##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548061_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1535170
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49452633910251	31.0	31.0	33.0	30.0	34.0
2	31.896195209651047	31.0	31.0	34.0	30.0	34.0
3	31.870293192284894	33.0	31.0	34.0	30.0	34.0
4	35.64756281063335	37.0	35.0	37.0	33.0	37.0
5	35.59149670720507	37.0	35.0	37.0	33.0	37.0
6	35.67268315561143	37.0	35.0	37.0	33.0	37.0
7	36.05453141997303	37.0	35.0	37.0	35.0	37.0
8	36.08849117687292	37.0	35.0	37.0	35.0	37.0
9	37.89647921728538	39.0	38.0	39.0	35.0	39.0
10	37.33371092452302	39.0	37.0	39.0	34.0	39.0
11	36.99774487516041	39.0	37.0	39.0	33.0	39.0
12	36.516071835692465	39.0	35.0	39.0	32.0	39.0
13	36.32545516131764	39.0	35.0	39.0	32.0	39.0
14	37.38967150217891	40.0	35.0	41.0	32.0	41.0
15	37.583650019216115	40.0	35.0	41.0	32.0	41.0
16	37.67349935186331	40.0	35.0	41.0	33.0	41.0
17	37.595916413165966	40.0	35.0	41.0	33.0	41.0
18	37.470130343870714	39.0	36.0	41.0	32.0	41.0
19	37.398109655608174	39.0	36.0	41.0	32.0	41.0
20	37.24781750555313	39.0	35.0	41.0	32.0	41.0
21	37.138711022232066	39.0	35.0	41.0	32.0	41.0
22	37.077388823387636	39.0	35.0	41.0	32.0	41.0
23	36.96681735573259	39.0	35.0	41.0	32.0	41.0
24	36.966812144583336	39.0	35.0	41.0	32.0	41.0
25	36.92258316668512	39.0	35.0	41.0	32.0	41.0
26	36.81554160125589	39.0	35.0	41.0	31.0	41.0
27	36.75530136727529	39.0	35.0	41.0	31.0	41.0
28	36.693022271149125	38.0	35.0	41.0	31.0	41.0
29	36.63151507650618	38.0	35.0	41.0	31.0	41.0
30	36.536700170013745	38.0	35.0	41.0	31.0	41.0
31	36.413819967821155	38.0	35.0	41.0	30.0	41.0
32	36.2645772129471	38.0	35.0	41.0	30.0	41.0
33	36.099613072167905	38.0	35.0	41.0	30.0	41.0
34	35.922021013959366	38.0	35.0	41.0	29.0	41.0
35	35.748393988939334	38.0	35.0	41.0	28.0	41.0
36	35.57683969853502	38.0	35.0	41.0	27.0	41.0
37	35.47411231329429	38.0	35.0	40.0	27.0	41.0
38	35.36089683878659	38.0	34.0	40.0	26.0	41.0
39	35.31109714233603	38.0	34.0	40.0	26.0	41.0
40	35.1867708462255	38.0	34.0	40.0	25.0	41.0
41	35.12656643889602	38.0	34.0	40.0	24.0	41.0
42	35.09147716539536	38.0	34.0	40.0	24.0	41.0
43	34.9977253333507	38.0	34.0	40.0	24.0	41.0
44	34.83686432121524	38.0	34.0	40.0	24.0	41.0
45	34.81597021828201	38.0	34.0	40.0	24.0	41.0
46	34.75381228137601	38.0	34.0	40.0	24.0	41.0
47	34.67316453552375	37.0	34.0	40.0	23.0	41.0
48	34.553598624256594	37.0	34.0	40.0	23.0	41.0
49	34.45525055857006	37.0	34.0	40.0	24.0	41.0
50	34.30803168378746	37.0	33.0	40.0	23.0	41.0
51	33.94506276177883	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	5.0
13	14.0
14	15.0
15	40.0
16	110.0
17	295.0
18	666.0
19	1255.0
20	2277.0
21	3685.0
22	5553.0
23	8337.0
24	12245.0
25	18245.0
26	24509.0
27	27923.0
28	29427.0
29	31588.0
30	35879.0
31	42814.0
32	52479.0
33	66895.0
34	110402.0
35	163378.0
36	105203.0
37	137216.0
38	222712.0
