##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548068_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1506569
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535134467787405	31.0	31.0	33.0	30.0	34.0
2	31.942218378315232	31.0	31.0	34.0	30.0	34.0
3	32.03093253611351	33.0	31.0	34.0	30.0	34.0
4	35.73364445969617	37.0	35.0	37.0	35.0	37.0
5	35.638120125928516	37.0	35.0	37.0	33.0	37.0
6	35.70628892536618	37.0	35.0	37.0	33.0	37.0
7	36.074066305625564	37.0	35.0	37.0	35.0	37.0
8	36.106416632759604	37.0	35.0	37.0	35.0	37.0
9	37.93387690839251	39.0	38.0	39.0	35.0	39.0
10	37.36076807633769	39.0	37.0	39.0	35.0	39.0
11	36.95713903578263	39.0	37.0	39.0	33.0	39.0
12	36.1062905183898	37.0	35.0	39.0	32.0	39.0
13	35.751940999715245	37.0	35.0	39.0	31.0	39.0
14	36.74311631262823	39.0	35.0	41.0	31.0	41.0
15	37.034105308153826	39.0	35.0	41.0	32.0	41.0
16	37.21845796641242	39.0	35.0	41.0	33.0	41.0
17	37.145817416925475	38.0	35.0	41.0	32.0	41.0
18	37.082978609011604	38.0	35.0	41.0	32.0	41.0
19	37.033292866108354	38.0	35.0	41.0	32.0	41.0
20	36.91110529952495	38.0	35.0	41.0	32.0	41.0
21	36.77903434890801	38.0	35.0	41.0	32.0	41.0
22	36.668886722081766	38.0	35.0	41.0	31.0	41.0
23	36.62173388673204	38.0	35.0	40.0	31.0	41.0
24	36.61831286851117	38.0	35.0	41.0	31.0	41.0
25	36.580274783299004	38.0	35.0	40.0	31.0	41.0
26	36.45332739489529	38.0	35.0	40.0	31.0	41.0
27	36.3974832881866	38.0	35.0	40.0	31.0	41.0
28	36.36801699756201	38.0	35.0	40.0	31.0	41.0
29	36.352652284760936	38.0	35.0	40.0	31.0	41.0
30	36.31265677177746	38.0	35.0	40.0	31.0	41.0
31	36.192779089440975	38.0	35.0	40.0	31.0	41.0
32	36.062058890100616	38.0	35.0	40.0	30.0	41.0
33	35.95767668125389	38.0	35.0	40.0	30.0	41.0
34	35.88402456176916	38.0	35.0	40.0	30.0	41.0
35	35.79328195389657	38.0	35.0	40.0	30.0	41.0
36	35.598356928889416	37.0	35.0	40.0	29.0	41.0
37	35.49721585934663	37.0	34.0	40.0	29.0	41.0
38	35.43843395158137	37.0	34.0	40.0	29.0	41.0
39	35.451669986572135	37.0	34.0	40.0	29.0	41.0
40	35.289646873126955	37.0	34.0	40.0	28.0	41.0
41	35.289147725726465	37.0	34.0	40.0	28.0	41.0
42	35.2427004670878	37.0	34.0	40.0	28.0	41.0
43	35.114963868233055	37.0	34.0	40.0	27.0	41.0
44	34.95194710630579	36.0	34.0	40.0	27.0	41.0
45	34.92173806841904	36.0	34.0	40.0	27.0	41.0
46	34.834917617447324	36.0	34.0	40.0	27.0	41.0
47	34.76409776120443	36.0	34.0	40.0	27.0	41.0
48	34.68198137622638	36.0	34.0	40.0	26.0	41.0
49	34.61130157330995	36.0	34.0	40.0	26.0	41.0
50	34.438597236502275	36.0	34.0	40.0	26.0	41.0
51	34.083601215742526	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	0.0
13	5.0
14	7.0
15	32.0
16	93.0
17	187.0
18	531.0
19	994.0
20	1891.0
21	3055.0
22	4775.0
23	6937.0
24	9877.0
25	14400.0
26	19024.0
27	22713.0
28	25929.0
29	30160.0
30	36499.0
31	44779.0
32	56637.0
33	74433.0
34	137275.0
35	227591.0
36	94561.0
37	122722.0
38	195198.0
39	376173.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4194909094771	24.148645033848435	31.117990613108326	12.313873443566143
