##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548062_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2990792
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.467480185850437	31.0	31.0	33.0	30.0	34.0
2	31.850666311799685	31.0	31.0	34.0	30.0	34.0
3	31.681586014674373	31.0	31.0	34.0	30.0	34.0
4	35.529154150472515	37.0	35.0	37.0	33.0	37.0
5	35.52410799547411	37.0	35.0	37.0	33.0	37.0
6	35.63109069437126	37.0	35.0	37.0	33.0	37.0
7	36.04736638321889	37.0	35.0	37.0	35.0	37.0
8	36.090188819550136	37.0	35.0	37.0	35.0	37.0
9	37.905153551300124	39.0	38.0	39.0	35.0	39.0
10	37.32300808615243	39.0	37.0	39.0	34.0	39.0
11	36.96441678324671	39.0	37.0	39.0	33.0	39.0
12	36.49303060861471	39.0	35.0	39.0	32.0	39.0
13	36.31331801074766	39.0	35.0	39.0	32.0	39.0
14	37.403084868489685	40.0	35.0	41.0	32.0	41.0
15	37.58377078713598	40.0	35.0	41.0	33.0	41.0
16	37.67770309670482	40.0	35.0	41.0	33.0	41.0
17	37.61177273444626	40.0	35.0	41.0	33.0	41.0
18	37.452444034891094	39.0	36.0	41.0	32.0	41.0
19	37.30886735018684	39.0	36.0	41.0	32.0	41.0
20	37.1069021182349	39.0	35.0	41.0	32.0	41.0
21	36.98217328386595	38.0	35.0	41.0	32.0	41.0
22	36.91936985253405	38.0	35.0	41.0	32.0	41.0
23	36.82380921174057	38.0	35.0	41.0	32.0	41.0
24	36.81086982979759	38.0	35.0	41.0	32.0	41.0
25	36.77405249178144	38.0	35.0	41.0	32.0	41.0
26	36.6569758110895	38.0	35.0	41.0	31.0	41.0
27	36.587250467434714	38.0	35.0	40.0	31.0	41.0
28	36.516449488964795	38.0	35.0	40.0	31.0	41.0
29	36.456712803832566	38.0	35.0	40.0	31.0	41.0
30	36.3469398741203	38.0	35.0	40.0	31.0	41.0
31	36.18846044793486	38.0	35.0	40.0	30.0	41.0
32	35.94218120150114	38.0	35.0	40.0	30.0	41.0
33	35.68416827382178	38.0	35.0	40.0	29.0	41.0
34	35.3907707389882	38.0	34.0	40.0	26.0	41.0
35	35.13333993136266	38.0	34.0	40.0	25.0	41.0
36	34.92943073272899	38.0	34.0	40.0	24.0	41.0
37	34.81409539680459	38.0	34.0	40.0	23.0	41.0
38	34.70851600512506	38.0	34.0	40.0	23.0	41.0
39	34.648030354501415	38.0	34.0	40.0	23.0	41.0
40	34.533366412642536	38.0	34.0	40.0	23.0	41.0
41	34.47600334627082	38.0	34.0	40.0	22.0	41.0
42	34.41592862358867	38.0	34.0	40.0	22.0	41.0
43	34.29886397984213	37.0	33.0	40.0	22.0	41.0
44	34.12714525115755	37.0	33.0	40.0	21.0	41.0
45	34.09856853970453	37.0	33.0	40.0	21.0	41.0
46	34.029367806253326	37.0	33.0	40.0	20.0	41.0
47	33.96393697722878	37.0	33.0	40.0	20.0	41.0
48	33.83077358773195	37.0	33.0	40.0	20.0	41.0
49	33.72154432672015	36.0	33.0	40.0	20.0	41.0
50	33.56033518880618	36.0	33.0	40.0	19.0	41.0
51	33.204759475082184	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	8.0
13	13.0
14	40.0
15	61.0
16	199.0
17	501.0
18	1189.0
19	2597.0
20	4545.0
21	7350.0
22	12109.0
23	18440.0
24	28614.0
25	44544.0
26	61668.0
27	67987.0
28	66617.0
29	67863.0
30	74469.0
31	86954.0
32	105489.0
33	134034.0
34	219233.0
35	331888.0
36	205393.0
37	259242.0
38	406264.0
39	783335.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.258303486166877	23.84652627130205	29.699959074385646	17.195211168145427