39	431931.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.42296944312356	23.91396392581929	29.52572027853593	15.13734635252122
2	32.052736830448744	25.29061927994945	29.55340450894689	13.10323938065491
3	28.400112039708958	25.394646846929003	32.829067790537856	13.376173322824183
4	25.30599217024825	27.292026290247982	32.74321410658103	14.658767432922737
5	23.156588521140982	32.098790361979454	31.175960968492088	13.568660148387476
6	21.27178097539686	40.96184787352541	27.70168776096458	10.064683390113146
7	88.14320238149521	3.12577760117772	7.367522815062827	1.3634972022642444
8	89.15462131229766	2.1787163636600506	7.17060651263378	1.4960558114085085
9	84.3430369275064	5.138779418566021	8.411967404261416	2.1062162496661605
10	40.711452151878945	36.531459056651705	13.61621188532866	9.140876906140688
11	31.50094126383397	22.033064742015544	30.1055909117557	16.360403082394782
12	30.571728212510667	21.61552140805253	30.61693493228763	17.195815447149176
13	23.360214178234333	29.306005198121383	30.54834318023411	16.785437443410174
14	17.143443397148197	33.06591452412436	32.124064435860525	17.666577642866912
15	16.49602324172567	27.292938241367402	40.28498472481875	15.926053792088174
16	21.09525329442342	24.555847235159625	37.83183621357895	16.517063256838004
17	20.945367614010173	24.019033722649606	30.834304995537952	24.20129366780226
18	22.25551567578835	25.58674283629826	33.30894949744979	18.8487919904636
19	24.216601418735383	26.757166958708154	30.089696906531525	18.936534716024937
20	25.72386120103962	27.203567031664242	29.102770377222065	17.969801390074064
21	23.218210361067506	26.400528931649266	31.885654357497867	18.495606349785366
22	22.895119107330135	22.988789515167703	31.988704833992326	22.12738654350984
23	19.285160601106067	27.522815062826915	31.58959594051473	21.602428395552284
24	19.65554303432193	26.284255163923216	35.350026381443094	18.710175420311757
25	19.00408423822769	28.018395356866016	33.13392002188682	19.84360038301947
26	19.150843229088636	31.095969827445817	29.252395500172618	20.500791443292925
27	18.752125171805076	29.620954031149644	32.59547802523499	19.03144277181029
28	16.980790401063075	28.461668740269808	35.84182859227317	18.71571226639395
29	18.329044991759872	26.194883954220057	34.80383280027619	20.672238253743885
30	20.34927727873786	26.983265696958643	33.14023854035709	19.527218483946402
31	24.158757662017887	26.014513050671912	29.54369874346164	20.283030543848565
32	23.642267631597804	27.415204830735355	29.897926613990634	19.044600923676207
33	23.077053355654424	27.027169629422147	30.34589003172287	19.549886983200558
34	19.65391455018011	28.029729606493092	31.221688803194436	21.094667040132364
35	19.340333643830977	27.83085912309386	31.720851762345536	21.107955470729625
36	23.94386289466313	27.250793071777068	29.733905691226383	19.071438342333423
37	20.60944390523525	29.48513845372174	30.49903268041976	19.406384960623253
