##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548070_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	676761
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521368695891162	31.0	31.0	33.0	30.0	34.0
2	31.920171227360914	31.0	31.0	34.0	30.0	34.0
3	31.889121861336573	33.0	31.0	34.0	30.0	34.0
4	35.67096803746079	37.0	35.0	37.0	33.0	37.0
5	35.627729730288834	37.0	35.0	37.0	33.0	37.0
6	35.715704657922075	37.0	35.0	37.0	33.0	37.0
7	36.066700061025976	37.0	35.0	37.0	35.0	37.0
8	36.09728101944409	37.0	35.0	37.0	35.0	37.0
9	37.91717755603529	39.0	38.0	39.0	35.0	39.0
10	37.37376562774746	39.0	37.0	39.0	35.0	39.0
11	37.05588383491366	39.0	37.0	39.0	33.0	39.0
12	36.57821151041505	39.0	35.0	39.0	33.0	39.0
13	36.40774512715715	39.0	35.0	39.0	32.0	39.0
14	37.491570879527636	40.0	35.0	41.0	32.0	41.0
15	37.65357046283695	40.0	35.0	41.0	33.0	41.0
16	37.739049679281166	40.0	35.0	41.0	33.0	41.0
17	37.6750566300363	40.0	35.0	41.0	33.0	41.0
18	37.5439734263647	39.0	36.0	41.0	33.0	41.0
19	37.460302824778616	39.0	36.0	41.0	32.0	41.0
20	37.312797279985105	39.0	35.0	41.0	32.0	41.0
21	37.191166453149634	39.0	35.0	41.0	32.0	41.0
22	37.11178096846597	39.0	35.0	41.0	32.0	41.0
23	37.01815559702761	39.0	35.0	41.0	32.0	41.0
24	37.01595097826264	39.0	35.0	41.0	32.0	41.0
25	36.99548585098728	39.0	35.0	41.0	32.0	41.0
26	36.87359200663159	39.0	35.0	41.0	32.0	41.0
27	36.81414709180937	39.0	35.0	41.0	31.0	41.0
28	36.739049679281166	38.0	35.0	41.0	31.0	41.0
29	36.66647900809887	38.0	35.0	41.0	31.0	41.0
30	36.57483070094169	38.0	35.0	41.0	31.0	41.0
31	36.46773676379106	38.0	35.0	41.0	30.0	41.0
32	36.29279021693035	38.0	35.0	41.0	30.0	41.0
33	36.114405233162074	38.0	35.0	41.0	30.0	41.0
34	35.930427433022885	38.0	35.0	41.0	29.0	41.0
35	35.75256405141549	38.0	35.0	41.0	28.0	41.0
36	35.572651201827526	38.0	35.0	41.0	27.0	41.0
37	35.47654785071835	38.0	35.0	40.0	26.0	41.0
38	35.366773499063925	38.0	34.0	40.0	26.0	41.0
39	35.32113700405313	38.0	35.0	40.0	26.0	41.0
40	35.190745329591984	38.0	34.0	40.0	25.0	41.0
41	35.12235190857629	38.0	34.0	40.0	24.0	41.0
42	35.06869337919886	38.0	34.0	40.0	24.0	41.0
43	34.95869738356672	38.0	34.0	40.0	24.0	41.0
44	34.793275617241534	38.0	34.0	40.0	24.0	41.0
45	34.76458158788701	38.0	34.0	40.0	24.0	41.0
46	34.68919899344082	37.0	34.0	40.0	23.0	41.0
47	34.62296734002107	37.0	34.0	40.0	23.0	41.0
48	34.510007225593675	37.0	34.0	40.0	23.0	41.0
49	34.406412308037844	37.0	34.0	40.0	23.0	41.0
50	34.250918714287614	37.0	33.0	40.0	23.0	41.0
51	33.8814322929365	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	6.0
15	17.0
16	51.0
17	133.0
18	253.0
19	508.0
20	928.0
21	1545.0
22	2359.0
23	3545.0
24	5495.0
25	8176.0
26	10805.0
27	12587.0
28	12882.0
29	13708.0
30	15569.0
31	18376.0
32	22989.0
33	28894.0
34	48190.0
35	72100.0
36	46482.0
37	61571.0
38	98794.0
39	190770.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.884372769707475	24.0550209010271	28.701860775074213	15.358745554191215
2	32.432867733217485	25.096156545663835	29.752453229426635	12.718522491692044
3	28.838245702692678	25.11817318078317	33.033522912815606	13.010058203708546
4	25.05832930680107	27.49862950140448	32.6261412817819	14.816899910012546
5	22.883115309540592	31.672628889667102	31.22756778242245	14.216688018369853
6	20.79035287198878	41.10963841001476	27.597926003419225	10.502082714577229
7	87.70570408164772	3.128874152027082	7.709516358064368	1.4559054082608187
8	88.69009886799032	2.183193180458094	7.595739116172474	1.5309688353791073
9	83.84082416096672	5.116281818840034	8.800152491056666	2.2427415291365786
10	39.733672596381886	37.249634656843405	14.039520598852476	8.977172147922236
11	31.19683315084646	21.937286575319796	29.985622693979114	16.880257579854632
12	30.32414692927045	21.79912849587964	30.776743931757295	17.099980643092614
13	23.92587634334721	28.776185388933463	30.23371618636417	17.064222081355158
