##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548067_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1478796
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46763853837852	31.0	31.0	33.0	30.0	34.0
2	31.857786334288164	31.0	31.0	34.0	30.0	34.0
3	31.80299852041796	31.0	31.0	34.0	30.0	34.0
4	35.604224653028545	37.0	35.0	37.0	33.0	37.0
5	35.56952818373866	37.0	35.0	37.0	33.0	37.0
6	35.651693675124896	37.0	35.0	37.0	33.0	37.0
7	36.03921433382292	37.0	35.0	37.0	35.0	37.0
8	36.07284845238965	37.0	35.0	37.0	35.0	37.0
9	37.87857216275943	39.0	38.0	39.0	35.0	39.0
10	37.261389671056726	39.0	37.0	39.0	34.0	39.0
11	36.98857110784719	39.0	37.0	39.0	33.0	39.0
12	36.51614691952101	39.0	35.0	39.0	32.0	39.0
13	36.33380331026051	39.0	35.0	39.0	32.0	39.0
14	37.400895728687395	40.0	35.0	41.0	32.0	41.0
15	37.56737778571216	40.0	35.0	41.0	32.0	41.0
16	37.66314894008369	40.0	35.0	41.0	33.0	41.0
17	37.60423885377023	40.0	35.0	41.0	33.0	41.0
18	37.46686223116644	39.0	36.0	41.0	32.0	41.0
19	37.37272348586282	39.0	36.0	41.0	32.0	41.0
20	37.20321802331085	39.0	35.0	41.0	32.0	41.0
21	37.09447077216871	39.0	35.0	41.0	32.0	41.0
22	37.0045841346609	39.0	35.0	41.0	32.0	41.0
23	36.91271006954306	38.0	35.0	41.0	32.0	41.0
24	36.91557456200855	39.0	35.0	41.0	32.0	41.0
25	36.893503228301945	38.0	35.0	41.0	32.0	41.0
26	36.75781514150701	38.0	35.0	41.0	31.0	41.0
27	36.69673369416742	38.0	35.0	41.0	31.0	41.0
28	36.62485968314764	38.0	35.0	41.0	31.0	41.0
29	36.56757794854733	38.0	35.0	40.0	31.0	41.0
30	36.46578703215319	38.0	35.0	40.0	31.0	41.0
31	36.35853626869426	38.0	35.0	40.0	30.0	41.0
32	36.168614873180616	38.0	35.0	41.0	30.0	41.0
33	35.99450093183914	38.0	35.0	41.0	30.0	41.0
34	35.76817356822713	38.0	35.0	41.0	28.0	41.0
35	35.56622008715198	38.0	35.0	41.0	27.0	41.0
36	35.390361483260705	38.0	35.0	40.0	26.0	41.0
37	35.292073416482054	38.0	34.0	40.0	25.0	41.0
38	35.1892512557513	38.0	34.0	40.0	25.0	41.0
39	35.12006524226465	38.0	34.0	40.0	24.0	41.0
40	35.00776104344345	38.0	34.0	40.0	24.0	41.0
41	34.94894157138645	38.0	34.0	40.0	24.0	41.0
42	34.88053997982142	38.0	34.0	40.0	24.0	41.0
43	34.772861165434584	38.0	34.0	40.0	23.0	41.0
44	34.61981503872069	38.0	34.0	40.0	23.0	41.0
45	34.60048377193338	38.0	34.0	40.0	23.0	41.0
46	34.52498383820351	37.0	34.0	40.0	23.0	41.0
47	34.4518479898512	37.0	33.0	40.0	23.0	41.0
48	34.32861665841671	37.0	33.0	40.0	23.0	41.0
49	34.234014022218076	37.0	33.0	40.0	23.0	41.0
50	34.076171425943805	37.0	33.0	40.0	22.0	41.0
51	33.699722612179094	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	4.0
13	8.0
14	13.0
15	43.0
16	109.0
17	244.0
18	595.0
19	1194.0
20	2155.0
21	3486.0
22	5500.0
23	8266.0
24	12684.0
25	18908.0
26	25860.0
27	28545.0
28	29658.0
29	31055.0
30	35196.0
31	42179.0
32	51237.0
33	65675.0
34	106590.0
35	158895.0
36	101909.0
37	133808.0
38	213669.0
