##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548065_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4747748
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450959486476535	31.0	31.0	33.0	30.0	34.0
2	31.822698466725697	31.0	31.0	34.0	30.0	34.0
3	31.675391364495336	31.0	31.0	34.0	30.0	34.0
4	35.528522996587014	37.0	35.0	37.0	33.0	37.0
5	35.51769428368987	37.0	35.0	37.0	33.0	37.0
6	35.61532351759192	37.0	35.0	37.0	33.0	37.0
7	36.02763562851272	37.0	35.0	37.0	35.0	37.0
8	36.067192698517275	37.0	35.0	37.0	35.0	37.0
9	37.881765628672795	39.0	38.0	39.0	35.0	39.0
10	37.3010001794535	39.0	37.0	39.0	34.0	39.0
11	36.94370699540077	39.0	37.0	39.0	33.0	39.0
12	36.515285562755224	39.0	35.0	39.0	32.0	39.0
13	36.35636748201463	39.0	35.0	39.0	32.0	39.0
14	37.45997070611161	40.0	35.0	41.0	32.0	41.0
15	37.61307529380245	40.0	35.0	41.0	32.0	41.0
16	37.691481940490526	40.0	35.0	41.0	33.0	41.0
17	37.62449691938157	40.0	35.0	41.0	33.0	41.0
18	37.453905725409186	39.0	36.0	41.0	32.0	41.0
19	37.317778449909305	39.0	36.0	41.0	32.0	41.0
20	37.10226701164426	39.0	35.0	41.0	32.0	41.0
21	36.991125476752345	39.0	35.0	41.0	32.0	41.0
22	36.92828431500577	38.0	35.0	41.0	32.0	41.0
23	36.836449196545395	38.0	35.0	41.0	32.0	41.0
24	36.79605130685116	38.0	35.0	41.0	32.0	41.0
25	36.763016697600634	38.0	35.0	41.0	31.0	41.0
26	36.64208588998405	38.0	35.0	41.0	31.0	41.0
27	36.583334667299106	38.0	35.0	41.0	31.0	41.0
28	36.51044642638994	38.0	35.0	40.0	31.0	41.0
29	36.425842736387864	38.0	35.0	40.0	31.0	41.0
30	36.30876533463866	38.0	35.0	40.0	30.0	41.0
31	36.17140821290431	38.0	35.0	40.0	30.0	41.0
32	35.93175290685184	38.0	35.0	40.0	30.0	41.0
33	35.69210560459401	38.0	35.0	40.0	28.0	41.0
34	35.416664068943845	38.0	34.0	40.0	26.0	41.0
35	35.18067618584643	38.0	34.0	40.0	25.0	41.0
36	34.98240176184583	38.0	34.0	40.0	24.0	41.0
37	34.87297282838095	38.0	34.0	40.0	23.0	41.0
38	34.74569838163272	38.0	34.0	40.0	23.0	41.0
39	34.67784389567433	38.0	34.0	40.0	23.0	41.0
40	34.55637704444297	38.0	34.0	40.0	23.0	41.0
41	34.48872202147207	38.0	34.0	40.0	22.0	41.0
42	34.44028790070577	38.0	34.0	40.0	22.0	41.0
43	34.3300609046647	38.0	33.0	40.0	22.0	41.0
44	34.162439961008886	37.0	33.0	40.0	21.0	41.0
45	34.14558923514896	37.0	33.0	40.0	21.0	41.0
46	34.07103936434706	37.0	33.0	40.0	21.0	41.0
47	33.98416786232125	37.0	33.0	40.0	20.0	41.0
48	33.85953277216904	37.0	33.0	40.0	20.0	41.0
49	33.759499872360536	37.0	33.0	40.0	20.0	41.0
50	33.5956396590552	36.0	33.0	40.0	20.0	41.0
51	33.243498391237274	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	6.0
12	12.0
13	30.0
14	64.0
15	144.0
16	329.0
17	839.0
18	2097.0
19	4251.0
20	7719.0
21	12623.0
22	19637.0
23	29893.0
24	46206.0
25	70868.0
26	96013.0
27	106844.0
28	106355.0
29	108691.0
30	119254.0
31	138640.0
32	166630.0
33	212879.0
34	340459.0
35	497236.0
36	334137.0
37	421153.0
38	659012.0
39	1245509.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.777370660784857	22.873623452634806	28.779602455732693	17.569403430847636