2	34.93382354907998	24.621404631281614	28.040331791712696	12.404440027925713
3	27.217773753574303	24.388824097429712	35.5139374453322	12.879464703663778
4	24.09338395983405	26.434469531816323	35.77771372933992	13.694432779009707
5	21.724513105558664	31.0166337211013	33.90941262381336	13.349440549526681
6	19.719091130376167	39.54220153056448	30.729184777811362	10.00952256124799
7	85.03286086093583	2.9814176311826435	10.526709981837588	1.4590115260439376
8	85.91941532543889	2.008364339613052	10.369494100559317	1.7027262343887506
9	81.35009054457815	4.7355014992684215	11.762603350550624	2.151804605602797
10	42.0124502138564	31.886436769925826	17.138269729222227	8.962843286995552
11	30.170235843883493	22.329302739876262	31.825984555261616	15.674476860978631
12	29.608879520876073	20.906034254471724	32.65960989597404	16.825476328678157
13	21.732504299864384	29.558859325556575	32.33671883567965	16.371917538899396
14	16.556082803484827	32.44291144285527	33.86892167693373	17.132084076726166
15	15.394250084927336	26.603153947181884	42.68735505511583	15.315240912774945
16	19.095577358773195	24.427944169972367	40.52264416916991	15.953834302084532
17	19.145396938336066	24.446267075744483	32.481429668128044	23.926906317791406
18	20.584146272960474	25.542364698046537	35.881498947436	17.99199008155699
19	22.90062297879625	27.43704677556982	31.078557117980786	18.583773127653146
20	24.309079334169677	26.905849687975625	31.15693100690386	17.628139970950837
21	21.517210157042012	26.971317296555565	33.66486201648259	17.846610529919836
22	21.299040521707962	23.664534344080096	33.13446739191492	21.901957742297025
23	18.217315012210815	27.840551934069634	32.735275472182614	21.20685758153693
24	18.599855824142903	26.119201870273827	37.31683781419771	17.96410449138556
25	17.96938737297679	27.42908901722353	34.821879956880984	19.77964365291869
26	17.759877651137224	31.26827275183296	31.449194728352893	19.522654868676923
27	17.855036391698253	29.73707298936202	34.26390066577682	18.143989953162908
28	16.06136434763768	27.959450205831764	37.96215183135437	18.01703361517618
29	17.20102902508767	25.577840251010436	37.586833186660925	19.634297537240972
30	18.60717829925986	27.413708475881975	35.435062017017565	18.5440512078406
31	22.853478275988433	26.404477476200285	31.258509451677014	19.48353479613427
32	23.18405960695361	27.330118577286548	31.393055752456206	18.092766063303635
33	22.204653483090766	27.869239987267587	30.937290189354528	18.98881634028712
34	18.569462537013607	27.835068436721777	32.51493249948509	21.08053652677953
35	18.115970619153725	28.637364283440643	32.57912954160637	20.667535555799265
36	22.785904201963895	27.138129298192588	30.980054781475943	19.095911718367574
37	19.341365096603173	30.270543722198003	31.353434140521973	19.03465704067685
38	18.676524479134624	30.931004228980154	29.546621764402204	20.845849527483022
39	18.871656738415776	30.067420268611123	30.765596537639528	20.29532645533357
