##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548058_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2362717
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.396091025713194	31.0	31.0	33.0	30.0	34.0
2	31.74873503682413	31.0	31.0	34.0	30.0	34.0
3	31.50233862117215	31.0	31.0	34.0	30.0	34.0
4	35.39580703063465	37.0	35.0	37.0	33.0	37.0
5	35.39898726762452	37.0	35.0	37.0	33.0	37.0
6	35.513952369242695	37.0	35.0	37.0	33.0	37.0
7	35.99211881913915	37.0	35.0	37.0	35.0	37.0
8	36.04516368231997	37.0	35.0	37.0	35.0	37.0
9	37.865063822709196	39.0	38.0	39.0	35.0	39.0
10	37.21913500431918	39.0	37.0	39.0	33.0	39.0
11	36.8682859606123	39.0	37.0	39.0	32.0	39.0
12	36.15282532779	38.0	35.0	39.0	32.0	39.0
13	35.837996679246814	38.0	35.0	39.0	31.0	39.0
14	36.822547093028916	39.0	35.0	41.0	31.0	41.0
15	37.06789386964245	39.0	35.0	41.0	32.0	41.0
16	37.223802088866336	39.0	35.0	41.0	32.0	41.0
17	37.17600626736084	39.0	35.0	41.0	32.0	41.0
18	37.01221390458527	38.0	35.0	40.0	32.0	41.0
19	36.83057259925755	38.0	35.0	40.0	32.0	41.0
20	36.554298293024516	38.0	35.0	40.0	32.0	41.0
21	36.41576794851013	37.0	35.0	40.0	31.0	41.0
22	36.3459424044437	37.0	35.0	40.0	31.0	41.0
23	36.27528646046056	37.0	35.0	40.0	31.0	41.0
24	36.24218135307783	37.0	35.0	40.0	31.0	41.0
25	36.189457306990214	37.0	35.0	40.0	31.0	41.0
26	36.041962283252715	37.0	35.0	40.0	31.0	41.0
27	35.966823788037246	37.0	35.0	40.0	31.0	41.0
28	35.91527804641859	37.0	35.0	40.0	30.0	41.0
29	35.83665373381577	36.0	35.0	40.0	30.0	41.0
30	35.71953983485961	36.0	35.0	40.0	30.0	41.0
31	35.505343212919705	36.0	34.0	40.0	30.0	41.0
32	35.20369303644914	36.0	34.0	40.0	28.0	41.0
33	34.951654810965515	36.0	34.0	40.0	26.0	41.0
34	34.63497194120159	36.0	34.0	40.0	24.0	41.0
35	34.3418208782516	36.0	34.0	40.0	23.0	41.0
36	34.085035152326746	36.0	33.0	40.0	21.0	41.0
37	33.940120209064396	36.0	33.0	40.0	21.0	41.0
38	33.80130544622991	36.0	33.0	40.0	20.0	41.0
39	33.767848201879445	36.0	33.0	40.0	20.0	41.0
40	33.6276468997345	36.0	33.0	40.0	18.0	41.0
41	33.56773451919972	36.0	33.0	40.0	18.0	41.0
42	33.49628161138215	36.0	33.0	40.0	18.0	41.0
43	33.36950976354765	35.0	33.0	40.0	18.0	41.0
44	33.188159648404785	35.0	33.0	40.0	18.0	41.0
45	33.17122575407888	35.0	33.0	40.0	18.0	41.0
46	33.107887656456526	35.0	33.0	40.0	18.0	41.0
47	33.01852443606238	35.0	32.0	40.0	18.0	41.0
48	32.89235443770879	35.0	32.0	40.0	17.0	41.0
49	32.79801262698834	35.0	32.0	40.0	15.0	41.0
50	32.63201094333346	35.0	32.0	39.0	15.0	41.0
51	32.256889843345604	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	6.0
14	25.0
15	63.0
16	181.0
17	497.0
18	1270.0
19	2684.0
20	4881.0
21	7794.0
22	12178.0
23	18447.0
24	28365.0
25	43464.0
26	58271.0
27	61634.0
28	59420.0
29	60501.0
30	66473.0
31	76996.0
32	92920.0
33	118891.0
34	201805.0
35	326156.0
36	162438.0
37	189229.0
38	276802.0
39	491228.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.979990832588076	24.19925873475325	29.5609249859378	18.259825446720875