14	17.79742035962474	33.004118145105885	31.305438699925087	17.893022795344294
15	16.882030731676323	27.34983251103418	39.8072583969821	15.960878360307406
16	20.956881380576007	24.898154592241575	37.736364831897816	16.408599195284598
17	20.81828001318043	24.133926157092386	30.433491291608117	24.61430253811907
18	21.874191922997927	25.764191494486237	33.449327015002346	18.912289567513493
19	23.982026151034116	26.767499900260212	29.630253516381703	19.62022043232397
20	25.400399845735794	26.642344934179125	29.2015940634877	18.75566115659738
21	23.286064061020063	26.078630417532928	32.19142356016378	18.44388196128323
22	23.11820568856657	23.15086123461606	31.502997365391916	22.227935711425452
23	19.3332653625135	27.87069585865616	31.28179667563586	21.51424210319448
24	19.268840846325364	26.40296943825073	35.283652574542565	19.044537140881346
25	19.548555546197257	27.448981250397114	32.885021447748905	20.117441755656724
26	19.477924998633195	31.06222137504969	28.859523524552984	20.60033010176414
27	18.887169916706192	29.730584356959106	31.925450786910005	19.4567949394247
28	17.19218453782059	27.928914343468374	35.940900849783006	18.938000268928025
29	18.343551120705833	26.1225159251198	34.974680869612754	20.55925208456161
30	19.931704102334503	27.382340294431863	33.15454052464607	19.531415078587568
31	23.736592386381602	26.81803472717843	29.019993764416093	20.425379122023877
32	23.5994686455041	27.783515894089643	29.510713531069317	19.106301929336944
33	22.245371704338755	28.0664813723013	29.271042509837297	20.417104413522647
34	19.5127969844598	28.256504142525944	30.95967409469517	21.27102477831908
35	19.4163079728294	28.32935112986712	30.834075840658663	21.420265056644812
36	23.7481178732226	26.979096017648775	29.879676872633027	19.3931092364956
37	20.445474842669718	29.572773844828532	30.02743952444068	19.954311788061073
38	20.5744716376978	30.251595467232896	28.172574956299197	21.001357938770113
39	19.954164025409266	29.319065371674785	29.20558365508651	21.52118694782944
40	21.72465611936858	27.873946636995928	28.973744054400296	21.427653189235198
41	19.402122758255867	26.85822616847011	29.80638659733643	23.93326447593759
42	21.28757419532154	28.21646046388607	27.995555299433626	22.500410041358766
43	21.37623178640613	28.37456650132026	27.92448146391414	22.324720248359466
44	20.534427959057926	29.834461501179888	28.70895338236098	20.92215715740121
45	19.588303699533512	31.24795902837191	27.536900028222668	21.626837243871915
46	21.737659232727655	30.025223084663565	28.447856776616852	19.789260905991924
47	21.045391209008795	28.60684938996189	29.081610790219887	21.26614861080943
48	21.568027708452465	27.584479602104732	29.99921685794542	20.848275831497382
49	20.623085550142516	26.66672577172739	30.239478929784664	22.470709748345428
50	19.54249727747314	29.18578345974428	29.899329305323448	21.37238995745913
51	19.58859922483713	30.412361232399622	28.416087806478206	21.58295173628504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	633.5
2	939.0
3	5450.0
4	9961.0
5	7237.5
6	4514.0
7	4214.0
8	3914.0
9	3997.0
10	4080.0
11	4148.5
12	4217.0
13	4009.0
14	3801.0
15	3616.0
16	3431.0
17	3272.0
18	3113.0
19	3174.5
20	3236.0
21	3408.5
22	3581.0
23	4025.0
24	4469.0
25	5127.5
26	6604.5
27	7423.0
28	9078.5
29	10734.0
30	12685.0
31	14636.0
32	17032.5
33	19429.0
34	21860.0
35	24291.0
36	25177.0
37	26063.0
38	29232.5
39	32402.0
40	37846.5
41	43291.0
42	49050.0
43	54809.0
44	56550.0
45	58291.0
46	58485.5
47	58680.0
48	55875.5
49	53071.0
50	49851.5
51	46632.0
52	42859.0
53	39086.0
54	35998.0
55	32910.0
56	29475.0
57	26040.0
58	23388.0
59	20736.0
60	18841.5
61	16947.0
62	15008.5
63	13070.0
64	10784.5
65	8499.0
66	6865.0
67	5231.0
68	4409.0
69	3587.0
70	2934.0
71	2281.0
72	1852.5
73	1424.0
74	1170.0
75	654.5
76	393.0
77	307.0
78	221.0
79	192.5
