##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548066_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1899996
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50977949427262	31.0	31.0	33.0	30.0	34.0
2	31.88882608173912	31.0	31.0	34.0	30.0	34.0
3	31.832234909968232	33.0	31.0	34.0	30.0	34.0
4	35.62350815475401	37.0	35.0	37.0	33.0	37.0
5	35.57019804252219	37.0	35.0	37.0	33.0	37.0
6	35.65735296284834	37.0	35.0	37.0	33.0	37.0
7	36.050055894854516	37.0	35.0	37.0	35.0	37.0
8	36.0867638668713	37.0	35.0	37.0	35.0	37.0
9	37.92299615367611	39.0	38.0	39.0	35.0	39.0
10	37.290734822599624	39.0	37.0	39.0	34.0	39.0
11	36.97292415352453	39.0	37.0	39.0	33.0	39.0
12	36.1231081539119	37.0	35.0	39.0	32.0	39.0
13	35.77990374716578	37.0	35.0	39.0	31.0	39.0
14	36.801410108231806	38.0	35.0	41.0	31.0	41.0
15	37.06571855940749	38.0	35.0	41.0	32.0	41.0
16	37.22832679647747	38.0	35.0	41.0	33.0	41.0
17	37.17904616641298	38.0	35.0	41.0	33.0	41.0
18	37.091707561489606	38.0	35.0	41.0	32.0	41.0
19	36.977222057309596	38.0	35.0	41.0	32.0	41.0
20	36.778593744407885	38.0	35.0	41.0	32.0	41.0
21	36.64124871841835	38.0	35.0	40.0	32.0	41.0
22	36.5275126895004	38.0	35.0	40.0	32.0	41.0
23	36.45864464977821	38.0	35.0	40.0	31.0	41.0
24	36.45612727605742	37.0	35.0	40.0	32.0	41.0
25	36.4033255859486	37.0	35.0	40.0	31.0	41.0
26	36.27683321438572	37.0	35.0	40.0	31.0	41.0
27	36.200459895705045	37.0	35.0	40.0	31.0	41.0
28	36.165748769997414	37.0	35.0	40.0	31.0	41.0
29	36.108931281960594	37.0	35.0	40.0	31.0	41.0
30	36.04642957143068	37.0	35.0	40.0	31.0	41.0
31	35.898606102328635	37.0	35.0	40.0	30.0	41.0
32	35.67356510224232	37.0	35.0	40.0	30.0	41.0
33	35.51909109282335	37.0	34.0	40.0	30.0	41.0
34	35.345539674820365	37.0	34.0	40.0	29.0	41.0
35	35.1614403398744	37.0	34.0	40.0	27.0	41.0
36	34.940097768626885	36.0	34.0	40.0	26.0	41.0
37	34.81119381303961	36.0	34.0	40.0	25.0	41.0
38	34.705636222392044	36.0	34.0	40.0	25.0	41.0
39	34.69252251057371	36.0	34.0	40.0	25.0	41.0
40	34.51037265341611	36.0	34.0	40.0	24.0	41.0
41	34.44646251886846	36.0	34.0	40.0	23.0	41.0
42	34.36928709323598	36.0	34.0	40.0	23.0	41.0
43	34.214727820479624	35.0	33.0	40.0	23.0	41.0
44	34.03224743631039	35.0	33.0	40.0	23.0	41.0
45	33.98166311929078	35.0	33.0	40.0	23.0	41.0
46	33.84714599399157	35.0	33.0	40.0	23.0	41.0
47	33.76021738993135	35.0	33.0	40.0	23.0	41.0
48	33.6441550403264	35.0	33.0	40.0	22.0	41.0
49	33.54677167741406	35.0	33.0	40.0	22.0	41.0
50	33.365757612121286	35.0	33.0	40.0	20.0	41.0
51	32.97698205680433	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	8.0
13	17.0
14	16.0
15	56.0
16	138.0
17	293.0
18	707.0
19	1412.0
20	2698.0
21	4496.0
22	7189.0
23	10945.0
24	16916.0
25	25144.0
26	33367.0
27	36733.0
28	38146.0
29	42205.0
30	48851.0
31	58921.0
32	73343.0
33	96257.0
34	174070.0
35	306147.0
36	131501.0
37	148395.0
38	221558.0
39	420359.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.9774315314348	25.81915961928341	29.333956492539986	14.869452356741803
2	31.698382522910574	25.82263331080697	29.621062360131283	12.85792180615117
3	27.23784681651961	24.531367434457756	34.37212499394736	13.858660755075276
4	24.335682811963817	27.288899555578013	33.898755576327524	14.476662056130646
5	22.336415445085148	31.148539260082654	32.064593820197516	14.450451474634685
6	19.89419977726269	40.062294867989195	28.966113612870764	11.077391741877351
7	86.25728685744602	3.839797557468542	8.07449068313828	1.8284249019471621
8	87.38586818077512	2.8680060379074486	7.804753273164786	1.9413725081526487
9	83.53786007970542	4.72085204389904	9.283809018545302	2.457478857850227
10	47.5300474316788	23.601154949799895	17.194825673317208	11.673971945204094
11	41.06992856827067	21.90573032785332	23.24268051090634	13.781660592969668