39	401232.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.697813626761228	23.54198956448354	29.324463955812703	15.435732852942529
2	33.089215821519666	24.798822826136938	29.311412797978896	12.800548554364497
3	28.46214082266925	24.52677718901052	33.473853053429956	13.537228934890274
4	24.895320247011757	27.593934525113674	33.36741511337601	14.14333011449855
5	22.747829991425455	31.75860632568657	31.3555081295865	14.13805555330147
6	21.070722398491746	40.122775555249	28.71599598592368	10.09050606033557
7	86.62580910416312	3.3479939085580432	8.48034482105713	1.5458521662217102
8	87.77803023540773	2.364626358199508	8.198155797013246	1.6591876093795224
9	82.91677824392276	5.095158493801715	9.676453006364637	2.3116102559108898
10	39.41253560328808	33.27585414080103	17.0368326665747	10.274777589336189
11	32.462625000338114	22.668576328310326	28.650266838698506	16.21853183265305
12	30.083054052080204	21.49025288139811	30.944565714270254	17.482127352251425
13	23.303417104184756	29.13153673664251	31.062837605727907	16.50220855344483
14	18.18871568492206	32.66840050960376	31.623699279684285	17.5191845257899
15	17.35486165772696	26.641876229040378	40.67504916161526	15.328212951617399
16	20.551110497999726	24.23944884892845	38.46940348770216	16.740037165369664
17	20.72449479170893	23.482008336511594	30.8879656152708	24.905531256508674
18	21.753710450934406	25.331756374780568	33.73305040046092	19.18148277382411
19	24.499457666912814	26.226741213798256	29.974587434642775	19.29921368464616
20	26.074049429400674	26.126321683315346	29.10009223719837	18.69953665008561
21	23.325462065085382	26.582233113965685	31.497109810954317	18.595195009994615
22	22.143960356939026	23.71219559695861	31.718911871549558	22.42493217455281
23	19.471651262243068	27.795044076397286	31.115042237063122	21.61826242429652
24	19.644765065634477	25.58757259283904	35.709387907459856	19.058274434066632
25	19.479563104038693	26.691173089459262	33.48487553388026	20.34438827262178
26	19.179589341599517	30.446795906940512	30.156492173362654	20.217122578097317
27	19.10872087833616	29.42603307014625	32.520374683188216	18.944871368329373
28	17.905579944765876	27.308431994676752	36.11025455843808	18.67573350211929
29	18.545492414099037	26.103262383722974	35.15224547537321	20.19899972680478
30	20.130971411878313	26.764070230106114	33.090433027949764	20.014525330065812
31	23.96503642152129	26.29720394158491	29.85361064000714	19.884148996886655
32	24.274409722503982	27.25392819564024	30.008939705003257	18.46272237685252
33	23.619146927635725	26.65736179973438	29.891952642555157	19.831538630074736
34	20.22260000703275	27.45138612763356	31.02517182897438	21.300842036359306
35	19.979429211331382	27.03388432211069	31.768141109388992	21.218545357168942
36	24.122394163900903	26.001017043594928	29.97309973789488	19.90348905460929
37	21.137803997305916	28.74683188215278	30.769355610915905	19.3460085096254
38	21.0258210057371	29.556003667848707	27.961733734740964	21.456441591673226