2	35.2517038646609	24.214749375401286	28.69222170915941	11.841325050778405
3	26.489249453066112	23.74744838251894	36.458407841480806	13.304894322934146
4	23.549329014012258	25.77828830113805	36.83339985279659	13.838982832053098
5	21.02566663718084	30.552283663257175	35.286748264815465	13.135301434746523
6	19.26671708884306	38.44726219856208	32.39008311194274	9.89593760065213
7	82.62419917408644	3.3373442524009436	12.45151239018469	1.5869441833279228
8	83.94204638134826	2.344885147057392	11.962541430057007	1.7505270415373488
9	80.01334903841637	4.084873474055504	13.5604898936267	2.341287593901428
10	45.43260999942016	23.15143963496263	20.335571293557376	11.080379072059836
11	36.64145981088721	20.58418337871188	28.19626726349368	14.578089546907226
12	33.93889323181744	20.395840889958468	30.581191060969214	15.08407481725488
13	21.61778156249775	33.61405534391127	30.502425808930987	14.265737284659991
14	15.80730997406799	37.206064035599695	32.21155982709736	14.775066163234953
15	14.363590730502215	25.264219117228176	46.22411401788703	14.148076134382578
16	16.861012131372483	21.757620569877815	45.145186664336016	16.236180634413685
17	17.17323742115539	22.225556425081802	32.27326844476084	28.327937709001965
18	20.5516361036891	25.21254132424662	36.42882325729235	17.806999314771936
19	24.434623359462858	28.033065322677242	29.514114470755494	18.018196847104413
20	25.209959550805284	25.932559845296748	30.112239426050603	18.74524117784737
21	19.578984702780737	28.52013169584	33.2660661433426	18.63481745803666
22	19.86623874124578	23.9487843867886	32.58485887222211	23.600117999743517
23	16.581376440767134	29.96537460897771	31.566158790917402	21.887090159337745
24	18.452865916654428	25.83838860092004	38.91735658565965	16.791388896765884
25	16.92551414325118	26.713228880140953	36.38658375082585	19.974673225782013
26	16.03784964513312	33.06540732554936	31.164968127795245	19.73177490152227
27	16.58975662341279	31.90851887890086	33.91802742351285	17.583697074173504
28	14.950796053865107	28.357606941499974	39.57587810982018	17.11571889481474
29	15.616555008492341	25.881728535410716	38.708105964446865	19.793610491650078
30	17.364119359195367	29.54839703612409	35.04380761640095	18.043675988279595
31	24.22122497108202	26.748357928605078	30.45866263289256	18.571754467420348
32	23.487112506491467	28.282947132475027	30.535650270430185	17.694290090603317
33	22.517466120572205	28.53545303986893	29.678967053608197	19.268113785950668
34	16.7224428486357	29.518304562078317	32.286812174289174	21.4724404149968
35	16.834432562173124	28.448476901804153	33.33920228279561	21.377888253227113
36	24.178562223067765	27.091056609826737	30.148638199158	18.581742967947495
37	17.44144558997121	32.21638478074183	31.699945444164495	18.642224185122465
38	18.14813200226688	32.70903794233504	27.881375551959884	21.261454503438205
39	17.902524932101475	31.738883666558458	29.466457472477657	20.892133928862407
