FastQCFastQC Report
Thu 26 May 2016
SRR1548063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548063_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences940485
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55200.5869312110240993No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.3068629483723823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG900.045.0000041
TTAGGCG600.045.0000041
GCCCATA302.1650794E-645.00000424
ATTAGCG453.8380676E-1045.0000041
TAACGAG302.1650794E-645.0000041
ATGTACG302.1650794E-645.0000041
GCTTAAG650.045.0000041
AACGCCG302.1650794E-645.00000413
CGGGACG302.1650794E-645.0000046
GAACGCT302.1650794E-645.00000430
ACTCGAC302.1650794E-645.00000410
CGGTCTA1150.045.00000431
ACGTAAG453.8380676E-1045.0000041
CGTCCTT302.1650794E-645.00000412
CGATCAT302.1650794E-645.00000410
TCGACTG302.1650794E-645.00000412
TATGTCG302.1650794E-645.0000041
AAGTGCG650.045.0000041
TACCGAT302.1650794E-645.0000043
ACGGTCT1150.045.00000430
Produced by FastQC (version 0.11.5)