FastQCFastQC Report
Thu 26 May 2016
SRR1548060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548060_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2363108
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.27036428296971615No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51700.21877967490271286No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC253.8920607E-545.024
CCGTGTA253.8920607E-545.02
CTAACGT207.0348004E-445.010
GTTCGAC253.8920607E-545.026
CGTCGGT253.8920607E-545.019
GAACCGT253.8920607E-545.03
ATACGTC207.0348004E-445.011
AATACGC207.0348004E-445.012
ATCGATT207.0348004E-445.032
TGCGTAT207.0348004E-445.01
ACGTTAG1000.042.751
TAATGCG1900.042.631581
CGGGTAT850.042.352946
CACGACC3000.042.027
GGTTACG1350.041.6666681
GACACGA3050.041.31147425
CGGGATC4850.041.288666
CGCATGG3450.041.0869562
GGCGATA7450.041.0738268
CGGTCTA2750.040.90909231
Produced by FastQC (version 0.11.5)