40	20.78234507137334	28.290184562941732	28.999791341916954	21.927679023767976
41	16.671696187059222	26.54515966152527	30.378627656211048	26.40451649520446
42	19.89899763704244	28.266144443028935	28.01558544675473	23.819272473173893
43	20.025716156441927	28.738016444627096	28.731964090494124	22.504303308436853
44	18.671808769310925	32.06820791487089	29.215009668953158	20.044973646865028
45	16.876714392794398	35.312015785216765	26.105877259104666	21.70539256288417
46	20.854380782802174	32.22832019238868	27.85267977502172	19.064619249787427
47	19.77249073841683	29.043808462884048	29.13404356086658	22.049657237832545
48	20.721694557579262	27.51518696483752	31.127849844056655	20.63526863352657
49	19.2270593558179	26.51159660678786	31.426065838608686	22.83527819878555
50	17.412580516413943	31.492345464987974	29.701017938246522	21.39405608035156
51	17.25763178577883	32.80185481375891	27.465159813892225	22.475353586570037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1695.0
1	2317.5
2	2940.0
3	32856.0
4	62772.0
5	44127.0
6	25482.0
7	23931.5
8	22381.0
9	24010.5
10	25640.0
11	26743.0
12	27846.0
13	27726.0
14	27606.0
15	26106.5
16	24607.0
17	22598.5
18	20590.0
19	19088.0
20	17586.0
21	16724.5
22	15863.0
23	16222.5
24	16582.0
25	16596.5
26	19395.0
27	22179.0
28	24468.0
29	26757.0
30	30621.5
31	34486.0
32	41010.0
33	47534.0
34	53487.5
35	59441.0
36	65521.0
37	71601.0
38	81802.5
39	92004.0
40	123580.5
41	155157.0
42	183145.5
43	211134.0
44	226870.5
45	242607.0
46	239995.0
47	237383.0
48	218946.0
49	200509.0
50	184861.5
51	169214.0
52	151168.5
53	133123.0
54	116319.5
55	99516.0
56	89252.0
57	78988.0
58	69250.5
59	59513.0
60	51325.0
61	43137.0
62	35794.0
63	28451.0
64	23973.5
65	19496.0
66	14349.0
67	9202.0
68	7402.0
69	5602.0
70	4490.5
71	3379.0
72	2623.5
73	1868.0
74	1421.0
75	766.0
76	558.0
77	414.5
78	271.0
79	216.0
80	161.0
81	132.0
82	103.0
83	86.5
84	70.0
85	55.5
86	41.0
87	37.0
88	33.0
89	22.0
90	11.0
91	9.0
92	7.0
93	6.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2362717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.792214814279816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.74102590663072	22.67128532600484
2	8.050950467120927	4.636093906169443
3	3.124019236916085	2.698422988596913
4	1.7244640609402488	1.9860455872838787
5	1.1812300491004815	1.7005114659391678
6	0.8194846305370623	1.4156866511654298
7	0.6247061169646717	1.259067090080901
8	0.47700115709451396	1.0987135825380225
9	0.394258152068383	1.0216408865970492
>10	3.5697870637113778	22.161876108840033
>50	0.8421498890139113	17.39976178650863
>100	0.44616902530561175	18.462331714090407
>500	0.003417113303365518	0.6678288240943229
>1k	0.001039991005372114	0.62359778360537
>5k	1.4857014362458772E-4	0.23385986301073936
