##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548057_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1689816
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.493559062051727	31.0	31.0	33.0	30.0	34.0
2	31.88691253959011	31.0	31.0	34.0	30.0	34.0
3	31.912238373882126	33.0	31.0	34.0	30.0	34.0
4	35.654049908392395	37.0	35.0	37.0	33.0	37.0
5	35.579503922320534	37.0	35.0	37.0	33.0	37.0
6	35.653462862228785	37.0	35.0	37.0	33.0	37.0
7	36.04018425674748	37.0	35.0	37.0	35.0	37.0
8	36.070131304236675	37.0	35.0	37.0	35.0	37.0
9	37.88072665899719	39.0	38.0	39.0	35.0	39.0
10	37.27057442940533	39.0	37.0	39.0	34.0	39.0
11	36.914646920138054	39.0	37.0	39.0	33.0	39.0
12	35.985411429410064	37.0	35.0	39.0	32.0	39.0
13	35.60560558072595	37.0	35.0	39.0	31.0	39.0
14	36.57241853550919	38.0	35.0	41.0	31.0	41.0
15	36.8673234245622	38.0	35.0	41.0	32.0	41.0
16	37.03854975926373	38.0	35.0	41.0	32.0	41.0
17	36.99398987818792	38.0	35.0	41.0	32.0	41.0
18	36.90877231603914	38.0	35.0	41.0	32.0	41.0
19	36.81649954787977	38.0	35.0	40.0	32.0	41.0
20	36.67042683937186	38.0	35.0	40.0	32.0	41.0
21	36.51510519488512	38.0	35.0	40.0	31.0	41.0
22	36.402214797350716	38.0	35.0	40.0	31.0	41.0
23	36.3496670643431	37.0	35.0	40.0	31.0	41.0
24	36.367310997173654	37.0	35.0	40.0	31.0	41.0
25	36.34381139721721	37.0	35.0	40.0	31.0	41.0
26	36.20762556396673	37.0	35.0	40.0	31.0	41.0
27	36.13735459955404	37.0	35.0	40.0	31.0	41.0
28	36.111142869992946	37.0	35.0	40.0	31.0	41.0
29	36.085853134305744	37.0	35.0	40.0	31.0	41.0
30	36.04329642990716	37.0	35.0	40.0	31.0	41.0
31	35.91360006059831	37.0	35.0	40.0	30.0	41.0
32	35.73387635103467	37.0	34.0	40.0	30.0	41.0
33	35.62262163454483	37.0	34.0	40.0	30.0	41.0
34	35.49733166214546	37.0	34.0	40.0	29.0	41.0
35	35.353828464164145	37.0	34.0	40.0	29.0	41.0
36	35.16118914722076	37.0	34.0	40.0	27.0	41.0
37	35.020332982999335	36.0	34.0	40.0	27.0	41.0
38	34.957213684803556	36.0	34.0	40.0	27.0	41.0
39	34.96877707395361	36.0	34.0	40.0	27.0	41.0
40	34.80431242218088	36.0	34.0	40.0	26.0	41.0
41	34.81016453862432	36.0	34.0	40.0	26.0	41.0
42	34.75910690868118	36.0	34.0	40.0	26.0	41.0
43	34.6261811936921	36.0	34.0	40.0	26.0	41.0
44	34.467921359485295	35.0	34.0	40.0	25.0	41.0
45	34.4575107585678	35.0	34.0	40.0	25.0	41.0
46	34.38896838472354	35.0	34.0	40.0	24.0	41.0
47	34.33712368683928	35.0	33.0	40.0	24.0	41.0
48	34.235755253826454	35.0	33.0	40.0	24.0	41.0
49	34.170387190084604	36.0	33.0	40.0	24.0	41.0
50	33.971763789667044	35.0	33.0	40.0	24.0	41.0
51	33.583536905793295	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	9.0
13	9.0
14	15.0
15	47.0
16	119.0
17	259.0
18	679.0
19	1396.0
20	2392.0
21	3798.0
22	6238.0
23	9358.0
24	13573.0
25	19644.0
26	25449.0
27	29431.0
28	31568.0
29	35815.0
30	42583.0
31	52375.0
32	66004.0
33	87672.0
34	163771.0
35	269532.0
36	106899.0
37	135267.0
38	208656.0
39	377182.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.50028405459529	24.434376287122387	29.724952302499208	13.340387355783115
