##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548071_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	773852
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502072747760554	31.0	31.0	33.0	30.0	34.0
2	31.919412239032788	31.0	31.0	34.0	30.0	34.0
3	32.011444048732834	33.0	31.0	34.0	30.0	34.0
4	35.73091107860418	37.0	35.0	37.0	35.0	37.0
5	35.641379747031735	37.0	35.0	37.0	33.0	37.0
6	35.70727477605537	37.0	35.0	37.0	33.0	37.0
7	36.047668029545704	37.0	35.0	37.0	35.0	37.0
8	36.06545308405225	37.0	35.0	37.0	35.0	37.0
9	37.88309909388359	39.0	38.0	39.0	35.0	39.0
10	37.328738570165875	39.0	37.0	39.0	34.0	39.0
11	36.98205470813541	39.0	37.0	39.0	33.0	39.0
12	36.33217462770659	38.0	35.0	39.0	32.0	39.0
13	36.07758977168761	38.0	35.0	39.0	32.0	39.0
14	37.07529088249433	39.0	35.0	41.0	32.0	41.0
15	37.297126582343914	39.0	35.0	41.0	32.0	41.0
16	37.41947814310747	39.0	35.0	41.0	33.0	41.0
17	37.372210706956885	39.0	35.0	41.0	32.0	41.0
18	37.2963835462078	39.0	35.0	41.0	32.0	41.0
19	37.25441686524038	39.0	35.0	41.0	32.0	41.0
20	37.137218744669525	39.0	35.0	41.0	32.0	41.0
21	37.01270527180908	39.0	35.0	41.0	32.0	41.0
22	36.92797201532076	39.0	35.0	41.0	32.0	41.0
23	36.85272894558649	38.0	35.0	41.0	31.0	41.0
24	36.862409091144045	38.0	35.0	41.0	32.0	41.0
25	36.81839421491448	38.0	35.0	41.0	31.0	41.0
26	36.68788605573159	38.0	35.0	41.0	31.0	41.0
27	36.61806521143578	38.0	35.0	41.0	31.0	41.0
28	36.555395605361234	38.0	35.0	41.0	31.0	41.0
29	36.499981908685385	38.0	35.0	40.0	31.0	41.0
30	36.43792223836083	38.0	35.0	40.0	31.0	41.0
31	36.36672774639078	38.0	35.0	40.0	30.0	41.0
32	36.25128319110114	38.0	35.0	41.0	30.0	41.0
33	36.141299628352705	38.0	35.0	41.0	30.0	41.0
34	36.02132449098794	38.0	35.0	41.0	30.0	41.0
35	35.90532298165541	38.0	35.0	40.0	30.0	41.0
36	35.749426246879246	38.0	35.0	40.0	29.0	41.0
37	35.64518667652212	38.0	35.0	40.0	28.0	41.0
38	35.5398525816306	38.0	34.0	40.0	28.0	41.0
39	35.44828468492683	38.0	34.0	40.0	27.0	41.0
40	35.288353586990794	38.0	34.0	40.0	27.0	41.0
41	35.22199722944439	38.0	34.0	40.0	26.0	41.0
42	35.16690659195815	38.0	34.0	40.0	26.0	41.0
43	35.05239115489783	37.0	34.0	40.0	26.0	41.0
44	34.89632642934308	37.0	34.0	40.0	26.0	41.0
45	34.86311077570388	37.0	34.0	40.0	26.0	41.0
46	34.80179414151543	37.0	34.0	40.0	25.0	41.0
47	34.70742596775611	37.0	34.0	40.0	25.0	41.0
48	34.576960452386245	37.0	34.0	40.0	24.0	41.0
49	34.472698914004226	36.0	34.0	40.0	24.0	41.0
50	34.29357810020521	36.0	33.0	40.0	24.0	41.0
51	33.93023601412156	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	5.0
14	11.0
15	28.0
16	62.0
17	154.0
18	336.0
19	663.0
20	1125.0
21	1689.0
22	2562.0
23	3904.0
24	5529.0
25	7878.0
26	10216.0
27	12221.0
28	13494.0
29	15747.0
30	18616.0
31	22602.0
32	27965.0
33	36554.0
34	62921.0
35	94536.0
36	52844.0
37	70028.0
38	110500.0
39	201629.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9627629055685	24.638302931309862	29.734238588257185	12.664695574864446
2	29.647529501765195	25.898492218150242	30.838196451000965	13.615781829083598
3	29.58278844016685	25.50177553330611	30.8980270129172	14.01740901360984
4	26.261869194626364	28.259667223189965	30.270646066689753	15.207817515493918
5	23.616402102727655	32.452717057008314	28.888469629851706	15.042411210412327
6	21.792280694499723	41.90413670831115	25.701167665134932	10.602414932054192
7	89.66197670872467	3.4264949887058505	5.16132283692489	1.7502054656445936
8	90.19644583201955	2.83284142187395	5.063629737986075	1.9070830081204158
9	85.60422406351603	5.269095382579614	6.5657257460082805	2.5609548078960835
10	45.204380165716444	33.46130784697849	11.676780573029468	9.657531414275597
11	36.90990008425384	21.116053198802874	25.450344510319802	16.52370220662349
12	34.59072277386374	20.859156531223025	27.882592537074274	16.66752815783897