2	34.47781980003993	24.57883190093493	28.66997153176622	12.27337676725892
3	27.700311810989124	24.043546540380827	35.282432850269224	12.973708798360823
4	23.969658878272394	26.875373334894775	35.00042546487303	14.1545423219598
5	21.894527679228133	30.97620176976537	33.66214887563535	13.467121675371144
6	20.21038184840476	39.09617780893173	30.50654752526882	10.186892817394689
7	84.86522452328978	2.907483716490429	10.627922964740335	1.5993687954794569
8	85.76777874478594	2.1074833794885492	10.378689012137965	1.7460488635875366
9	81.23731503862463	4.658924610152013	11.687477936908193	2.416282414315166
10	41.60707350095245	31.31703704577412	17.277222801210172	9.798666652063252
11	29.239420457867602	22.85428796979115	31.432670815721476	16.47362075661977
12	29.191250251698282	20.845693579355938	32.28501175715308	17.67804441179271
13	22.3951439714155	27.646939138303043	32.601245895948985	17.35667099433247
14	17.2498413984904	30.96274275719773	33.40143579650815	18.385980047803717
15	16.26280501829499	26.44525362340208	40.80597790784178	16.485963450461146
16	20.20772795860269	24.24437859802163	38.89812601679786	16.64976742657782
17	20.07101050856111	24.00493876254595	32.0008138595393	23.92323686935364
18	20.88617593014625	24.951282165776277	35.24336169484985	18.91918020922762
19	23.422009761259442	27.067738220309923	30.643875791217223	18.866376227213408
20	24.58614062919936	26.598863292660013	30.489444679877703	18.325551398262924
21	21.86442919885386	26.82947789141294	33.11144567908828	18.19464723064493
22	21.864366011001426	23.199504270235067	33.04617262752783	21.889957091235676
23	19.08671226863768	27.438714101927903	32.20151954147524	21.273054087959174
24	19.529932928200907	25.909715511438264	36.01747607497281	18.54287548538802
25	19.284785123389025	27.002296667809667	33.676155516257396	20.036762692543917
26	18.65625555526536	30.321070115768574	31.106474058859064	19.916200270107005
27	18.33976866505973	28.974642293567392	33.23400905018548	19.4515799911874
28	17.004251278711507	27.309853008205152	36.33095101087926	19.354944702204076
29	17.875780264664424	25.52037302738056	35.98274381875365	20.621102889201364
30	19.023208476945282	27.455037630472383	33.75155968682415	19.77019420575818
31	22.702868812750804	26.488790053726525	30.54252247591911	20.265818657603564
32	22.65554111128055	27.997505343586056	30.255270498771207	19.09168304636219
33	21.849917055412376	27.594703846960705	30.17868682162575	20.37669227600117
34	18.617542464343096	28.768839458202077	31.521597186708313	21.092020890746518
35	18.371657467919526	28.428994125214736	31.676512738249794	21.52283566861594
36	22.233972822483416	27.755832870657837	29.6991542095326	20.311040097326146
37	19.40656917763959	29.90146065039678	30.87594371057605	19.81602646138759
38	19.21856425404213	29.93176975694582	29.059061264414204	21.790604724597852
39	19.340769560642222	29.222759927443498	30.36079421232972	21.07567629958456
