FastQCFastQC Report
Thu 26 May 2016
SRR1548067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548067_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1478796
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120320.8136348759396157No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18530.12530463972042122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75700.043.4246981
GCGTATG800.042.18751
CTAGCGG1600.040.781252
ACGGGAC4150.040.662655
AACGGGA5950.040.4621854
ACGGGAT7650.039.4117625
GCGGGTC1600.039.3755
CACCGAA403.4591176E-739.37524
CGTAAGG2350.039.2553182
TACCGGG1950.039.230773
TAGTGCG750.039.0000041
TACGGGA6200.038.8306434
TCTAGCG356.2495037E-638.571431
GGCGATT5550.038.108118
GTTAGCG659.094947E-1238.0769271
CGACCCG950.037.89473732
CGACGGT1550.037.74193628
CGGTCTA1550.037.74193631
CGGCGAT301.1400799E-437.50000422
ACTCGCG301.1400799E-437.5000041
Produced by FastQC (version 0.11.5)