##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548069_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2236380
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53034904622649	31.0	31.0	33.0	30.0	34.0
2	31.933784508893837	31.0	31.0	34.0	30.0	34.0
3	32.022750605889875	33.0	31.0	34.0	30.0	34.0
4	35.73481742816516	37.0	35.0	37.0	35.0	37.0
5	35.645191335998355	37.0	35.0	37.0	33.0	37.0
6	35.71074414902655	37.0	35.0	37.0	33.0	37.0
7	36.03423881451274	37.0	35.0	37.0	35.0	37.0
8	36.042192740053125	37.0	35.0	37.0	35.0	37.0
9	37.80133921784312	39.0	38.0	39.0	35.0	39.0
10	37.336758064371885	39.0	37.0	39.0	34.0	39.0
11	36.99947370303794	39.0	37.0	39.0	33.0	39.0
12	36.28296219783758	38.0	35.0	39.0	32.0	39.0
13	35.975134368935514	38.0	35.0	39.0	32.0	39.0
14	37.021774027669714	39.0	35.0	41.0	32.0	41.0
15	37.21843425535911	39.0	35.0	41.0	32.0	41.0
16	37.37100045609422	39.0	35.0	41.0	33.0	41.0
17	37.30196388806911	39.0	35.0	41.0	32.0	41.0
18	37.21527110777238	39.0	35.0	41.0	32.0	41.0
19	37.15614609323997	39.0	35.0	41.0	32.0	41.0
20	37.04155599674474	39.0	35.0	41.0	32.0	41.0
21	36.90679088526994	38.0	35.0	41.0	32.0	41.0
22	36.79539389549182	38.0	35.0	41.0	31.0	41.0
23	36.72521530330266	38.0	35.0	41.0	31.0	41.0
24	36.73618794659226	38.0	35.0	41.0	31.0	41.0
25	36.70523971775816	38.0	35.0	41.0	31.0	41.0
26	36.55999651222064	38.0	35.0	41.0	31.0	41.0
27	36.47977222117887	38.0	35.0	40.0	31.0	41.0
28	36.40149929797261	38.0	35.0	40.0	31.0	41.0
29	36.37587485132222	38.0	35.0	40.0	31.0	41.0
30	36.31830234575519	38.0	35.0	40.0	31.0	41.0
31	36.22263747663635	38.0	35.0	40.0	30.0	41.0
32	36.06743084806696	38.0	35.0	40.0	30.0	41.0
33	35.97666049598011	38.0	35.0	40.0	30.0	41.0
34	35.834046092345666	38.0	35.0	40.0	30.0	41.0
35	35.72359616880852	38.0	35.0	40.0	29.0	41.0
36	35.537091192015666	38.0	35.0	40.0	28.0	41.0
37	35.422327600854956	38.0	34.0	40.0	27.0	41.0
38	35.33449726790617	38.0	34.0	40.0	27.0	41.0
39	35.30608841073521	37.0	34.0	40.0	27.0	41.0
40	35.1204321269194	37.0	34.0	40.0	26.0	41.0
41	35.07311056260564	37.0	34.0	40.0	26.0	41.0
42	35.00973001010562	37.0	34.0	40.0	26.0	41.0
43	34.89443833337805	37.0	34.0	40.0	26.0	41.0
44	34.68556998363427	37.0	34.0	40.0	25.0	41.0
45	34.643024441284574	36.0	34.0	40.0	25.0	41.0
46	34.54031917652635	36.0	34.0	40.0	24.0	41.0
47	34.464764485463114	36.0	33.0	40.0	24.0	41.0
48	34.35733104391919	36.0	33.0	40.0	24.0	41.0
49	34.23733086505871	36.0	33.0	40.0	24.0	41.0
50	34.0820111072358	35.0	33.0	40.0	24.0	41.0
51	33.70612641858718	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	10.0
12	7.0
13	21.0
14	37.0
15	87.0
16	197.0
17	453.0
18	971.0
19	1792.0
20	3347.0
21	5208.0
22	8160.0
23	11818.0
24	16844.0
25	23404.0
26	30170.0
27	35869.0
28	40404.0
29	46347.0
30	54837.0
31	66943.0
32	82316.0
33	109066.0
34	193311.0
35	300499.0
36	150187.0
37	193882.0
38	300686.0
39	559362.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.58942576842934	23.447491034618444	29.11025854282367	12.852824654128547