40	21.202052004445054	27.74751313675452	29.4678866696379	21.582548189162527
41	18.074169058677924	27.121447894875633	30.617505394136334	24.186877652310105
42	20.06804067949689	27.91588770086365	29.263347591321192	22.75272402831827
43	20.131502345954335	28.29846908471132	29.4317432180478	22.138285351286548
44	19.88125738771308	29.708611324779667	29.66553827203971	20.744593015467544
45	19.055834471416762	30.939847692000498	27.992829442506217	22.011488394076515
46	20.73859016948667	29.994641670113914	28.666243448472834	20.600524711926578
47	19.96133745935968	28.300764910016284	29.744480962342568	21.99341666828147
48	20.31786438538861	27.243695326710686	30.850394755576747	21.588045532323957
49	20.130470277697974	26.166384568009924	31.087938955479522	22.615206198812572
50	18.59180394578651	28.736592590845177	30.902524733831704	21.769078729536613
51	18.418332228248	29.561088751972513	29.31731001729662	22.703269002482863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2318.0
1	4195.5
2	6073.0
3	56062.5
4	106052.0
5	75944.5
6	45837.0
7	42341.0
8	38845.0
9	39267.0
10	39689.0
11	40234.5
12	40780.0
13	39991.5
14	39203.0
15	36983.0
16	34763.0
17	32804.5
18	30846.0
19	29471.0
20	28096.0
21	27443.5
22	26791.0
23	29451.0
24	32111.0
25	36111.5
26	46603.5
27	53095.0
28	60777.5
29	68460.0
30	78055.0
31	87650.0
32	99975.0
33	112300.0
34	129668.5
35	147037.0
36	161406.5
37	175776.0
38	192981.0
39	210186.0
40	248879.0
41	287572.0
42	324633.0
43	361694.0
44	389581.5
45	417469.0
46	412267.5
47	407066.0
48	395045.5
49	383025.0
50	357238.0
51	331451.0
52	304398.0
53	277345.0
54	252428.5
55	227512.0
56	209244.5
57	190977.0
58	174496.5
59	158016.0
60	138942.0
61	119868.0
62	102804.0
63	85740.0
64	71503.0
65	57266.0
66	44302.5
67	31339.0
68	25130.5
69	18922.0
70	15427.5
71	11933.0
72	9605.0
73	7277.0
74	5239.5
75	2530.0
76	1858.0
77	1456.5
78	1055.0
79	735.0
80	415.0
81	392.5
82	370.0
83	265.0
84	160.0
85	124.0
86	88.0
87	62.0
88	36.0
89	27.0
90	18.0
91	19.5
92	21.0
93	26.0
94	31.0
95	16.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4747748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.894710353279347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12763945254153	18.34499096557937
2	7.921887748779163	3.6273865091898227
3	2.640993189647231	1.813943223658701
4	1.4044969521642394	1.286222036474556
5	0.9317356400623602	1.066590880252754
6	0.6331174519249453	0.8697024408856731
7	0.4903070972989436	0.7857807283771528
8	0.3676138325952609	0.6733129775301937
9	0.2988618058519067	0.615811903257367
>10	3.065893476349339	16.1811256053305
>50	1.142598466730501	19.123102570385093
>100	0.9702677569065143	32.85895840466546
>500	0.002808446417083845	0.44683217675656195
>1k	0.0014978380890885597	0.5954261656913173
>5k	1.8722976113606996E-4	0.2611164816463525
