##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548063_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	940485
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58445057603258	31.0	31.0	33.0	30.0	34.0
2	31.979855074775248	31.0	31.0	34.0	30.0	34.0
3	32.04481730171135	33.0	31.0	34.0	30.0	34.0
4	35.74181512730134	37.0	35.0	37.0	35.0	37.0
5	35.640163319989156	37.0	35.0	37.0	33.0	37.0
6	35.70382621732404	37.0	35.0	37.0	33.0	37.0
7	36.073671563076495	37.0	35.0	37.0	35.0	37.0
8	36.112897069065426	37.0	35.0	37.0	35.0	37.0
9	37.97971472165957	39.0	39.0	39.0	35.0	39.0
10	37.43855882868945	39.0	37.0	39.0	35.0	39.0
11	36.980295273183515	39.0	35.0	39.0	33.0	39.0
12	35.35294342812485	35.0	35.0	39.0	32.0	39.0
13	34.585103430676725	35.0	33.0	39.0	27.0	39.0
14	35.31633359383722	35.0	33.0	40.0	27.0	41.0
15	35.861373652955656	35.0	35.0	40.0	31.0	41.0
16	36.20011270780502	35.0	35.0	40.0	32.0	41.0
17	36.20634353551625	35.0	35.0	40.0	32.0	41.0
18	36.18823373046885	36.0	35.0	40.0	32.0	41.0
19	36.09400787891354	36.0	35.0	40.0	31.0	41.0
20	35.923094998856975	35.0	35.0	40.0	31.0	41.0
21	35.71680143755616	35.0	34.0	40.0	31.0	41.0
22	35.57528190242269	35.0	34.0	40.0	31.0	41.0
23	35.61733148322408	35.0	34.0	40.0	31.0	41.0
24	35.60206489205038	35.0	34.0	40.0	31.0	41.0
25	35.5598132878249	35.0	35.0	40.0	31.0	41.0
26	35.41686789262987	35.0	34.0	40.0	31.0	41.0
27	35.35807056997188	35.0	34.0	40.0	31.0	41.0
28	35.38502368458827	35.0	34.0	40.0	31.0	41.0
29	35.476870976145285	35.0	35.0	40.0	31.0	41.0
30	35.49175265953205	36.0	35.0	40.0	31.0	41.0
31	35.28956867998958	35.0	34.0	40.0	30.0	41.0
32	35.08259887185867	35.0	34.0	40.0	30.0	41.0
33	34.9864048868403	35.0	34.0	40.0	30.0	41.0
34	34.9225208270201	35.0	34.0	40.0	30.0	41.0
35	34.83893097710224	35.0	34.0	40.0	29.0	41.0
36	34.609267558759576	35.0	34.0	40.0	28.0	41.0
37	34.45064727241795	35.0	34.0	40.0	27.0	41.0
38	34.44563815478184	35.0	34.0	40.0	27.0	41.0
39	34.520990765402956	35.0	34.0	40.0	27.0	41.0
40	34.33709415886484	35.0	34.0	39.0	27.0	41.0
41	34.40814898695886	35.0	34.0	39.0	27.0	41.0
42	34.374727932928224	35.0	34.0	39.0	27.0	41.0
43	34.21934002137195	35.0	33.0	39.0	27.0	41.0
44	34.069170693844136	35.0	34.0	39.0	27.0	41.0
45	34.06596171124473	35.0	34.0	39.0	27.0	41.0
46	34.02895846292073	35.0	34.0	39.0	27.0	41.0
47	33.99098975528584	35.0	33.0	39.0	27.0	41.0
48	33.950520210317016	35.0	34.0	39.0	27.0	41.0
49	33.92693025407104	35.0	34.0	38.0	26.0	41.0
50	33.73941317511709	35.0	33.0	38.0	24.0	41.0
51	33.31418895569839	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	7.0
14	14.0
15	25.0
16	72.0
17	171.0
18	421.0
19	834.0
20	1371.0
21	2341.0
22	3565.0
23	5210.0
24	7632.0
25	10518.0
26	13884.0
27	16365.0
28	18059.0
29	20954.0
30	25570.0
31	31175.0
32	40011.0
33	55260.0
34	119187.0
35	222149.0
36	45060.0
37	52448.0
38	84105.0
39	164028.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14043817817402	24.65855383126791	30.347533453484104	12.853474537073955
2	28.037980403727865	26.39818816887032	32.33246675917213	13.23136466822969
3	28.10454180555777	25.831140315900836	32.043785918967345	14.02053195957405
4	25.090033333864977	28.252337889493184	31.585724386885488	15.07190438975635
5	23.355290089687767	33.15438311084174	30.078416986980123	13.411909812490364
6	21.513899743217596	40.884862597489594	26.515893395428954	11.085344263863856
7	89.84609004928308	4.091186993944613	4.694599063249281	1.3681238935230227
8	91.76818343726907	2.75942731675678	4.117024726603827	1.355364519370325
9	89.03555080623295	4.042807700282301	5.254948244788594	1.6666932486961514
10	62.37643343593997	22.524654832347142	8.013843920955676	7.085067810757216