2	30.52558152013522	26.44358293313301	29.493243545372433	13.53759200135934
3	30.069442581314448	25.294940931326522	30.49414679079584	14.141469696563197
4	27.499396345880395	27.650086300181542	29.35203319650507	15.498484157432996
5	23.844471869718024	32.80466646992014	28.058603636233553	15.292258024128277
6	22.52931076114077	41.20708466360815	25.092381437859395	11.171223137391678
7	87.4234700721702	4.481975335139824	5.89041218397589	2.2041424087140826
8	87.55698942040262	4.1739328736619	5.7610513419007505	2.5080263640347344
9	81.89994544755363	6.861848165338627	7.532843255618454	3.705363131489282
10	46.87830332948783	29.386329693522566	12.341641402623884	11.393725574365716
11	40.54498788220249	21.61671987765943	22.385015068995433	15.453277171142652
12	38.094107441490266	20.74307586367254	25.31980253803021	15.843014156806984
13	23.567416986379776	34.37349645409098	25.031613589819262	17.02747296970998
14	17.372628980763555	37.489246013647055	26.811454225131687	18.326670780457704
15	15.86358311199349	25.79275436195995	42.08640749783132	16.25725502821524
16	18.49471020130747	21.7661578086014	40.59260948497125	19.146522505119883
17	19.130648637530296	21.34368935511854	28.223870719645138	31.301791287706028
18	21.80040959049893	25.73672631663671	31.242051887425216	21.220812205439145
19	27.580867294466948	25.787790983643212	25.807733927150128	20.82360779473971
20	29.549718741895386	24.02445022759996	26.013065758055433	20.412765272449228
21	23.837630456362515	26.98101396006046	27.63980182258829	21.54155376098874
22	23.034815192409162	24.881549647197705	26.156735438521185	25.926899721871955
23	21.38111591053399	28.93390210965936	26.272860605085	23.412121374721647
24	21.06766291953961	24.5461862474177	34.027177849918175	20.358972983124513
25	20.393448340621898	26.47296076695374	30.9426841592216	22.190906733202766
26	19.420715620780012	32.24577218540677	26.55438700042032	21.779125193392893
27	20.495801250234756	30.891306486375303	28.681529972544915	19.931362290845026
28	17.511871864352212	28.46323075684812	35.19218558563393	18.83271179316574
29	18.98738139314428	25.698271313461934	33.54014076319767	21.77420653019612
30	20.354948622327154	28.417889625197862	30.24883964263676	20.97832210983822
31	27.047550058576807	27.559940618320677	24.60118584498162	20.79132347812089
32	27.296657991933394	27.559269891521122	26.351693361593288	18.7923787549522
33	25.598780171527203	28.334048775252864	25.959273468730714	20.10789758448922
34	20.62780028438816	28.149688335613803	28.917536375750096	22.304975004247936
35	21.925164775217095	25.94957028769708	29.741591321689516	22.38367361539631
36	26.83586868063567	26.446757706650924	26.659735823071212	20.05763778964219
37	22.102504941020758	30.15297042541965	28.397141809531472	19.347382824028116
38	21.52147667212191	29.621531224568276	25.955204392813386	22.90178771049643
39	22.413766891136568	28.42406031175382	27.16228905642154	21.999883740688077
