FastQCFastQC Report
Thu 26 May 2016
SRR1548059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548059_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3900924
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125650.3221031735045338No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC92810.23791799071194417No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG253.8925296E-545.019
TTACGTA253.8925296E-545.042
GTCGGTC253.8925296E-545.022
AATCGGC253.8925296E-545.029
TCTATCG406.8230293E-945.042
CGCATCG2400.042.18750421
CGGTCTA10050.041.64179231
AATCGCG603.6379788E-1241.2500041
ACGGTCT10400.040.24038330
CGACGGT10500.040.0714328
CGTTTTT75650.039.3787161
TCACGAC11050.039.09502425
TACGGGA12650.038.7747044
CGTAAGG7800.038.6538472
CTCACGA11200.038.5714324
CGTTTGG6300.038.571432
CGGGGTA3950.038.1645556
GGGCGAT49500.038.1363647
GCGAACG659.094947E-1238.076921
TAGGGAC36200.038.0386775
Produced by FastQC (version 0.11.5)