FastQCFastQC Report
Thu 26 May 2016
SRR1548057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548057_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1689816
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79040.467743233582828No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59930.3546539978317166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC351.2123746E-745.00000418
TATGCGT207.034229E-445.00000415
CCGAACG207.034229E-445.00000437
TATCGTG351.2123746E-745.0000041
GTGCGAC207.034229E-445.00000411
AATCGAC207.034229E-445.00000416
CCAATCG502.1827873E-1145.024
TATAGCG900.045.01
ATCGGCA253.8915878E-545.033
CGTACAA302.1661108E-644.99999637
ACGCGAG800.042.1875041
CTTAACG800.042.1875041
CGTTTTT54300.041.809391
GTACGGG6300.041.4285743
AATCGGT556.184564E-1140.90909226
CGACGGT1650.040.9090928
ACGTAGG2100.040.7142872
CGTTAGG2050.040.6097562
TGTTACG1000.040.51
ATTCGAT950.040.26315714