FastQCFastQC Report
Thu 26 May 2016
SRR1548066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548066_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1899996
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191711.0090021242149982No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60280.31726382581858065No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34460.18136880288169027No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19090.10047389573451734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG351.2124838E-745.00000412
GCCACGG600.045.0000042
CTATGCG453.8562575E-1045.01
CGAACTA253.8917722E-545.021
GCGACTA253.8917722E-545.09
GTCGATC207.0344517E-445.015
ATCCGCG253.8917722E-545.01
TAACACG207.0344517E-445.01
GTCACGA253.8917722E-545.019
TATTACG207.0344517E-445.01
GCGATAC207.0344517E-445.09
TACTCGA253.8917722E-545.032
ACGTCCG253.8917722E-545.01
CCGGTTA207.0344517E-445.030
ACGAGTT253.8917722E-545.030
TCGGTCA207.0344517E-445.039
TACGTCG207.0344517E-445.010
CGTTTTT95750.043.6370771
CCAGTCG1950.042.6923128
CTCACGA6650.042.63157724