2	30.560013634620574	26.05301405596822	29.91574230567115	13.471230003740054
3	28.551925180019598	24.602678634833616	32.49631912586933	14.349077059277459
4	25.746175915010866	27.976004488062607	31.463188891571626	14.814630705354903
5	23.279753535296148	32.70373815847406	30.067001377664788	13.949506928565004
6	21.714612715230533	40.59146084544116	26.87694991644061	10.816976522887698
7	88.0278681229199	4.224128544172856	6.200201678762658	1.5478016541445931
8	89.47406108120649	2.8786566111339935	5.8995772320773385	1.7477050755821937
9	85.1040586667424	4.977642536228796	7.459155316318464	2.459143480710326
10	50.48484568734111	25.085630624872767	13.96619513603848	10.463328551747646
11	44.456497038730845	20.463648113167352	21.341435990664074	13.738418857437734
12	40.17466990488906	20.48228919598347	24.76695687577819	14.576084023349287
13	22.563995133197935	38.41601689177993	24.17132989627273	14.848658078749402
14	15.667327093600726	41.16075359684132	27.615551042243652	15.55636826731431
15	14.334519261268683	25.144157707111308	46.084307403883024	14.437015627736985
16	16.360361128075482	20.402931443423427	45.07620948079554	18.160497947705547
17	17.427045311442193	21.35599378867285	28.057670184209403	33.15929071567555
18	22.03482509338295	24.916914030876736	33.20728410667197	19.840976769068348
19	27.540631642735068	26.35565055603687	26.503003877345225	19.600713923882836
20	29.645417015817106	23.947281834235206	26.334228105308505	20.07307304463918
21	22.36172459013289	28.047136492967283	29.415036903426174	20.176102013473656
22	22.87917737789203	24.4415368300454	27.041642403669986	25.637643388392583
23	18.906851396838473	30.19044677053596	26.65698513921042	24.24571669341514
24	20.38671666027544	24.693576105327445	37.06817783711362	17.851529397283493
25	17.90236333423284	26.017921477841377	34.38930629133586	21.690408896589926
26	17.50391758629342	33.69065034299592	28.252425116107315	20.553006954603344
27	18.739377541696847	32.97116372433449	29.519190255033685	18.770268478934984
28	15.947535116249343	28.85041921724022	37.726474361705655	17.47557130480478
29	16.450962708365882	24.569657288130777	37.337260388113265	21.64211961539008
30	18.531603440847995	29.42929881123152	32.85150572606722	19.187592021853266
31	27.48056593143869	26.97571806634568	25.687175408446837	19.85654059376879
32	27.110111396743786	27.700353174546816	27.656975670723916	17.532559757985485
33	25.653917349581256	29.535819284466474	25.818787370932693	18.991475995019577
34	19.718123156604033	28.611103220705687	29.182230491367108	22.488543131323173
35	19.7195434295805	27.656798136601857	30.70778120221373	21.915877231603915
36	29.062039890733665	24.824832999569185	27.450917733054958	18.662209376642192
37	20.756461058482106	30.992368399873122	30.143104337987094	18.10806620365768
38	20.368667357866183	31.552606911048304	25.262750500646224	22.81597523043929