40	20.369153053773047	28.307083876110404	29.935415100749236	21.38834796936731
41	17.826916749810753	27.022006211063825	31.201233653159434	23.94984338596599
42	20.333577192930836	27.96409780419367	29.508471334683257	22.193853668192236
43	20.622998857827625	28.277961155439762	29.688490540298357	21.410549446434253
44	19.514028391141878	30.555551840448953	29.725738199112477	20.204681569296696
45	18.180167661275007	31.99533768981594	28.223728029231054	21.600766619677998
46	20.547232973740734	30.32935757484974	29.194741727274913	19.92866772413461
47	19.979122586926806	28.3316927422569	30.19735240698785	21.491832263828446
48	20.583544425690587	27.270936929080992	31.11282897640491	21.032689668823508
49	19.95260787109234	26.422198534702513	31.390681799336097	22.234511794869054
50	18.30832769380151	29.9038515550396	30.608480964239572	21.179339786919318
51	18.08644666696982	30.579893218919942	29.145624302860245	22.188035811249996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1443.0
1	2550.0
2	3657.0
3	34874.5
4	66092.0
5	47784.0
6	29476.0
7	26984.5
8	24493.0
9	25294.0
10	26095.0
11	26758.0
12	27421.0
13	26879.0
14	26337.0
15	25258.5
16	24180.0
17	22893.0
18	21606.0
19	20743.0
20	19880.0
21	19557.5
22	19235.0
23	20464.0
24	21693.0
25	24278.5
26	30947.0
27	35030.0
28	41588.5
29	48147.0
30	56237.5
31	64328.0
32	73435.0
33	82542.0
34	90877.0
35	99212.0
36	110318.0
37	121424.0
38	134491.0
39	147558.0
40	170784.5
41	194011.0
42	214849.5
43	235688.0
44	242662.5
45	249637.0
46	253840.5
47	258044.0
48	253433.5
49	248823.0
50	232513.0
51	216203.0
52	193454.0
53	170705.0
54	151202.5
55	131700.0
56	120107.5
57	108515.0
58	94859.5
59	81204.0
60	70183.0
61	59162.0
62	51460.5
63	43759.0
64	34506.5
65	25254.0
66	19679.5
67	14105.0
68	10903.5
69	7702.0
70	6092.0
71	4482.0
72	3382.5
73	2283.0
74	1736.0
75	933.0
76	677.0
77	533.0
78	389.0
79	292.5
80	196.0
81	153.0
82	110.0
83	100.0
84	90.0
85	73.0
86	56.0
87	46.0
88	36.0
89	25.5
90	15.0
91	12.0
92	9.0
93	22.0
94	35.0
95	17.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2990792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.874276149912433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40333573050889	19.751005001848867
2	7.583474741476355	3.7726688979073733
3	2.7788708037121594	2.073671992693961
4	1.514673203679768	1.5070559818081244
5	1.007829678415428	1.2534516866491399
6	0.6975945408934745	1.04112955505134
7	0.5282041129325593	0.9197086478022809
8	0.4283786834282134	0.8524487734663438
9	0.32791965529087164	0.7341087654620312
>10	3.8198074633343815	22.182461109481714
>50	1.3187410730038793	23.52086441075882
>100	0.5880436377320373	19.984292496704263
>500	0.002175078672672419	0.3683434423513621
>1k	6.797120852101309E-4	0.42058714183110635
>5k	1.359424170420262E-4	0.17766239292335045
>10k+	1.359424170420262E-4	1.4405397032599263