>10k+	9.361488056803498E-5	1.4496969303191034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67639	1.4246543835098242	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6203	0.13065141620827392	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5980	0.12595445251095888	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1062617476748978E-5	0.0	0.0	0.04090360313984651	0.0
2	2.1062617476748978E-5	0.0	0.0	0.13218898728407658	0.0
3	2.1062617476748978E-5	0.0	0.0	0.2396715242679266	0.0
4	2.1062617476748978E-5	0.0	0.0	0.3541468502540573	0.0
5	2.1062617476748978E-5	0.0	0.0	0.6743828863705488	0.0
6	2.1062617476748978E-5	0.0	0.0	1.2047817196700414	0.0
7	2.1062617476748978E-5	0.0	0.0	1.579633122903743	0.0
8	2.1062617476748978E-5	0.0	0.0	2.25357369430728	0.0
9	2.1062617476748978E-5	0.0	0.0	2.611933068056687	0.0
10	2.1062617476748978E-5	0.0	0.0	3.0904967997459005	0.0
11	2.1062617476748978E-5	0.0	0.0	3.4887908962312237	0.0
12	2.1062617476748978E-5	0.0	0.0	3.808521429528273	0.0
13	2.1062617476748978E-5	0.0	0.0	3.978833754445265	0.0
14	2.1062617476748978E-5	0.0	0.0	4.069297696507903	0.0
15	2.1062617476748978E-5	0.0	0.0	4.160435642329795	0.0
16	2.1062617476748978E-5	0.0	0.0	4.328810206438926	0.0
17	2.1062617476748978E-5	0.0	0.0	4.527072624747564	0.0
18	2.1062617476748978E-5	0.0	0.0	4.791345286228334	0.0
19	2.1062617476748978E-5	0.0	0.0	4.946260837769823	0.0
20	2.1062617476748978E-5	0.0	0.0	5.1017872052181374	0.0
21	2.1062617476748978E-5	0.0	0.0	5.299860059969484	0.0
22	2.1062617476748978E-5	0.0	0.0	5.524935190326024	0.0
23	2.1062617476748978E-5	0.0	0.0	5.775643526151767	0.0
24	2.1062617476748978E-5	0.0	0.0	5.960826059007344	0.0
25	2.1062617476748978E-5	0.0	0.0	6.133328896141919	0.0
26	2.1062617476748978E-5	0.0	0.0	6.293678602992408	0.0
27	2.1062617476748978E-5	0.0	0.0	6.467066070061006	0.0
28	2.1062617476748978E-5	0.0	0.0	6.650100215933954	0.0
29	2.1062617476748978E-5	0.0	0.0	6.845666619205568	0.0
30	2.1062617476748978E-5	0.0	0.0	7.0959326400643	0.0
31	2.1062617476748978E-5	0.0	0.0	7.3060954372473015	0.0
32	2.1062617476748978E-5	0.0	0.0	7.514404724092349	0.0
33	2.1062617476748978E-5	0.0	0.0	7.717090292071104	0.0
34	2.1062617476748978E-5	0.0	0.0	7.93020185570085	0.0
35	2.1062617476748978E-5	0.0	0.0	8.174991596015627	0.0
36	2.1062617476748978E-5	0.0	0.0	8.399750787110015	0.0
37	2.1062617476748978E-5	0.0	0.0	8.634030281303893	0.0
38	4.2125234953497956E-5	0.0	0.0	8.888445637805544	0.0
39	4.2125234953497956E-5	0.0	0.0	9.224246948237354	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	555	0.0	43.783783	28
CGTTTTT	34430	0.0	43.732212	1
TATAGCG	280	0.0	42.589283	1
GGGCGAT	5710	0.0	39.640984	7
CACGACG	615	0.0	39.14634	26
ACGGGAT	2120	0.0	38.632076	5
TAGGGAC	4930	0.0	38.245438	5
TAGGGAT	7445	0.0	37.988583	5
CGGTCTA	635	0.0	37.913387	31
TAACGGG	1365	0.0	37.912086	3
TAGCGGG	3050	0.0	37.770493	3
CATACGA	870	0.0	37.75862	18
CGTTAGG	725	0.0	37.551723	2
CGGTTTT	3035	0.0	37.43822	1
AGGGCGA	3045	0.0	37.38916	6
TCACGAC	655	0.0	37.099236	25
AGGGATC	5600	0.0	37.084824	6
AGGGATT	8080	0.0	36.980198	6
TAAGGGA	6200	0.0	36.689514	4
TCGTCCC	1080	0.0	36.666668	38
>>END_MODULE