39	20.454061270576204	30.41851893934014	28.700639596263734	20.42678019381992
40	22.903144484369896	26.81818612203932	26.81812694399864	23.460542449592143
41	17.936745775871458	25.38808959081936	28.993215829415746	27.68194880389344
42	23.554458000160963	26.08869841450193	25.755348511317212	24.601495074019894
43	23.373491551742912	27.947599028533283	25.3998068428752	23.279102576848604
44	19.68427331733159	31.191502506781806	27.853150875598292	21.271073300288315
45	18.267432667225307	35.8233677512818	23.484154487825894	22.425045093667002
46	22.662704105062325	32.190960435929114	25.82150956080425	19.324825898204303
47	21.989672248339463	27.723610144536448	27.93061493085638	22.35610267626771
48	23.8214101416959	24.818796839419203	29.705068480828682	21.654724538056215
49	22.18407211199326	23.673109971736565	30.295073546468963	23.84774436980121
50	19.335833013771914	30.918336670974828	28.164486547647794	21.581343767605468
51	18.729317274780215	33.00891931429221	24.16801592599431	24.09374748493327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	801.0
1	1486.0
2	2171.0
3	7806.0
4	13441.0
5	10303.5
6	7166.0
7	7025.5
8	6885.0
9	7003.0
10	7121.0
11	7337.0
12	7553.0
13	7324.5
14	7096.0
15	6775.5
16	6455.0
17	6182.0
18	5909.0
19	5894.5
20	5880.0
21	6261.5
22	6643.0
23	6715.0
24	6787.0
25	8455.5
26	12480.5
27	14837.0
28	17745.0
29	20653.0
30	23331.5
31	26010.0
32	32261.0
33	38512.0
34	40415.5
35	42319.0
36	47136.0
37	51953.0
38	62798.5
39	73644.0
40	95555.5
41	117467.0
42	141422.0
43	165377.0
44	166885.0
45	168393.0
46	162303.5
47	156214.0
48	148808.0
49	141402.0
50	139376.5
51	137351.0
52	128064.0
53	118777.0
54	102837.5
55	86898.0
56	76088.0
57	65278.0
58	58419.5
59	51561.0
60	46080.5
61	40600.0
62	35289.5
63	29979.0
64	24194.0
65	18409.0
66	14722.5
67	11036.0
68	9083.5
69	7131.0
70	5794.5
71	4458.0
72	3884.5
73	3311.0
74	2552.0
75	1377.0
76	961.0
77	819.5
78	678.0
79	500.5
80	323.0
81	266.0
82	209.0
83	145.5
84	82.0
85	73.0
86	64.0
87	49.5
88	35.0
89	32.0
90	29.0
91	24.0
92	19.0
93	15.0
94	11.0
95	10.5
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1689816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.94333316850186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48633786889383	19.271112031075326
2	7.941318126463459	3.802832513979551
3	2.5560965339993142	1.8360441277319524
4	1.276213273312805	1.2222719838797125
5	0.8024892881991412	0.9607134195752971
6	0.5623049286373997	0.8078072549193552
7	0.4572463034318086	0.7663600408193582
8	0.36516636446126605	0.6994639940981334
9	0.2907358698729867	0.6265067216762811
>10	3.0343595586446495	16.691712781713708
>50	1.3821891903744707	24.5248027361726
>100	0.8430674516335899	27.667882959138833
>500	0.0019801936602582494	0.29887614687507263
>1k	0.0	0.0
>5k	4.950484150645624E-4	0.8236132883448113
