FastQCFastQC Report
Thu 26 May 2016
SRR1548065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548065_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4747748
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676391.4246543835098242No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.13065141620827392No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59800.12595445251095888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT5550.043.78378328
CGTTTTT344300.043.7322121
TATAGCG2800.042.5892831
GGGCGAT57100.039.6409847
CACGACG6150.039.1463426
ACGGGAT21200.038.6320765
TAGGGAC49300.038.2454385
TAGGGAT74450.037.9885835
CGGTCTA6350.037.91338731
TAACGGG13650.037.9120863
TAGCGGG30500.037.7704933
CATACGA8700.037.7586218
CGTTAGG7250.037.5517232
CGGTTTT30350.037.438221
AGGGCGA30450.037.389166
TCACGAC6550.037.09923625
AGGGATC56000.037.0848246
AGGGATT80800.036.9801986
TAAGGGA62000.036.6895144
TCGTCCC10800.036.66666838