2	28.953735275317626	26.70777110109129	31.231095290026545	13.107398333564543
3	28.935083623783576	25.561391479580426	31.930167154640777	13.573357741995224
4	25.843223908098466	28.55647501043762	30.833835025146545	14.766466056317368
5	23.744348914653095	32.80646289682053	29.643713630109207	13.805474558417172
6	21.408312529993648	42.48381587567513	25.510879355675048	10.59699223865618
7	91.02955125188424	3.34992954189287	4.402055265971887	1.218463940250994
8	92.1231619660301	2.3880087802151775	4.1438526877959125	1.344976565958811
9	88.00267362463983	4.870736089750951	5.26222164401365	1.8643686415955725
10	49.943281721580625	32.210804815444895	10.048593857964686	7.797319605009793
11	41.768614646922906	20.339725561856113	23.11052464241598	14.781135148805
12	39.23895951662353	20.47420330565676	24.411958562800642	15.874878614919064
13	23.052910288211162	36.286290239610665	24.326798175191445	16.33400129698673
14	15.90959325460699	39.63807830905853	27.290684993518383	17.161643442816093
15	14.208708661866797	26.719121394373573	43.86523285690864	15.206937086850983
16	19.0990920429134	21.45225343147244	42.57156492666449	16.877089598949667
17	18.570805585406312	22.05952731006678	27.47740063681119	31.892266467715718
18	22.411054521897107	25.161675303288465	32.87137860927711	19.555891565537323
19	26.848222683461557	25.919888169741977	26.28422594650494	20.947663200291522
20	29.357832266560642	25.0978879825617	26.313962387384848	19.23031736349281
21	23.217987360685104	27.323142849746674	29.133680568231522	20.325189221336693
22	23.88134894584981	23.008372002875408	27.710712220947066	25.399566830327718
23	19.295432203901715	28.85085249995188	26.85990485666438	24.99381043948203
24	21.17944813679294	24.316974529543618	36.27454169042374	18.229035643239705
25	18.377585095671026	26.15067746648179	33.135488650038596	22.33624878780859
26	17.767855305664725	34.48816483015381	26.9309271596588	20.81305270452266
27	18.3266083398769	34.06169913226676	29.333936912282145	18.277755615574197
28	16.3959300901585	28.96813886386883	37.31352496964958	17.322406076323087
29	17.048605141881986	24.989097744610437	35.93297087620945	22.029326237298125
30	19.666208451123047	28.9123166612349	31.933220449909694	19.48825443773236
31	27.826604689197776	27.350954387087484	25.428307631446025	19.394133292268727
32	28.056464722160086	27.826604689197776	26.84025756536873	17.27667302327341
33	25.059589039732	28.81487671656592	26.68234909917833	19.44318514452375
34	20.33016742014471	28.865654344407726	27.91322534845732	22.89095288699024
35	19.861685724317972	27.219795442492178	30.607625671310107	22.31089316187974
36	27.91143319688644	24.83742862092609	28.673562246402255	18.57757593578522
37	21.42530478192502	30.865297241613227	29.48348200447507	18.22591597198668
38	20.09639120412009	30.87658115891141	25.367839109924606	23.6591885270439
