FastQCFastQC Report
Thu 26 May 2016
SRR1548070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548070_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences676761
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60490.8938162807845015No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7270.10742344786416475No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT253.8890983E-545.00000412
TAACGCC253.8890983E-545.00000412
TAGCGTC207.031228E-445.020
ACGGGCC207.031228E-445.05
AGACGCG207.031228E-445.01
ACGTGGT207.031228E-445.041
TATAGCG207.031228E-445.01
CGACGGT1200.044.99999628
CGGTCTA1200.044.99999631
TCACGAC1300.043.2692325
CTCACGA1300.043.2692324
CGTTTTT33500.042.9850731
CGTAAGG1300.041.538462
ACGGTCT1300.041.5384630
ACGGGAT3750.041.3999985
TCTCACG1400.040.17857423
TACGGGA2600.039.807694
TTACGGG2150.039.7674453
TTAGCGG2000.039.3750042
ATTAGCG800.039.3751
Produced by FastQC (version 0.11.5)