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42601	1.4244053080254326	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5254	0.17567253088813933	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4161	0.13912702722222076	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3374383775267555E-4	0.0	0.0	0.04654285553793109	0.0
2	1.3374383775267555E-4	0.0	0.0	0.13397788946874273	0.0
3	1.3374383775267555E-4	0.0	0.0	0.23883305826684034	0.0
4	1.3374383775267555E-4	0.0	0.0	0.3455606407934754	0.0
5	1.3374383775267555E-4	0.0	0.0	0.6641050263609104	0.0
6	1.3374383775267555E-4	0.0	0.0	1.197742938994086	0.0
7	1.3374383775267555E-4	0.0	0.0	1.5775754382116844	0.0
8	1.3374383775267555E-4	0.0	0.0	2.3259725183162185	0.0
9	1.3374383775267555E-4	0.0	0.0	2.7273043394525596	0.0
10	1.3374383775267555E-4	0.0	0.0	3.23656743765531	0.0
11	1.3374383775267555E-4	0.0	0.0	3.6321817097277243	0.0
12	1.3374383775267555E-4	0.0	0.0	3.9578479546554894	0.0
13	1.3374383775267555E-4	0.0	0.0	4.126030830629479	0.0
14	1.3374383775267555E-4	0.0	0.0	4.2128640172904035	0.0
15	1.3374383775267555E-4	0.0	0.0	4.2952836573054896	0.0
16	1.3374383775267555E-4	0.0	0.0	4.449155942639943	0.0
17	1.3374383775267555E-4	0.0	0.0	4.648300517053677	0.0
18	1.3374383775267555E-4	0.0	0.0	4.917727478206442	0.0
19	1.3374383775267555E-4	0.0	0.0	5.067253088813933	0.0
20	1.3374383775267555E-4	0.0	0.0	5.233128883586689	0.0
21	1.3374383775267555E-4	0.0	0.0	5.43177191860885	0.0
22	1.3374383775267555E-4	0.0	0.0	5.64158256408336	0.0
23	1.3374383775267555E-4	0.0	0.0	5.882689267591996	0.0
24	1.3374383775267555E-4	0.0	0.0	6.059164261506651	0.0
25	1.3374383775267555E-4	0.0	0.0	6.217617273284134	0.0
26	1.3374383775267555E-4	0.0	0.0	6.371723610334654	0.0
27	1.3374383775267555E-4	0.0	0.0	6.5399733582275195	0.0
28	1.6717979719084444E-4	0.0	0.0	6.7127035246851	0.0
29	1.6717979719084444E-4	0.0	0.0	6.897671252297051	0.0
30	1.6717979719084444E-4	0.0	0.0	7.13780831298198	0.0
31	1.6717979719084444E-4	0.0	0.0	7.346214648160086	0.0
32	1.6717979719084444E-4	0.0	0.0	7.542149370467755	0.0
33	1.6717979719084444E-4	0.0	0.0	7.73891999176138	0.0
34	1.6717979719084444E-4	0.0	0.0	7.948095354006564	0.0
35	1.6717979719084444E-4	0.0	0.0	8.183417636532397	0.0
36	1.6717979719084444E-4	0.0	0.0	8.401821323582515	0.0
37	1.6717979719084444E-4	0.0	0.0	8.632997547138016	0.0
38	1.6717979719084444E-4	3.343595943816889E-5	0.0	8.871997785202046	0.0
39	1.6717979719084444E-4	3.343595943816889E-5	0.0	9.159012061019288	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22095	0.0	43.64562	1
CACGACG	335	0.0	42.313435	26
CGGTTTT	2085	0.0	40.2518	1
CGACGGT	325	0.0	40.153847	28
TCACGAC	345	0.0	39.782608	25
TATAGCG	170	0.0	39.705883	1
CGTTAGG	465	0.0	39.67742	2
TACGGGA	980	0.0	39.489796	4
GCGGGTC	245	0.0	39.489796	5
CGGGACC	755	0.0	39.337746	6
CGGTCTA	345	0.0	39.130436	31
CGAGGGA	1615	0.0	38.59133	4
GCGTAAG	205	0.0	38.414635	1
GGCGATA	900	0.0	38.25	8
AAGGGAT	5000	0.0	38.025	5
GGCGTAG	220	0.0	37.840908	1
TATCCGG	125	0.0	37.8	2
GGGCGAT	3420	0.0	37.763157	7
AGGGCGA	1795	0.0	37.47911	6
GCGGGAT	2030	0.0	37.463055	5
>>END_MODULE