FastQCFastQC Report
Thu 26 May 2016
SRR1548069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548069_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2236380
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64710.28935154133018537No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT45630.204035092426153No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41350.18489702107870756No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC253.8919927E-545.00000429
ACGGTAC253.8919927E-545.00000413
CGAATCG207.034719E-445.015
CGACGGT2350.044.04255328
TATAGCG1100.042.9545441
GCTACGA5700.042.23684310
CGTTAGG3450.041.0869562
TCGTTAG1050.040.7142871
TACGCGA501.0822987E-940.50000441
TGATTCG501.0822987E-940.50000415
GTATACG900.040.01
ACGTTAG850.039.7058831
CGTAAGG3750.039.6000022
TCACGAC2900.039.56896625
ACGTAAG1600.039.3751
CATACGA7550.039.3377518
GACCGAT6400.038.6718759
ATACGAG1050.038.571431
GTATTCG356.2509407E-638.57142640
GCGATGA3050.038.3606579
Produced by FastQC (version 0.11.5)