39	20.559089962374795	28.534632227839406	29.46782382424621	21.43845398553959
40	21.956375321545366	26.76190630756372	29.189083551754262	22.09263481913665
41	19.785149540572196	26.59021257834076	29.632281937468047	23.992355943619
42	21.768857908731157	27.272253914671126	28.177449763185724	22.78143841341199
43	21.534545670937707	27.442054211669493	28.764346130230265	22.25905398716253
44	21.125564310425506	29.007043567875492	29.2960624724438	20.571329649255205
45	19.992953727221334	30.118353038552986	27.319860210603764	22.56883302362192
46	21.5228469646929	29.098401672712125	28.74081347258175	20.637937890013227
47	21.716450409657586	27.66818411734952	29.06147974433255	21.553885728660344
48	22.0622722809637	26.040508629993592	30.311483125461525	21.585735963581186
49	21.155994471177905	25.378077841703657	31.325010346254658	22.140917340863783
50	19.673978019956774	28.563371824105555	29.827170211442283	21.935479944495388
51	19.844251675011293	29.935907319197508	28.30721749314983	21.912623512641368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	637.0
1	1097.0
2	1557.0
3	11104.5
4	20652.0
5	15521.5
6	10391.0
7	9663.5
8	8936.0
9	9018.5
10	9101.0
11	9107.5
12	9114.0
13	8839.0
14	8564.0
15	8251.0
16	7938.0
17	7573.5
18	7209.0
19	7045.5
20	6882.0
21	7111.0
22	7340.0
23	8557.5
24	9775.0
25	10568.0
26	13732.5
27	16104.0
28	18571.0
29	21038.0
30	24432.5
31	27827.0
32	32654.0
33	37481.0
34	41962.5
35	46444.0
36	50862.0
37	55280.0
38	62307.5
39	69335.0
40	81907.0
41	94479.0
42	105288.5
43	116098.0
44	120511.0
45	124924.0
46	128027.5
47	131131.0
48	128001.0
49	124871.0
50	120278.5
51	115686.0
52	105113.0
53	94540.0
54	83873.0
55	73206.0
56	66083.0
57	58960.0
58	53655.5
59	48351.0
60	41793.5
61	35236.0
62	29847.5
63	24459.0
64	20962.5
65	17466.0
66	13948.5
67	10431.0
68	8701.5
69	6972.0
70	5444.5
71	3917.0
72	3208.0
73	2499.0
74	1787.5
75	924.0
76	772.0
77	588.0
78	404.0
79	282.5
80	161.0
81	119.5
82	78.0
83	67.5
84	57.0
85	40.5
86	24.0
87	20.0
88	16.0
89	12.5
90	9.0
91	6.0
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1478796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.129257085325005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93141206704315	21.142213129892127
2	6.335692163655253	3.437652430425647
3	2.498178348756034	2.0332116800518545
4	1.4928757526737304	1.6200244036293479
5	1.0471768212370367	1.4204564598566496
6	0.7688727031827328	1.2515367138319886
7	0.6503434450396157	1.2350334159964964
8	0.5845498286402329	1.268672206429084
9	0.529940958971637	1.2939214064386688
>10	7.181644648991013	46.14495284943417
>50	0.9327546031470396	16.248141871788665
>100	0.04480645111490602	1.6990077580039449
>500	0.0010012614557905343	0.17927896432886997
>1k	5.006307278952671E-4	0.20881924822396783
>5k	0.0	0.0
>10k+	2.5031536394763356E-4	0.8170774616685467