11	60.61478917792415	15.204495552826467	15.332514606825201	8.848200662424174
12	54.02276485005078	18.72512586590961	16.626208817790822	10.625900466248797
13	21.618739267505596	50.90299154159822	17.543182506898034	9.935086683998149
14	11.813798199864964	54.51867919211896	23.302657671307887	10.364864936708187
15	8.837780506866139	23.616219291110436	57.76657788268819	9.779422319335236
16	11.016656299675168	15.557930216856198	56.941365359362464	16.48404812410618
17	12.050909902869265	15.163771883655775	28.84235261593752	43.942965597537444
18	21.259775541343032	20.979494622455437	36.12231986687719	21.63840996932434
19	29.30041414801937	23.360181183112967	24.693216797716072	22.64618787115159
20	34.12951828046168	21.822144957123186	23.993365125440597	20.05497163697454
21	21.65095668724116	27.716656831315756	28.526026465068554	22.10636001637453
22	22.936250976889582	23.625682493607023	25.437300967054234	28.00076556244916
23	16.522857887153968	30.331052595203538	24.048336762415136	29.097752755227358
24	19.15458513426583	22.358782968362068	42.26670281822676	16.219929079145334
25	12.492490576670548	23.988474032015397	40.72675268611408	22.792282705199977
26	12.883033753861039	38.99626256665444	27.527711765737887	20.59299191374663
27	15.478928425227409	38.274932614555254	30.192613385646766	16.053525574570568
28	10.806126626155654	30.6704519476653	43.70829944124574	14.815121984933304
29	10.909158572438688	23.346783840252634	43.81696677777955	21.927090809529126
30	15.412154367161623	32.51162963789959	34.42106997985082	17.65514601508796
31	31.3774276038427	26.960876569004288	23.60686241673179	18.05483341042122
32	31.707363753807876	28.202576330297664	25.4909966666135	14.599063249280956
33	28.662977080974176	31.4724849412803	23.25991376789635	16.604624209849174
34	17.69905952779683	31.09512645071426	27.882528695300827	23.323285326188085
35	17.33020728666592	28.15398438039948	30.9746566930892	23.5411516398454
36	32.84305438151592	22.14602040436583	28.31772968202576	16.693195532092485
37	18.7913682833857	31.318202842150594	33.117699910152744	16.772728964310968
38	18.48152814771102	32.94098257813788	23.56592609132522	25.01156318282588
39	18.21847238392957	32.1887111437184	30.385279935352504	19.207536536999527
40	22.760915910407927	28.290403355715405	23.713190534670943	25.235490199205728
41	15.815137934150997	22.688931774563123	29.84364450257048	31.652285788715396
42	23.798572013376077	23.444393052520773	24.65440703466828	28.102627899434868
43	24.11681207036795	25.86601593858488	25.515452133739508	24.501719857307666
44	18.970850146466983	33.31440692834016	26.373520045508435	21.34122287968442
45	14.300068581636072	44.205808705083015	19.60424674503049	21.889875968250426
46	23.898839428592694	34.985884942343574	24.250466514617457	16.86480911444627
47	21.57567637974024	30.62175366964917	25.206462623008342	22.596107327602247
48	25.331185505350962	23.246729081271898	29.595262019064634	21.82682339431251
49	22.409076168147283	21.089437896404515	32.7981839157456	23.703302019702598
50	19.041452016778575	33.71898541709862	26.940886882831734	20.298675683291066
51	16.657043971993176	37.78273975661494	21.072106413180432	24.488109858211455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	556.0
1	647.0
2	738.0
3	2608.0
4	4478.0
5	3535.5
6	2593.0
7	2748.0
8	2903.0
9	3202.5
10	3502.0
11	3616.5
12	3731.0
13	3597.0
14	3463.0
15	3075.5
16	2688.0
17	2522.5
18	2357.0
19	2280.0
20	2203.0
21	2238.5
22	2274.0
23	2498.0
24	2722.0
25	3364.5
26	4420.0
27	4833.0
28	6025.5
29	7218.0
30	9000.0
31	10782.0
32	13215.5
33	15649.0
34	16804.5
35	17960.0
36	20089.5
37	22219.0
38	28515.0
39	34811.0
40	55018.5
41	75226.0
42	104938.0
43	134650.0
44	133184.0