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7904	0.467743233582828	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5993	0.3546539978317166	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9178040686086534E-5	0.0	0.0	0.04213476496849361	0.0
2	5.9178040686086534E-5	0.0	0.0	0.14516373380297026	0.0
3	5.9178040686086534E-5	0.0	0.0	0.22120751608459147	0.0
4	5.9178040686086534E-5	0.0	0.0	0.3370781197479489	0.0
5	5.9178040686086534E-5	0.0	0.0	0.5998286203941731	0.0
6	5.9178040686086534E-5	0.0	0.0	0.9124662093387682	0.0
7	5.9178040686086534E-5	0.0	0.0	1.097042518238672	0.0
8	5.9178040686086534E-5	0.0	0.0	1.6415988486320403	0.0
9	5.9178040686086534E-5	0.0	0.0	1.858841435990664	0.0
10	5.9178040686086534E-5	0.0	0.0	2.2716674478168035	0.0
11	5.9178040686086534E-5	0.0	0.0	2.676741136313066	0.0
12	5.9178040686086534E-5	0.0	0.0	3.0129315854507235	0.0
13	5.9178040686086534E-5	0.0	0.0	3.161586823654173	0.0
14	5.9178040686086534E-5	0.0	0.0	3.213781855539301	0.0
15	5.9178040686086534E-5	0.0	0.0	3.299826726696871	0.0
16	5.9178040686086534E-5	0.0	0.0	3.520560818455974	0.0
17	5.9178040686086534E-5	0.0	0.0	3.7878680282350268	0.0
18	5.9178040686086534E-5	0.0	0.0	4.130153815563351	0.0
19	5.9178040686086534E-5	0.0	0.0	4.302125201797119	0.0
20	5.9178040686086534E-5	0.0	0.0	4.470605083630407	0.0
21	5.9178040686086534E-5	0.0	0.0	4.724538056214405	0.0
22	5.9178040686086534E-5	0.0	0.0	4.986164174087593	0.0
23	5.9178040686086534E-5	0.0	0.0	5.318271338417911	0.0
24	5.9178040686086534E-5	0.0	0.0	5.528590095016262	0.0
25	5.9178040686086534E-5	0.0	0.0	5.725712148541616	0.0
26	5.9178040686086534E-5	0.0	0.0	5.910584347644951	0.0
27	5.9178040686086534E-5	0.0	0.0	6.091550796063003	0.0
28	5.9178040686086534E-5	0.0	0.0	6.303112291515762	0.0
29	5.9178040686086534E-5	0.0	0.0	6.525503368414076	0.0
30	5.9178040686086534E-5	0.0	0.0	6.78174428458483	0.0
31	5.9178040686086534E-5	0.0	0.0	7.029700275059533	0.0
32	5.9178040686086534E-5	0.0	0.0	7.254340117503918	0.0
33	5.9178040686086534E-5	0.0	0.0	7.499869808310491	0.0
34	5.9178040686086534E-5	0.0	0.0	7.723089377778409	0.0
35	5.9178040686086534E-5	0.0	0.0	7.999865074067236	0.0
36	5.9178040686086534E-5	0.0	0.0	8.249833117925265	0.0
37	5.9178040686086534E-5	0.0	0.0	8.50577814389259	0.0
38	5.9178040686086534E-5	0.0	0.0	8.757876597215319	0.0
39	5.9178040686086534E-5	0.0	0.0	9.017431483664494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTC	35	1.2123746E-7	45.000004	18
TATGCGT	20	7.034229E-4	45.000004	15
CCGAACG	20	7.034229E-4	45.000004	37
TATCGTG	35	1.2123746E-7	45.000004	1
GTGCGAC	20	7.034229E-4	45.000004	11
AATCGAC	20	7.034229E-4	45.000004	16
CCAATCG	50	2.1827873E-11	45.0	24
TATAGCG	90	0.0	45.0	1
ATCGGCA	25	3.8915878E-5	45.0	33
CGTACAA	30	2.1661108E-6	44.999996	37
ACGCGAG	80	0.0	42.187504	1
CTTAACG	80	0.0	42.187504	1
CGTTTTT	5430	0.0	41.80939	1
GTACGGG	630	0.0	41.428574	3
AATCGGT	55	6.184564E-11	40.909092	26
CGACGGT	165	0.0	40.90909	28
ACGTAGG	210	0.0	40.714287	2
CGTTAGG	205	0.0	40.609756	2
TGTTACG	100	0.0	40.5	1
ATTCGAT	95	0.0	40.263157	14
>>END_MODULE