FastQCFastQC Report
Thu 26 May 2016
SRR1548068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548068_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1506569
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52650.34946955632300947No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26650.1768919976449801No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT253.8913844E-545.00000414
TACGATA253.8913844E-545.00000436
CGAATTA253.8913844E-545.00000417
TAGTCGG253.8913844E-545.0000042
CATTGCG207.033985E-445.013
GTTACGT207.033985E-445.015
CGCTATA207.033985E-445.029
CGTAACT406.8175723E-945.033
CCGGTTA207.033985E-445.028
ATACGCG351.2122655E-745.01
TACGTAC207.033985E-445.010
AGTCGAC207.033985E-445.038
CGTATCG302.1659525E-644.99999634
CGCGAAC302.1659525E-644.99999642
TACGTCA302.1659525E-644.99999638
TATAGCG1900.042.631581
TCGATTG750.042.01
TTTAGCG1450.041.896551
TCGTCCC1450.041.8965538
CGCCGGT1400.041.78571328