38	20.327781288065815	30.58071744497352	27.854374434101757	21.237126832858902
39	19.858777855221245	29.434460027228255	29.97902512425334	20.72773699329716
40	21.67662213305367	27.882319221975415	29.09084987330393	21.35020877166698
41	18.9543829022193	27.198030185582052	29.996156777425302	23.851430134773345
42	21.335747832487606	27.662408723463848	28.349107916387112	22.652735527661434
43	21.258687962896616	28.05800009119511	28.851853540650218	21.83145840525805
44	20.21639297276523	30.13698808601002	29.143808177595965	20.502810763628784
45	19.124722343453822	31.327931108606865	27.933127927200246	21.61421862073907
46	21.090563260094974	29.564999316036662	28.63331096881779	20.71112645505058
47	20.82485978751539	28.521531817323165	29.7516235986894	20.90198479647205
48	21.3230456561814	26.612883263742777	30.60299510803364	21.461075972042185
49	20.557853527622346	25.798706332197735	31.40277623976498	22.240663900414937
50	19.247770605209848	29.697557925180924	29.961046659327632	21.093624810281597
51	18.814854380948038	30.317684686386524	28.76313372460379	22.104327208061648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	582.0
1	1234.0
2	1886.0
3	11092.5
4	20299.0
5	14816.5
6	9334.0
7	8730.5
8	8127.0
9	8301.5
10	8476.0
11	8547.5
12	8619.0
13	8459.0
14	8299.0
15	7782.0
16	7265.0
17	7399.5
18	7534.0
19	7443.0
20	7352.0
21	7637.5
22	7923.0
23	9011.5
24	10100.0
25	12106.0
26	16725.5
27	19339.0
28	23064.5
29	26790.0
30	30403.5
31	34017.0
32	37615.0
33	41213.0
34	48556.5
35	55900.0
36	59504.5
37	63109.0
38	69536.0
39	75963.0
40	87408.5
41	98854.0
42	113507.5
43	128161.0
44	131748.0
45	135335.0
46	134303.0
47	133271.0
48	128410.0
49	123549.0
50	115079.5
51	106610.0
52	99343.0
53	92076.0
54	82127.0
55	72178.0
56	65038.5
57	57899.0
58	53146.0
59	48393.0
60	43090.5
61	37788.0
62	31532.0
63	25276.0
64	20799.5
65	16323.0
66	13242.5
67	10162.0
68	7834.0
69	5506.0
70	4252.0
71	2998.0
72	2534.5
73	2071.0
74	1587.5
75	897.5
76	691.0
77	499.5
78	308.0
79	249.5
80	191.0
81	142.5
82	94.0
83	75.0
84	56.0
85	37.0
86	18.0
87	14.5
88	11.0
89	7.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1535170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.22030493242444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1153357758482	19.9531289309804
2	6.246665501225578	3.1508561750353006
3	2.292327047317004	1.7343956141453696
4	1.3009094159752856	1.3123732864143558
5	0.8982179985146506	1.1326665909165725
6	0.6817808497687244	1.0316832556952815
7	0.5967096530476358	1.0534439584187796
8	0.475794140145408	0.9599738639622303
9	0.4056445027308681	0.9207430247730755
>10	6.433127085907545	40.58948724844368
>50	1.4706380538764672	24.477130167662754
>100	0.0820732571256072	2.7097571514593266
>500	0.0	0.0
>1k	5.178123447000217E-4	0.1986985462913116
>5k	0.0	0.0
>10k+	2.5890617235001083E-4	0.7756621858016728