12	38.79860799706947	20.96772835311232	25.680159326651214	14.553504323166996
13	20.431885119758146	40.042873774471104	25.277000583159122	14.248240522611628
14	14.455925170368781	41.64371924993526	27.95547990627349	15.944875673422471
15	13.06876435529338	26.417739826820686	45.48204312009078	15.031452697795153
16	13.923660891917667	21.49678209848863	46.34504493693671	18.234512072656994
17	14.947610416021927	23.587944395672412	28.858481807330122	32.60596338097554
18	19.540620085515968	26.453897797679577	34.31028275849002	19.69519935831444
19	24.899210314126975	29.36016707403595	25.504685273021625	20.23593733881545
20	27.300373263943712	25.18668460354653	27.480636801340637	20.032305331169116
21	19.485146284518493	31.22059204335167	29.451535687443553	19.842725984686282
22	21.413676660371916	26.2280025852686	27.072951732529965	25.285369021829517
23	17.102983374701843	32.66812140657138	26.192370931307224	24.036524287419553
24	18.530144273987943	25.791896403992432	38.240501558950655	17.437457763068977
25	15.757296331150169	29.135113968660985	34.508598965471506	20.598990734717336
26	15.359242861563919	35.91760193179354	27.997164204556217	20.725991002086317
27	16.840035452706218	35.8525491632614	29.306272223730996	18.00114316030139
28	14.225819422777732	29.925799843789147	38.53866008138965	17.309720652043477
29	14.39171450887265	25.889159766652142	38.19139619241304	21.527729532062175
30	16.677508794755358	32.60938444080935	32.55175274053209	18.161354023903208
31	25.387948185154073	29.3745881570277	26.40768717407826	18.829776483739966
32	25.301526950583053	29.663746660519287	27.323215417295614	17.711510971602046
33	23.92036614813926	30.486379971326254	26.418950355684963	19.174303524849527
34	16.962614658136122	29.572588573870682	30.129642378194482	23.335154389798713
35	18.400407158751914	27.61868972355731	32.029962168341406	21.950940949349366
36	26.09968652565584	26.610845496516834	28.255901591371774	19.03356638645555
37	19.001776845845992	31.080486485234704	31.247486836814392	18.67024983210491
38	18.28824902789269	32.5100158105596	27.52774216366771	21.673992997879996
39	18.364143924513527	32.215594138092925	28.25311211181497	21.16714982557858
40	22.481678908797704	28.938376712372026	26.5604769694252	22.01946740940507
41	16.499403156638223	25.43926408266123	30.179379325009105	27.881953435691443
42	20.198726734161546	27.987901027160056	26.17863406028223	25.634738178396166
43	20.786727972058888	29.34511441076718	26.462792553247482	23.40536506392645
44	18.818355407064015	32.18254143692934	27.346636519234778	21.652466636771866
45	16.934140913980873	37.16049928526166	23.216259402651374	22.689100398106103
46	20.99430735643654	34.7042309562757	25.881265013189502	18.42019667409826
47	20.165200347790204	29.339798610102335	28.37711237286815	22.117888669239303
48	20.924675630896065	26.30868696565677	31.312434341967037	21.45420306148013
49	18.823565944349358	26.066054875905003	31.443803039585344	23.666576140160295
50	17.807458542017983	32.094278093217035	29.042903248217367	21.055360116547615
51	16.905930328274376	33.318438565133825	25.615738138396082	24.159892968195724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1508.0
1	1960.5
2	2413.0
3	14375.5
4	26338.0
5	19133.5
6	11929.0
7	11706.5
8	11484.0
9	13155.0
10	14826.0
11	15678.5
12	16531.0
13	16457.0
14	16383.0
15	15094.5
16	13806.0
17	12603.5
18	11401.0
19	10667.5
20	9934.0
21	9301.5
22	8669.0
23	9335.0
24	10001.0
25	10694.5
26	13716.5
27	16045.0
28	19695.0
29	23345.0
30	27576.5
31	31808.0
32	37957.5
33	44107.0
34	50705.0
35	57303.0
36	60984.0
37	64665.0
38	74337.5
39	84010.0
40	109814.5
41	135619.0
42	159780.0
43	183941.0
44	188879.0
45	193817.0
46	192712.0
47	191607.0
48	182422.0
49	173237.0
50	161103.5
51	148970.0
52	131630.5
53	114291.0
54	98057.0
55	81823.0
56	70366.0
57	58909.0