13	25.051172575634617	31.086822803326736	26.797372107328016	17.06463251371063
14	18.426650057116863	35.08552022867421	29.036043067666682	17.45178664654223
15	17.253686751471857	26.13807291316686	40.21001431798329	16.398226017378
16	20.868977530587244	23.827165918030836	38.17319073931449	17.130665812067424
17	21.428386823320224	23.141505093997303	27.738508138507108	27.691599944175373
18	22.739748685795217	25.300445046339608	31.636411096695493	20.323395171169683
19	26.597850751823348	26.21728702645984	26.810940593291743	20.373921628425073
20	28.06285956487804	25.74536216227392	26.088967916345762	20.102810356502275
21	24.630807958110854	26.455963155745543	29.30844657634793	19.60478230979567
22	24.16392281728289	22.69994779363496	28.941968231651533	24.194161157430617
23	20.69103652894869	27.534076283320324	28.039599303225938	23.735287884505045
24	21.041878808867846	25.74394070183963	33.25429151827481	19.959888971017715
25	20.48608777905853	26.244036327359755	31.45601484521588	21.813861048365837
26	20.07076288489272	31.550606575934413	26.81042369858836	21.568206840584505
27	19.81670913818146	30.391987098308203	29.828959542651567	19.962344220858768
28	17.959894139964746	27.837881145231908	34.91106826628347	19.29115644851987
29	18.69776132903966	25.031659800582023	34.50659816088864	21.76398070948967
30	20.304786961848002	27.094198890743964	31.505249065712825	21.09576508169521
31	25.93467484738684	25.813204592092543	26.87335562872487	21.378764931795743
32	25.888412771434332	26.790006357804852	27.60514930503507	19.716431565725745
33	24.892485901696965	26.509720204897057	27.77365697833694	20.82413691506903
34	20.89520993678378	27.20817417283925	28.59526110935941	23.301354781017558
35	20.552250301091167	26.611677685138762	30.41382589952601	22.422246114244068
36	26.53918320298972	25.332492517949166	28.02706460666898	20.10125967239214
37	21.605294035552017	28.668660157239366	29.129988680005997	20.59605712720262
38	21.854437282581166	29.367243348857404	26.001612711474547	22.776706657086883
39	21.142414828675253	28.200353555977113	28.496534221013835	22.160697394333802
40	23.374495381545827	26.264712115494955	27.948367388079376	22.41242511487985
41	20.351049037800507	24.917813742162583	28.981123005432565	25.750014214604345
42	22.211094627913347	26.18523955485028	27.145759137406117	24.457906679830252
43	22.02811390291684	26.344701570843004	28.177351741676702	23.44983278456346
44	21.033608493613766	28.805766477310907	28.458800907667097	21.70182412140823
45	20.01674738838951	30.978921033996166	26.28771392979536	22.716617647818964
46	22.47484015031298	28.84440435638856	27.74522776965104	20.935527723647414
47	22.118182804980798	27.649214578498217	27.871091629924067	22.36151098659692
48	22.67229392700413	26.074882535678658	29.266836552725845	21.985986984591367
49	22.15449465789324	24.540868279722737	30.60016644009449	22.704470622289534
50	20.647488150188924	28.89893674759515	28.292877707882123	22.160697394333802
51	20.098029080496012	30.05316262024263	26.998444146942823	22.85036415231853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	661.5
2	1005.0
3	3028.0
4	5051.0
5	3760.0
6	2469.0
7	2372.0
8	2275.0
9	2335.5
10	2396.0
11	2412.0
12	2428.0
13	2427.0
14	2426.0
15	2319.5
16	2213.0
17	2108.5
18	2004.0
19	2076.0
20	2148.0
21	2456.0
22	2764.0
23	3405.5
24	4047.0
25	4883.5
26	6488.0
27	7256.0
28	9217.5
29	11179.0
30	12851.5
31	14524.0
32	16529.0
33	18534.0
34	21014.5
35	23495.0
36	25444.0
37	27393.0
38	30979.0
39	34565.0
40	42125.0
41	49685.0
42	56113.0
43	62541.0
44	63181.5
45	63822.0
46	64887.5
47	65953.0
48	64173.0
49	62393.0
50	61621.5
51	60850.0
52	56705.0
53	52560.0
54	47311.0
55	42062.0
56	37781.0
57	33500.0
58	30933.0
59	28366.0
60	25803.0
61	23240.0
62	20178.0
63	17116.0
64	15202.0
65	13288.0
66	10894.0
67	8500.0
68	7188.0
69	5876.0
70	5011.5