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12032	0.8136348759396157	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1853	0.12530463972042122	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.76225794497686E-5	0.0	0.0	0.033270309089286146	0.0
2	6.76225794497686E-5	0.0	0.0	0.11739279792479829	0.0
3	6.76225794497686E-5	0.0	0.0	0.2105767124065794	0.0
4	6.76225794497686E-5	0.0	0.0	0.31295729769352904	0.0
5	6.76225794497686E-5	0.0	0.0	0.577158715603775	0.0
6	6.76225794497686E-5	0.0	0.0	0.9893859599295644	0.0
7	6.76225794497686E-5	0.0	0.0	1.2543312262137576	0.0
8	6.76225794497686E-5	0.0	0.0	1.810662187347004	0.0
9	6.76225794497686E-5	0.0	0.0	2.0738492665655035	0.0
10	6.76225794497686E-5	0.0	0.0	2.464910643523515	0.0
11	6.76225794497686E-5	0.0	0.0	2.7775298283197953	0.0
12	6.76225794497686E-5	0.0	0.0	3.0405140397999455	0.0
13	6.76225794497686E-5	0.0	0.0	3.168929318175056	0.0
14	6.76225794497686E-5	0.0	0.0	3.2318183170633406	0.0
15	6.76225794497686E-5	0.0	0.0	3.3037011190184447	0.0
16	6.76225794497686E-5	0.0	0.0	3.4513888325367392	0.0
17	6.76225794497686E-5	0.0	0.0	3.6328202132004686	0.0
18	6.76225794497686E-5	0.0	0.0	3.874638557312841	0.0
19	6.76225794497686E-5	0.0	0.0	4.004676777594746	0.0
20	6.76225794497686E-5	0.0	0.0	4.14377642352292	0.0
21	6.76225794497686E-5	0.0	0.0	4.307625933529709	0.0
22	6.76225794497686E-5	0.0	0.0	4.5002826623821	0.0
23	6.76225794497686E-5	0.0	0.0	4.717824500472005	0.0
24	6.76225794497686E-5	0.0	0.0	4.8826207265910915	0.0
25	6.76225794497686E-5	0.0	0.0	5.031322778801133	0.0
26	6.76225794497686E-5	0.0	0.0	5.169069973140312	0.0
27	6.76225794497686E-5	0.0	0.0	5.314120406060065	0.0
28	6.76225794497686E-5	0.0	0.0	5.470328564589031	0.0
29	6.76225794497686E-5	0.0	0.0	5.642428029288691	0.0
30	6.76225794497686E-5	0.0	0.0	5.839547848384767	0.0
31	6.76225794497686E-5	0.0	0.0	6.018341948449955	0.0
32	6.76225794497686E-5	0.0	0.0	6.190035677672918	0.0
33	6.76225794497686E-5	0.0	0.0	6.374171961514637	0.0
34	6.76225794497686E-5	0.0	0.0	6.5602693001604	0.0
35	6.76225794497686E-5	0.0	0.0	6.76198745465906	0.0
36	6.76225794497686E-5	0.0	0.0	6.951736412595111	0.0
37	6.76225794497686E-5	0.0	0.0	7.147300912363842	0.0
38	6.76225794497686E-5	0.0	0.0	7.353955515162335	0.0
39	6.76225794497686E-5	0.0	0.0	7.617074971801384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7570	0.0	43.424698	1
GCGTATG	80	0.0	42.1875	1
CTAGCGG	160	0.0	40.78125	2
ACGGGAC	415	0.0	40.66265	5
AACGGGA	595	0.0	40.462185	4
ACGGGAT	765	0.0	39.411762	5
GCGGGTC	160	0.0	39.375	5
CACCGAA	40	3.4591176E-7	39.375	24
CGTAAGG	235	0.0	39.255318	2
TACCGGG	195	0.0	39.23077	3
TAGTGCG	75	0.0	39.000004	1
TACGGGA	620	0.0	38.830643	4
TCTAGCG	35	6.2495037E-6	38.57143	1
GGCGATT	555	0.0	38.10811	8
GTTAGCG	65	9.094947E-12	38.076927	1
CGACCCG	95	0.0	37.894737	32
CGACGGT	155	0.0	37.741936	28
CGGTCTA	155	0.0	37.741936	31
CGGCGAT	30	1.1400799E-4	37.500004	22
ACTCGCG	30	1.1400799E-4	37.500004	1
>>END_MODULE