FastQCFastQC Report
Thu 26 May 2016
SRR1548058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548058_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2362717
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458961.9425094076015028No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54670.23138615416065486No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.18834248875341397No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT25840.10936561594130825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA351.2126657E-745.015
CGGCCTA207.034801E-445.027
CGTTTTT205150.044.133561
CGACGGT5450.043.34862528
GACGGGT2350.042.127664
TTGTACG1300.041.5384641
CGCATCG1100.040.90909221
ACGGGTA2200.040.9090925
TATAGCG1600.040.781251
TAGGGTA12700.040.748035
CGTTCGG1050.040.71428745
TACGGGA8800.040.6534084
CGAAAGG3500.040.5000042
CACGACG5850.040.026
AATGCGG3150.040.02
GTAGCGA1800.040.034
ATACGAA3950.039.87341719
ATTAGCG1750.039.8571431
TCTAGCG850.039.7058831
CGGTCTA5950.039.70588331
Produced by FastQC (version 0.11.5)