58	50418.0
59	41927.0
60	35970.5
61	30014.0
62	25114.0
63	20214.0
64	17589.0
65	14964.0
66	12055.0
67	9146.0
68	7017.0
69	4888.0
70	4140.0
71	3392.0
72	2787.5
73	2183.0
74	1604.5
75	811.5
76	597.0
77	549.5
78	502.0
79	464.5
80	427.0
81	308.5
82	190.0
83	163.5
84	137.0
85	122.5
86	108.0
87	88.5
88	69.0
89	58.0
90	47.0
91	37.0
92	27.0
93	21.0
94	15.0
95	14.5
96	14.0
97	7.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1899996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.44937528894133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11831743717231	20.13511752688189
2	8.540296507686868	4.346904218059162
3	3.127487448794682	2.3877780538748867
4	1.615383862230752	1.6444204058243963
5	1.0124386393290605	1.288296544465519
6	0.6848381839738771	1.0457222373688946
7	0.5115614716306406	0.9113243912423898
8	0.3820995181644809	0.7779355228393222
9	0.32852827587278144	0.7524755447143738
>10	2.936829833494422	16.163054682627383
>50	0.8984180862526487	16.862872470450398
>100	0.8398470453557827	31.499502306486892
>500	0.0024970673947429457	0.3897660055369657
>1k	0.001040444747809561	0.4603581367028319
>5k	2.0808894956191216E-4	0.3192268317088083
>10k+	2.0808894956191216E-4	1.0152451212159197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19171	1.0090021242149982	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6028	0.31726382581858065	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3446	0.18136880288169027	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1909	0.10047389573451734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06100012842132299	0.0
2	0.0	0.0	0.0	0.21215834138598186	0.0
3	0.0	0.0	0.0	0.3686849867052352	0.0
4	0.0	0.0	0.0	0.5559485388390292	0.0
5	0.0	0.0	0.0	1.0530022168467723	0.0
6	0.0	0.0	0.0	1.7157930858801809	0.0
7	0.0	0.0	0.0	2.164530872696574	0.0
8	0.0	0.0	0.0	3.04605904433483	0.0
9	0.0	0.0	0.0	3.468691513034764	0.0
10	0.0	0.0	0.0	4.100798106943383	0.0
11	0.0	0.0	0.0	4.727062583289649	0.0
12	0.0	0.0	0.0	5.1755372116572875	0.0
13	0.0	0.0	0.0	5.385590285453232	0.0
14	0.0	0.0	0.0	5.460590443348302	0.0
15	0.0	0.0	0.0	5.572116993930513	0.0
16	0.0	0.0	0.0	5.8539070608569705	0.0
17	0.0	0.0	0.0	6.17622352889164	0.0
18	0.0	0.0	0.0	6.593961250444738	0.0
19	0.0	0.0	0.0	6.7932774595314935	0.0
20	0.0	0.0	0.0	7.00227789953242	0.0
21	0.0	0.0	0.0	7.304594325461738	0.0
22	0.0	0.0	0.0	7.602700216210981	0.0
23	0.0	0.0	0.0	7.963543081143329	0.0
24	0.0	0.0	0.0	8.216333087017025	0.0
25	0.0	0.0	0.0	8.442017772668995	0.0
26	0.0	0.0	0.0	8.645386621866573	0.0
27	0.0	0.0	0.0	8.848808102753901	0.0
28	0.0	0.0	0.0	9.07680858275491	0.0
29	0.0	0.0	0.0	9.308388017658984	0.0
30	0.0	0.0	0.0	9.595546516940036	0.0
31	0.0	0.0	0.0	9.864231293118511	0.0
32	0.0	0.0	0.0	10.102968637828711	0.0
33	0.0	0.0	0.0	10.345705990959981	0.0
34	0.0	0.0	0.0	10.589285451127266	0.0
35	0.0	0.0	0.0	10.903180848801787	0.0
36	0.0	0.0	0.0	11.15912875606054	0.0
37	5.263168975092579E-5	0.0	0.0	11.4354451272529	0.0
38	5.263168975092579E-5	0.0	0.0	11.704235166810877	0.0
39	5.263168975092579E-5	5.263168975092579E-5	0.0	11.968656776119529	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTCG	35	1.2124838E-7	45.000004	12
GCCACGG	60	0.0	45.000004	2
CTATGCG	45	3.8562575E-10	45.0	1
CGAACTA	25	3.8917722E-5	45.0	21
GCGACTA	25	3.8917722E-5	45.0	9
GTCGATC	20	7.0344517E-4	45.0	15
ATCCGCG	25	3.8917722E-5	45.0	1
TAACACG	20	7.0344517E-4	45.0	1
GTCACGA	25	3.8917722E-5	45.0	19
TATTACG	20	7.0344517E-4	45.0	1
GCGATAC	20	7.0344517E-4	45.0	9
TACTCGA	25	3.8917722E-5	45.0	32
ACGTCCG	25	3.8917722E-5	45.0	1
CCGGTTA	20	7.0344517E-4	45.0	30
ACGAGTT	25	3.8917722E-5	45.0	30
TCGGTCA	20	7.0344517E-4	45.0	39
TACGTCG	20	7.0344517E-4	45.0	10
CGTTTTT	9575	0.0	43.637077	1
CCAGTCG	195	0.0	42.69231	28
CTCACGA	665	0.0	42.631577	24
>>END_MODULE