45	131718.0
46	122797.5
47	113877.0
48	100940.0
49	88003.0
50	80262.5
51	72522.0
52	63241.0
53	53960.0
54	45495.5
55	37031.0
56	32475.0
57	27919.0
58	23697.0
59	19475.0
60	16306.5
61	13138.0
62	11173.5
63	9209.0
64	7347.5
65	5486.0
66	4476.0
67	3466.0
68	2329.5
69	1193.0
70	1122.0
71	1051.0
72	741.0
73	431.0
74	289.0
75	137.5
76	128.0
77	85.5
78	43.0
79	55.5
80	68.0
81	41.5
82	15.0
83	11.5
84	8.0
85	8.0
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	5.0
94	7.0
95	6.5
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	940485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.199494351206948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61695046607358	15.035867233212501
2	7.504660742582055	2.7316206158469334
3	2.1603460714401153	1.179516183714795
4	0.939659592932758	0.6840531781454862
5	0.5482066202520833	0.49885416442860214
6	0.3696187227049105	0.40361243135810076
7	0.27064304606491507	0.34478966116363047
8	0.17637362946006135	0.25679286984482036
9	0.16321219793688835	0.26733415269604227
>10	2.0136655114479303	9.437539289546319
>50	1.486402484914939	20.489450869472922
>100	1.7455700976693387	47.38723747819007
>500	0.0035181123903312173	0.38630146166762314
>1k	5.863520650552028E-4	0.3079740382245194
>5k	5.863520650552028E-4	0.5890563724876463
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5520	0.5869312110240993	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2886	0.3068629483723823	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04263757529359852	0.0
2	0.0	0.0	0.0	0.16991233246675916	0.0
3	0.0	0.0	0.0	0.24349139008064988	0.0
4	0.0	0.0	0.0	0.3858647400011696	0.0
5	0.0	0.0	0.0	0.6722063616112963	0.0
6	0.0	0.0	0.0	0.925586266660287	0.0
7	0.0	0.0	0.0	1.074658288010973	0.0
8	0.0	0.0	0.0	1.5258084924267798	0.0
9	0.0	0.0	0.0	1.6640350457476727	0.0
10	0.0	0.0	0.0	1.965049947633402	0.0
11	0.0	0.0	0.0	2.2564953189046077	0.0
12	0.0	0.0	0.0	2.5280573321211928	0.0
13	0.0	0.0	0.0	2.634279121942402	0.0
14	0.0	0.0	0.0	2.666283885442086	0.0
15	0.0	0.0	0.0	2.72944278749794	0.0
16	0.0	0.0	0.0	2.8772388714333563	0.0
17	0.0	0.0	0.0	3.0592726093451783	0.0
18	0.0	0.0	0.0	3.3659229014816825	0.0
19	0.0	0.0	0.0	3.4776737534357274	0.0
20	0.0	0.0	0.0	3.6033535888398007	0.0
21	0.0	0.0	0.0	3.766248265522576	0.0
22	0.0	0.0	0.0	3.9325454419794044	0.0
23	0.0	0.0	0.0	4.116280429778253	0.0
24	0.0	0.0	0.0	4.236218546813612	0.0
25	0.0	0.0	0.0	4.337017602619925	0.0
26	0.0	0.0	0.0	4.4536595479991705	0.0
27	0.0	0.0	0.0	4.540210636001637	0.0
28	0.0	0.0	0.0	4.644624847817881	0.0
29	0.0	0.0	0.0	4.761266793197127	0.0
30	0.0	0.0	0.0	4.92065264198791	0.0
31	0.0	0.0	0.0	5.083759974906564	0.0
32	0.0	0.0	0.0	5.206887935480098	0.0
33	0.0	0.0	0.0	5.322572927797892	0.0
34	0.0	0.0	0.0	5.457928621934427	0.0
35	0.0	0.0	0.0	5.6428332190306065	0.0
36	0.0	0.0	0.0	5.787864771899605	0.0
37	0.0	0.0	0.0	5.9343849184197515	0.0
38	0.0	0.0	0.0	6.06070272253146	0.0
39	0.0	0.0	0.0	6.193293885601578	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	90	0.0	45.000004	1
TTAGGCG	60	0.0	45.000004	1
GCCCATA	30	2.1650794E-6	45.000004	24
ATTAGCG	45	3.8380676E-10	45.000004	1
TAACGAG	30	2.1650794E-6	45.000004	1
ATGTACG	30	2.1650794E-6	45.000004	1
GCTTAAG	65	0.0	45.000004	1
AACGCCG	30	2.1650794E-6	45.000004	13
CGGGACG	30	2.1650794E-6	45.000004	6
GAACGCT	30	2.1650794E-6	45.000004	30
ACTCGAC	30	2.1650794E-6	45.000004	10
CGGTCTA	115	0.0	45.000004	31
ACGTAAG	45	3.8380676E-10	45.000004	1
CGTCCTT	30	2.1650794E-6	45.000004	12
CGATCAT	30	2.1650794E-6	45.000004	10
TCGACTG	30	2.1650794E-6	45.000004	12
TATGTCG	30	2.1650794E-6	45.000004	1
AAGTGCG	65	0.0	45.000004	1
TACCGAT	30	2.1650794E-6	45.000004	3
ACGGTCT	115	0.0	45.000004	30
>>END_MODULE