40	23.87966266913494	26.484318407426287	27.31217413856321	22.323844784875558
41	18.815496471977035	25.751348160867117	28.62912385193929	26.804031515216554
42	22.649996869941603	26.57146817624912	25.65981631028716	25.11871864352212
43	23.417889625197866	26.867705846054786	26.49920854237652	23.215195986370833
44	21.65141881075667	29.59886065874315	27.17243938865488	21.577281141845305
45	20.247185183197846	32.787093427771666	23.862536778186175	23.10318461084431
46	21.945510154803745	30.88433092765988	26.090735921444473	21.0794229960919
47	23.12004221107325	27.23432511469428	27.748012412917305	21.89762026131516
48	23.486885055312605	24.0088446507302	29.849041754979027	22.65522853897817
49	20.947781682898256	25.04167449181266	30.666076427083055	23.344467398206028
50	19.043722444307317	29.86084654665129	28.618660513866157	22.476770495175238
51	19.876943989840726	29.76873339951171	26.213344780404046	24.140977830243518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1085.0
1	2285.5
2	3486.0
3	8148.0
4	12810.0
5	10038.5
6	7267.0
7	6970.5
8	6674.0
9	7074.0
10	7474.0
11	7205.5
12	6937.0
13	6925.0
14	6913.0
15	6568.5
16	6224.0
17	6385.5
18	6547.0
19	6400.0
20	6253.0
21	6980.0
22	7707.0
23	8198.5
24	8690.0
25	10679.0
26	16346.0
27	20024.0
28	24137.0
29	28250.0
30	34922.0
31	41594.0
32	47063.5
33	52533.0
34	60171.0
35	67809.0
36	68712.5
37	69616.0
38	80213.0
39	90810.0
40	112645.0
41	134480.0
42	163002.0
43	191524.0
44	194572.0
45	197620.0
46	197385.5
47	197151.0
48	189914.5
49	182678.0
50	174626.0
51	166574.0
52	153388.0
53	140202.0
54	125869.5
55	111537.0
56	104997.0
57	98457.0
58	95359.0
59	92261.0
60	88160.5
61	84060.0
62	70685.5
63	57311.0
64	49314.0
65	41317.0
66	33640.0
67	25963.0
68	21335.5
69	16708.0
70	13745.0
71	10782.0
72	8494.5
73	6207.0
74	5357.0
75	3607.0
76	2707.0
77	2118.5
78	1530.0
79	1122.5
80	715.0
81	546.5
82	378.0
83	269.0
84	160.0
85	124.0
86	88.0
87	61.5
88	35.0
89	33.5
90	32.0
91	21.5
92	11.0
93	9.5
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2236380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.637181014710563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16587188392795	16.95671971300138
2	7.107101489607325	2.9334107986189086
3	2.119028972796749	1.3119235346106808
4	0.998332432960626	0.8241106852745936
5	0.5942271661426424	0.6131586795772099
6	0.3880363704696988	0.4804786090604677
7	0.27595089517581417	0.39863940024402883
8	0.22647788948040154	0.3739092160829328
9	0.19775525459701085	0.3672999887155817
>10	2.930181082094897	15.929865544612646
>50	1.9379603523241449	29.278982389071377
>100	1.0529933113342742	28.926191994552948
>500	0.004127681524326598	0.5234314860299772
>1k	0.0017379711681375152	0.7917601750140812
>5k	2.172463960171894E-4	0.2901177855331891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6471	0.28935154133018537	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4563	0.204035092426153	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4135	0.18489702107870756	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7886047988266752E-4	0.0	0.0	0.040243607973600194	0.0
