FastQCFastQC Report
Thu 26 May 2016
SRR1548061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548061_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1535170
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118790.7737905248278693No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18740.12207117127093416No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.034027E-445.01
CGTCGTT207.034027E-445.034
TCGCGCC253.89142E-545.040
CGTTTTT72250.043.474051
CGACGGT1600.042.187528
CACGACG1550.042.0967726
ACGGGAC4850.041.752585
CGCGTAA556.184564E-1140.9090931
ATTCGCG501.0822987E-940.51
AGGGCGA9400.040.2127656
TACGGGA6550.040.1908384
GGCGATA4650.039.6774188
GGGCGAT19200.039.023447
TGGGCGA5700.038.684216
CGTCCTA356.24966E-638.57142625
TAGGGCG4450.038.4269685
CGTTAGG2050.038.4146352
CGAGGGT1700.038.382354
CGAGGGA13000.038.254
CTCACGT1650.038.18181645