FastQCFastQC Report
Thu 26 May 2016
SRR1548062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548062_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2990792
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426011.4244053080254326No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52540.17567253088813933No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.13912702722222076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220950.043.645621
CACGACG3350.042.31343526
CGGTTTT20850.040.25181
CGACGGT3250.040.15384728
TCACGAC3450.039.78260825
TATAGCG1700.039.7058831
CGTTAGG4650.039.677422
TACGGGA9800.039.4897964
GCGGGTC2450.039.4897965
CGGGACC7550.039.3377466
CGGTCTA3450.039.13043631
CGAGGGA16150.038.591334
GCGTAAG2050.038.4146351
GGCGATA9000.038.258
AAGGGAT50000.038.0255
GGCGTAG2200.037.8409081
TATCCGG1250.037.82
GGGCGAT34200.037.7631577
AGGGCGA17950.037.479116
GCGGGAT20300.037.4630555