39	20.830907844247427	29.292053666310668	28.876407253833047	21.000631235608857
40	22.641379186748168	26.503864077914784	27.048611779480396	23.806144955856652
41	17.71946721325077	25.81176169163178	29.532732984682415	26.936038110435035
42	22.668792468184332	25.603872109408858	26.518931426307056	25.208403996099747
43	23.653347440442488	25.07366074836267	27.460408384879813	23.812583426315022
44	20.21699636724239	30.596806385900678	28.130739448375746	21.055457798481182
45	19.284546542508178	34.244963224386005	24.777955739166277	21.69253449393954
46	23.63861197197075	30.402258376483253	26.145301011769124	19.81382863977687
47	21.558587758011747	29.200122928322564	27.14352943675331	22.097759876912377
48	23.4868764723023	24.84380071540036	29.848018909190355	21.821303903106994
49	21.646602312937542	23.57947097013147	31.3062992800197	23.467627436911286
50	19.99729185984844	30.18846133167482	28.422461898525725	21.39178490995102
51	18.97118552153934	32.21253059103167	26.208291820686608	22.60799206674238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	646.0
1	1219.0
2	1792.0
3	5410.0
4	9028.0
5	6759.0
6	4490.0
7	4348.5
8	4207.0
9	4523.0
10	4839.0
11	4711.5
12	4584.0
13	4394.5
14	4205.0
15	4122.5
16	4040.0
17	3764.5
18	3489.0
19	3687.5
20	3886.0
21	3896.0
22	3906.0
23	4633.0
24	5360.0
25	6982.0
26	11476.0
27	14348.0
28	16507.0
29	18666.0
30	22956.0
31	27246.0
32	33213.0
33	39180.0
34	43460.0
35	47740.0
36	48721.5
37	49703.0
38	58342.5
39	66982.0
40	86590.0
41	106198.0
42	127983.5
43	149769.0
44	152250.5
45	154732.0
46	144298.0
47	133864.0
48	130072.5
49	126281.0
50	120271.0
51	114261.0
52	105491.5
53	96722.0
54	85663.5
55	74605.0
56	67208.5
57	59812.0
58	54903.0
59	49994.0
60	45360.0
61	40726.0
62	34203.0
63	27680.0
64	22690.0
65	17700.0
66	14330.5
67	10961.0
68	8732.5
69	6504.0
70	5357.0
71	4210.0
72	3394.0
73	2578.0
74	1991.5
75	1100.5
76	796.0
77	606.0
78	416.0
79	290.5
80	165.0
81	147.5
82	130.0
83	93.0
84	56.0
85	42.5
86	29.0
87	26.0
88	23.0
89	13.0
90	3.0
91	4.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1506569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.633275323850583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.38177453433893	15.977907857762084
2	7.190783065901953	2.823572474538709
3	2.044533976118727	1.2042269538631776
4	1.015449469804472	0.797463960725172
5	0.5584941017972742	0.548253423366626
6	0.4061651927788612	0.47846118340753385
7	0.3260222619727292	0.4480619381710594
8	0.25737289128087937	0.4042458268330375
9	0.2243966836042689	0.396507768386544
>10	3.431615141578316	17.65904694177432
>50	2.0821430497410116	29.7894909668413
>100	1.078537182964506	28.67427993276638
>500	0.0020343360885843966	0.2705981105800641
>1k	3.390560147640661E-4	0.17740318934707772
>5k	3.390560147640661E-4	0.3504794716369096