2	1.7886047988266752E-4	0.0	0.0	0.15914111197560343	0.0
3	1.7886047988266752E-4	0.0	0.0	0.22961214104937444	0.0
4	1.7886047988266752E-4	0.0	0.0	0.3579445353651884	0.0
5	1.7886047988266752E-4	0.0	0.0	0.6375481805417684	0.0
6	1.7886047988266752E-4	0.0	0.0	0.9114730054820737	0.0
7	1.7886047988266752E-4	0.0	0.0	1.0900651946449171	0.0
8	1.7886047988266752E-4	0.0	0.0	1.6112646330230105	0.0
9	1.7886047988266752E-4	0.0	0.0	1.8333199187973421	0.0
10	1.7886047988266752E-4	0.0	0.0	2.2235040556613814	0.0
11	1.7886047988266752E-4	0.0	0.0	2.576708788309679	0.0
12	1.7886047988266752E-4	0.0	0.0	2.887478872105814	0.0
13	1.7886047988266752E-4	0.0	0.0	3.0224738192972573	0.0
14	1.7886047988266752E-4	0.0	0.0	3.0692011196666042	0.0
15	1.7886047988266752E-4	0.0	0.0	3.1577370572085246	0.0
16	1.7886047988266752E-4	0.0	0.0	3.3520242534810722	0.0
17	1.7886047988266752E-4	0.0	0.0	3.5605755730242623	0.0
18	1.7886047988266752E-4	0.0	0.0	3.8506872713939493	0.0
19	2.235755998533344E-4	0.0	0.0	3.98818626530375	0.0
20	2.235755998533344E-4	0.0	0.0	4.146880226079647	0.0
21	2.235755998533344E-4	0.0	0.0	4.333789427557035	0.0
22	2.235755998533344E-4	0.0	0.0	4.5319221241470595	0.0
23	2.235755998533344E-4	0.0	0.0	4.7459286883266705	0.0
24	2.235755998533344E-4	0.0	0.0	4.90278932918377	0.0
25	2.235755998533344E-4	0.0	0.0	5.037784276375214	0.0
26	2.235755998533344E-4	0.0	0.0	5.172913368926569	0.0
27	2.235755998533344E-4	0.0	0.0	5.303168513401121	0.0
28	2.235755998533344E-4	0.0	0.0	5.447419490426493	0.0
29	2.235755998533344E-4	0.0	0.0	5.597975299367728	0.0
30	2.235755998533344E-4	0.0	0.0	5.792173065400334	0.0
31	2.235755998533344E-4	0.0	0.0	5.965488870406639	0.0
32	2.235755998533344E-4	0.0	0.0	6.13312585517667	0.0
33	2.235755998533344E-4	0.0	0.0	6.301970148185908	0.0
34	2.235755998533344E-4	0.0	0.0	6.469651848075908	0.0
35	2.235755998533344E-4	0.0	0.0	6.681869807456693	0.0
36	2.6829071982400126E-4	0.0	0.0	6.8747261198901795	0.0
37	2.6829071982400126E-4	0.0	0.0	7.064765379765514	0.0
38	2.6829071982400126E-4	0.0	0.0	7.249036389164632	0.0
39	2.6829071982400126E-4	0.0	0.0	7.4326366717641905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	25	3.8919927E-5	45.000004	29
ACGGTAC	25	3.8919927E-5	45.000004	13
CGAATCG	20	7.034719E-4	45.0	15
CGACGGT	235	0.0	44.042553	28
TATAGCG	110	0.0	42.954544	1
GCTACGA	570	0.0	42.236843	10
CGTTAGG	345	0.0	41.086956	2
TCGTTAG	105	0.0	40.714287	1
TACGCGA	50	1.0822987E-9	40.500004	41
TGATTCG	50	1.0822987E-9	40.500004	15
GTATACG	90	0.0	40.0	1
ACGTTAG	85	0.0	39.705883	1
CGTAAGG	375	0.0	39.600002	2
TCACGAC	290	0.0	39.568966	25
ACGTAAG	160	0.0	39.375	1
CATACGA	755	0.0	39.33775	18
GACCGAT	640	0.0	38.671875	9
ATACGAG	105	0.0	38.57143	1
GTATTCG	35	6.2509407E-6	38.571426	40
GCGATGA	305	0.0	38.360657	9
>>END_MODULE