>10k+	1.4857014362458772E-4	1.9632764354748293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45896	1.9425094076015028	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5467	0.23138615416065486	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4450	0.18834248875341397	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT	2584	0.10936561594130825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.232415477604808E-5	0.0	0.0	0.04253577554992832	0.0
2	4.232415477604808E-5	0.0	0.0	0.16688414228195758	0.0
3	4.232415477604808E-5	0.0	0.0	0.3049032110066504	0.0
4	4.232415477604808E-5	0.0	0.0	0.45803200298639235	0.0
5	4.232415477604808E-5	0.0	0.0	0.8877491464276086	0.0
6	4.232415477604808E-5	0.0	0.0	1.5550317706267827	0.0
7	4.232415477604808E-5	0.0	0.0	2.0307552703095633	0.0
8	4.232415477604808E-5	0.0	0.0	2.895776345622434	0.0
9	4.232415477604808E-5	0.0	0.0	3.3634582558977653	0.0
10	8.464830955209616E-5	0.0	0.0	3.9853270620222396	0.0
11	8.464830955209616E-5	0.0	0.0	4.519076977902982	0.0
12	8.464830955209616E-5	0.0	0.0	4.940159993769885	0.0
13	8.464830955209616E-5	0.0	0.0	5.149114345899234	0.0
14	8.464830955209616E-5	0.0	0.0	5.245528770479071	0.0
15	8.464830955209616E-5	0.0	0.0	5.345963989762634	0.0
16	8.464830955209616E-5	0.0	0.0	5.572906107671804	0.0
17	8.464830955209616E-5	0.0	0.0	5.846193175060746	0.0
18	8.464830955209616E-5	0.0	0.0	6.2032820689062635	0.0
19	8.464830955209616E-5	0.0	0.0	6.386503334931776	0.0
20	1.2697246432814426E-4	0.0	0.0	6.57687738311444	0.0
21	1.2697246432814426E-4	0.0	0.0	6.824177419470889	0.0
22	1.2697246432814426E-4	0.0	0.0	7.100554150158483	0.0
23	1.2697246432814426E-4	0.0	0.0	7.4193396839316765	0.0
24	1.2697246432814426E-4	0.0	0.0	7.6432767868517475	0.0
25	1.2697246432814426E-4	0.0	0.0	7.844782087740512	0.0
26	1.2697246432814426E-4	0.0	0.0	8.044086532580923	0.0
27	1.2697246432814426E-4	0.0	0.0	8.247581068744163	0.0
28	1.2697246432814426E-4	0.0	0.0	8.47549664221318	0.0
29	1.2697246432814426E-4	0.0	0.0	8.712681205578154	0.0
30	1.2697246432814426E-4	0.0	0.0	9.015129615607794	0.0
31	1.2697246432814426E-4	0.0	0.0	9.273857173753775	0.0
32	1.6929661910419232E-4	0.0	0.0	9.519633540538287	0.0
33	1.6929661910419232E-4	0.0	0.0	9.781154492899487	0.0
34	1.6929661910419232E-4	0.0	0.0	10.036369146199059	0.0
35	1.6929661910419232E-4	0.0	0.0	10.330733642666473	0.0
36	1.6929661910419232E-4	0.0	0.0	10.592127622563346	0.0
37	1.6929661910419232E-4	0.0	0.0	10.869816402048997	0.0
38	1.6929661910419232E-4	0.0	0.0	11.180644994724295	0.0
39	1.6929661910419232E-4	0.0	0.0	11.592712965623898	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	35	1.2126657E-7	45.0	15
CGGCCTA	20	7.034801E-4	45.0	27
CGTTTTT	20515	0.0	44.13356	1
CGACGGT	545	0.0	43.348625	28
GACGGGT	235	0.0	42.12766	4
TTGTACG	130	0.0	41.538464	1
CGCATCG	110	0.0	40.909092	21
ACGGGTA	220	0.0	40.909092	5
TATAGCG	160	0.0	40.78125	1
TAGGGTA	1270	0.0	40.74803	5
CGTTCGG	105	0.0	40.714287	45
TACGGGA	880	0.0	40.653408	4
CGAAAGG	350	0.0	40.500004	2
CACGACG	585	0.0	40.0	26
AATGCGG	315	0.0	40.0	2
GTAGCGA	180	0.0	40.0	34
ATACGAA	395	0.0	39.873417	19
ATTAGCG	175	0.0	39.857143	1
TCTAGCG	85	0.0	39.705883	1
CGGTCTA	595	0.0	39.705883	31
>>END_MODULE