FastQCFastQC Report
Thu 26 May 2016
SRR1548064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548064_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2663332
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163050.612203059926438No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31110.11680856911567916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96550.041.9937821
TCACGAC3950.039.303825
CGCGTAA750.039.031
TACGGGA11800.038.8983044
CTCACGA4000.038.81250424
GGGCGAT31450.038.7042927
ACTATCG356.25139E-638.57142623
TAAGGGA36750.038.5714264
TAGGGAC27350.038.336385
CGACGGT4000.038.25000428
AGGGATT57700.037.5136
AGGGCGA16800.037.56
CACGACG4150.037.4096426
CGGTCTA4150.037.4096431
GACCGAT7700.037.4025969
AAGGGAT44250.037.220345
GTAGGGA40500.037.111114
TAGGGAT49400.037.07495
TATTGCG1100.036.818181
ATAGGGA38250.036.7058834
Produced by FastQC (version 0.11.5)