80	164.0
81	106.0
82	48.0
83	46.5
84	45.0
85	30.0
86	15.0
87	13.0
88	11.0
89	10.0
90	9.0
91	5.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	676761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.57838959211079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78798299356481	22.5631769119327
2	6.195737674185076	3.7891136082349925
3	2.902227287182979	2.662363100170078
4	2.036436738803955	2.4908382391533954
5	1.6042075759389354	2.4527042121838205
6	1.309497365405461	2.4025392365526503
7	1.1164091859043177	2.3896596521555424
8	1.0143391505195873	2.481348617849492
9	0.9037623369344358	2.487203715371191
>10	8.980161446195565	51.94057500217374
>50	0.13373297311971155	2.4946449259946823
>100	0.014051652972186206	0.7630469423561221
>500	9.690795153231865E-4	0.18653904862647724
>1k	0.0	0.0
>5k	4.8453975766159327E-4	0.8962467872450841
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6049	0.8938162807845015	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	727	0.10742344786416475	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.477626518076544E-4	0.0	0.0	0.03191673279045335	0.0
2	1.477626518076544E-4	0.0	0.0	0.09988755262197438	0.0
3	1.477626518076544E-4	0.0	0.0	0.16830166040891836	0.0
4	1.477626518076544E-4	0.0	0.0	0.24247851161636086	0.0
5	1.477626518076544E-4	0.0	0.0	0.44210585420850196	0.0
6	1.477626518076544E-4	0.0	0.0	0.7541805748262681	0.0
7	1.477626518076544E-4	0.0	0.0	0.9613438126605995	0.0
8	1.477626518076544E-4	0.0	0.0	1.3997556005739102	0.0
9	1.477626518076544E-4	0.0	0.0	1.6119427685697019	0.0
10	1.477626518076544E-4	0.0	0.0	1.8965336359512441	0.0
11	1.477626518076544E-4	0.0	0.0	2.1468435681134106	0.0
12	1.477626518076544E-4	0.0	0.0	2.34972168904532	0.0
13	1.477626518076544E-4	0.0	0.0	2.4410390078624506	0.0
14	1.477626518076544E-4	0.0	0.0	2.486254379315593	0.0
15	1.477626518076544E-4	0.0	0.0	2.541369848439848	0.0
16	1.477626518076544E-4	0.0	0.0	2.6570680048052413	0.0
17	1.477626518076544E-4	0.0	0.0	2.8049784192647036	0.0
18	1.477626518076544E-4	0.0	0.0	2.979633873701351	0.0
19	1.477626518076544E-4	0.0	0.0	3.0777482745016336	0.0
20	1.477626518076544E-4	0.0	0.0	3.19388971882245	0.0
21	1.477626518076544E-4	0.0	0.0	3.331013459699953	0.0
22	1.477626518076544E-4	0.0	0.0	3.486607532053413	0.0
23	1.477626518076544E-4	0.0	0.0	3.653579328596063	0.0
24	1.477626518076544E-4	0.0	0.0	3.783462699534991	0.0
25	1.477626518076544E-4	0.0	0.0	3.8931025871762706	0.0
26	1.477626518076544E-4	0.0	0.0	3.9965364434416286	0.0
27	1.477626518076544E-4	0.0	0.0	4.111939074503407	0.0
28	1.477626518076544E-4	0.0	0.0	4.2433000719604115	0.0
29	1.477626518076544E-4	0.0	0.0	4.3793894742752615	0.0
30	1.477626518076544E-4	0.0	0.0	4.546065745514295	0.0
31	1.477626518076544E-4	0.0	0.0	4.688508941856874	0.0
32	1.477626518076544E-4	0.0	0.0	4.824893869475339	0.0
33	1.477626518076544E-4	0.0	0.0	4.9660072019516495	0.0
34	1.477626518076544E-4	0.0	0.0	5.111257888678574	0.0
35	1.477626518076544E-4	0.0	0.0	5.29418805161645	0.0
36	1.477626518076544E-4	0.0	0.0	5.4447581938084495	0.0
37	1.477626518076544E-4	0.0	0.0	5.607740398752292	0.0
38	1.477626518076544E-4	0.0	0.0	5.781657039929901	0.0
39	1.477626518076544E-4	0.0	0.0	6.000789052560653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCT	25	3.8890983E-5	45.000004	12
TAACGCC	25	3.8890983E-5	45.000004	12
TAGCGTC	20	7.031228E-4	45.0	20
ACGGGCC	20	7.031228E-4	45.0	5
AGACGCG	20	7.031228E-4	45.0	1
ACGTGGT	20	7.031228E-4	45.0	41
TATAGCG	20	7.031228E-4	45.0	1
CGACGGT	120	0.0	44.999996	28
CGGTCTA	120	0.0	44.999996	31
TCACGAC	130	0.0	43.26923	25
CTCACGA	130	0.0	43.26923	24
CGTTTTT	3350	0.0	42.985073	1
CGTAAGG	130	0.0	41.53846	2
ACGGTCT	130	0.0	41.53846	30
ACGGGAT	375	0.0	41.399998	5
TCTCACG	140	0.0	40.178574	23
TACGGGA	260	0.0	39.80769	4
TTACGGG	215	0.0	39.767445	3
TTAGCGG	200	0.0	39.375004	2
ATTAGCG	80	0.0	39.375	1
>>END_MODULE