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11879	0.7737905248278693	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1874	0.12207117127093416	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9541809701857123E-4	0.0	0.0	0.01719679253763427	0.0
2	1.9541809701857123E-4	0.0	0.0	0.06227323358325137	0.0
3	1.9541809701857123E-4	0.0	0.0	0.1113883153005856	0.0
4	1.9541809701857123E-4	0.0	0.0	0.17880755877199267	0.0
5	1.9541809701857123E-4	0.0	0.0	0.3460203104542168	0.0
6	1.9541809701857123E-4	0.0	0.0	0.6093136265039051	0.0
7	1.9541809701857123E-4	0.0	0.0	0.7990645987089378	0.0
8	1.9541809701857123E-4	0.0	0.0	1.254258486030863	0.0
9	1.9541809701857123E-4	0.0	0.0	1.4942319091696685	0.0
10	1.9541809701857123E-4	0.0	0.0	1.828527133802771	0.0
11	1.9541809701857123E-4	0.0	0.0	2.095207696867448	0.0
12	1.9541809701857123E-4	0.0	0.0	2.329839692021079	0.0
13	1.9541809701857123E-4	0.0	0.0	2.4369288091872563	0.0
14	1.9541809701857123E-4	0.0	0.0	2.487998071874776	0.0
15	1.9541809701857123E-4	0.0	0.0	2.5475354520997673	0.0
16	1.9541809701857123E-4	0.0	0.0	2.6513024616166287	0.0
17	1.9541809701857123E-4	0.0	0.0	2.793762254343167	0.0
18	1.9541809701857123E-4	0.0	0.0	2.9722441162867956	0.0
19	1.9541809701857123E-4	0.0	0.0	3.075880847072311	0.0
20	1.9541809701857123E-4	0.0	0.0	3.189093064611737	0.0
21	1.9541809701857123E-4	0.0	0.0	3.332725365920387	0.0
22	1.9541809701857123E-4	0.0	0.0	3.491079163871102	0.0
23	1.9541809701857123E-4	0.0	0.0	3.6650664095833037	0.0
24	1.9541809701857123E-4	0.0	0.0	3.7922184513767205	0.0
25	1.9541809701857123E-4	0.0	0.0	3.9074499892520045	0.0
26	1.9541809701857123E-4	0.0	0.0	4.018121771530189	0.0
27	1.9541809701857123E-4	0.0	0.0	4.133874424330856	0.0
28	1.9541809701857123E-4	0.0	0.0	4.258746588325723	0.0
29	1.9541809701857123E-4	0.0	0.0	4.398926503253711	0.0
30	1.9541809701857123E-4	0.0	0.0	4.576822110906284	0.0
31	1.9541809701857123E-4	0.0	0.0	4.728857390386732	0.0
32	1.9541809701857123E-4	0.0	0.0	4.871773158672981	0.0
33	1.9541809701857123E-4	0.0	0.0	5.021789117817571	0.0
34	1.9541809701857123E-4	0.0	0.0	5.184637531999713	0.0
35	1.9541809701857123E-4	0.0	0.0	5.363445090771706	0.0
36	1.9541809701857123E-4	0.0	0.0	5.524013627155298	0.0
37	1.9541809701857123E-4	0.0	0.0	5.6949393226808755	0.0
38	1.9541809701857123E-4	0.0	0.0	5.903971547125074	0.0
39	1.9541809701857123E-4	0.0	0.0	6.219376355713048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.034027E-4	45.0	1
CGTCGTT	20	7.034027E-4	45.0	34
TCGCGCC	25	3.89142E-5	45.0	40
CGTTTTT	7225	0.0	43.47405	1
CGACGGT	160	0.0	42.1875	28
CACGACG	155	0.0	42.09677	26
ACGGGAC	485	0.0	41.75258	5
CGCGTAA	55	6.184564E-11	40.90909	31
ATTCGCG	50	1.0822987E-9	40.5	1
AGGGCGA	940	0.0	40.212765	6
TACGGGA	655	0.0	40.190838	4
GGCGATA	465	0.0	39.677418	8
GGGCGAT	1920	0.0	39.02344	7
TGGGCGA	570	0.0	38.68421	6
CGTCCTA	35	6.24966E-6	38.571426	25
TAGGGCG	445	0.0	38.426968	5
CGTTAGG	205	0.0	38.414635	2
CGAGGGT	170	0.0	38.38235	4
CGAGGGA	1300	0.0	38.25	4
CTCACGT	165	0.0	38.181816	45
>>END_MODULE