CGGTCTA2200.040.90909231
TAGGGCG2750.040.0909045
GGGCGAT9350.039.946527
CGTTTTT17300.039.7976881
GCGTAGG2100.039.6428572
GGCGATA2000.039.3750048
TAATGCG403.4562618E-739.3751
ACGGTCT2300.039.13043630
TGTTACG750.039.01
TACGGGA3350.038.9552234
TAGGGAT11100.038.9189225
TAAGGGA9850.038.832494
CTAGGGA5450.038.807344
TAGGGAC8650.038.7572255
Produced by FastQC (version 0.11.5)