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5265	0.34946955632300947	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2665	0.1768919976449801	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.637598410693437E-5	0.0	0.0	0.03318799205346718	0.0
2	6.637598410693437E-5	0.0	0.0	0.12485322610514354	0.0
3	6.637598410693437E-5	0.0	0.0	0.18432610786495673	0.0
4	6.637598410693437E-5	0.0	0.0	0.27917738915376594	0.0
5	6.637598410693437E-5	0.0	0.0	0.490518522550245	0.0
6	6.637598410693437E-5	0.0	0.0	0.7009303921692269	0.0
7	6.637598410693437E-5	0.0	0.0	0.8311600729870321	0.0
8	6.637598410693437E-5	0.0	0.0	1.2022682001289022	0.0
9	6.637598410693437E-5	0.0	0.0	1.333891776612953	0.0
10	6.637598410693437E-5	0.0	0.0	1.5900366992816126	0.0
11	6.637598410693437E-5	0.0	0.0	1.9046588639484816	0.0
12	6.637598410693437E-5	0.0	0.0	2.14978537325539	0.0
13	6.637598410693437E-5	0.0	0.0	2.245366790369376	0.0
14	6.637598410693437E-5	0.0	0.0	2.2810106938347996	0.0
15	6.637598410693437E-5	0.0	0.0	2.3439351267681734	0.0
16	6.637598410693437E-5	0.0	0.0	2.5004496972923245	0.0
17	6.637598410693437E-5	0.0	0.0	2.693072803170648	0.0
18	6.637598410693437E-5	0.0	0.0	2.9569173399957123	0.0
19	6.637598410693437E-5	0.0	0.0	3.0801775424822893	0.0
20	6.637598410693437E-5	0.0	0.0	3.2215583886300596	0.0
21	6.637598410693437E-5	0.0	0.0	3.3967909866723662	0.0
22	6.637598410693437E-5	0.0	0.0	3.5884184527890857	0.0
23	6.637598410693437E-5	0.0	0.0	3.7988966983921744	0.0
24	6.637598410693437E-5	0.0	0.0	3.950897701997054	0.0
25	6.637598410693437E-5	0.0	0.0	4.072830384801493	0.0
26	6.637598410693437E-5	0.0	0.0	4.2027281856987635	0.0
27	6.637598410693437E-5	0.0	0.0	4.32167394921839	0.0
28	6.637598410693437E-5	0.0	0.0	4.452567389877264	0.0
29	6.637598410693437E-5	0.0	0.0	4.605099401354999	0.0
30	6.637598410693437E-5	0.0	0.0	4.78305341474569	0.0
31	6.637598410693437E-5	0.0	0.0	4.963264211596017	0.0
32	6.637598410693437E-5	0.0	0.0	5.112278295916084	0.0
33	6.637598410693437E-5	0.0	0.0	5.264544803457392	0.0
34	6.637598410693437E-5	0.0	0.0	5.425639316884922	0.0
35	6.637598410693437E-5	0.0	0.0	5.629015332188569	0.0
36	6.637598410693437E-5	0.0	0.0	5.810022640848179	0.0
37	6.637598410693437E-5	0.0	0.0	5.989636053841544	0.0
38	6.637598410693437E-5	0.0	0.0	6.169979602660084	0.0
39	6.637598410693437E-5	0.0	0.0	6.347004352273278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTT	25	3.8913844E-5	45.000004	14
TACGATA	25	3.8913844E-5	45.000004	36
CGAATTA	25	3.8913844E-5	45.000004	17
TAGTCGG	25	3.8913844E-5	45.000004	2
CATTGCG	20	7.033985E-4	45.0	13
GTTACGT	20	7.033985E-4	45.0	15
CGCTATA	20	7.033985E-4	45.0	29
CGTAACT	40	6.8175723E-9	45.0	33
CCGGTTA	20	7.033985E-4	45.0	28
ATACGCG	35	1.2122655E-7	45.0	1
TACGTAC	20	7.033985E-4	45.0	10
AGTCGAC	20	7.033985E-4	45.0	38
CGTATCG	30	2.1659525E-6	44.999996	34
CGCGAAC	30	2.1659525E-6	44.999996	42
TACGTCA	30	2.1659525E-6	44.999996	38
TATAGCG	190	0.0	42.63158	1
TCGATTG	75	0.0	42.0	1
TTTAGCG	145	0.0	41.89655	1
TCGTCCC	145	0.0	41.89655	38
CGCCGGT	140	0.0	41.785713	28
>>END_MODULE