71	4147.0
72	3307.5
73	2468.0
74	1969.0
75	1126.5
76	783.0
77	596.0
78	409.0
79	325.0
80	241.0
81	199.0
82	157.0
83	136.5
84	116.0
85	83.0
86	50.0
87	36.5
88	23.0
89	20.0
90	17.0
91	11.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.80501516571696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49123155578523	19.996624055071223
2	5.302339528472131	2.736539038920077
3	2.0419435996486435	1.5807715666941577
4	1.256318360397592	1.296772573721141
5	0.9343248139986178	1.2055132997470006
6	0.7909751798945832	1.2246675907731253
7	0.6936347301753353	1.2529478312149764
8	0.6278959774215065	1.2962292175883718
9	0.5460503638901822	1.2681754129288274
>10	9.78483447990312	58.745742409512026
>50	0.5058143306877885	7.858645004074455
>100	0.02363148463447726	0.99580809335104
>500	0.0	0.0
>1k	0.0010055950908288195	0.5415639064035493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.3623431870693621	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1370	0.17703643590764123	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.292236758449936E-4	0.0	0.0	0.027266195603293655	0.0
2	1.292236758449936E-4	0.0	0.0	0.10363738802768488	0.0
3	1.292236758449936E-4	0.0	0.0	0.16049580539948208	0.0
4	1.292236758449936E-4	0.0	0.0	0.2527615099528075	0.0
5	1.292236758449936E-4	0.0	0.0	0.46817737758641187	0.0
6	1.292236758449936E-4	0.0	0.0	0.7187420850498545	0.0
7	1.292236758449936E-4	0.0	0.0	0.8731643776846219	0.0
8	1.292236758449936E-4	0.0	0.0	1.2808650749755768	0.0
9	1.292236758449936E-4	0.0	0.0	1.4582891819107529	0.0
10	1.292236758449936E-4	0.0	0.0	1.7494301235895235	0.0
11	1.292236758449936E-4	0.0	0.0	2.0262272372495	0.0
12	1.292236758449936E-4	0.0	0.0	2.2565038276052785	0.0
13	1.292236758449936E-4	0.0	0.0	2.3607873340121883	0.0
14	1.292236758449936E-4	0.0	0.0	2.405240278502866	0.0
15	1.292236758449936E-4	0.0	0.0	2.468818327018603	0.0
16	1.292236758449936E-4	0.0	0.0	2.604761634007536	0.0
17	1.292236758449936E-4	0.0	0.0	2.770943281144198	0.0
18	1.292236758449936E-4	0.0	0.0	2.993208003597587	0.0
19	1.292236758449936E-4	0.0	0.0	3.1024020096866067	0.0
20	1.292236758449936E-4	0.0	0.0	3.2299457777456153	0.0
21	1.292236758449936E-4	0.0	0.0	3.3910877015243224	0.0
22	1.292236758449936E-4	0.0	0.0	3.566444229645979	0.0
23	1.292236758449936E-4	0.0	0.0	3.7498126256700246	0.0
24	1.292236758449936E-4	0.0	0.0	3.884463695900508	0.0
25	1.292236758449936E-4	0.0	0.0	4.002703359298677	0.0
26	1.292236758449936E-4	0.0	0.0	4.124044390917127	0.0
27	1.292236758449936E-4	0.0	0.0	4.25223427735536	0.0
28	1.292236758449936E-4	0.0	0.0	4.383396308338028	0.0
29	1.292236758449936E-4	0.0	0.0	4.522311759871396	0.0
30	2.584473516899872E-4	0.0	0.0	4.696763722262138	0.0
31	2.584473516899872E-4	0.0	0.0	4.855321172523945	0.0
32	2.584473516899872E-4	0.0	0.0	4.997984110656818	0.0
33	2.584473516899872E-4	0.0	0.0	5.146979008906095	0.0
34	2.584473516899872E-4	0.0	0.0	5.300238288458258	0.0
35	2.584473516899872E-4	0.0	0.0	5.484769697564909	0.0
36	2.584473516899872E-4	0.0	0.0	5.650692897349881	0.0
37	2.584473516899872E-4	0.0	0.0	5.813773176266263	0.0
38	2.584473516899872E-4	0.0	0.0	6.004507321813473	0.0
39	2.584473516899872E-4	0.0	0.0	6.27329256757106	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	25	3.8896193E-5	45.000004	1
TAATCGG	30	2.1645792E-6	45.000004	2
TCGTTAG	20	7.031857E-4	45.0	1
CATACGA	20	7.031857E-4	45.0	18
TCACGAC	215	0.0	41.860466	25
CGACGGT	220	0.0	40.909092	28
CGGTCTA	220	0.0	40.909092	31
TAGGGCG	275	0.0	40.090904	5
GGGCGAT	935	0.0	39.94652	7
CGTTTTT	1730	0.0	39.797688	1
GCGTAGG	210	0.0	39.642857	2
GGCGATA	200	0.0	39.375004	8
TAATGCG	40	3.4562618E-7	39.375	1
ACGGTCT	230	0.0	39.130436	30
TGTTACG	75	0.0	39.0	1
TACGGGA	335	0.0	38.955223	4
TAGGGAT	1110	0.0	38.918922	5
TAAGGGA	985	0.0	38.83249	4
CTAGGGA	545	0.0	38.80734	4
TAGGGAC	865	0.0	38.757225	5
>>END_MODULE