FastQCFastQC Report
Thu 26 May 2016
SRR1548055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548055_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1707869
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50570.2960999936177775No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44950.26319348849355545No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17920.10492608039609594No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.03425E-445.00000422
GCGTAGC207.03425E-445.00000437
TATTACG502.1827873E-1145.01
CGCAATA253.891605E-545.043
CGACGGT3200.042.8906328
CGCATCG700.041.78571321
GGCGTAG1350.041.6666641
ACGACGG3300.041.5909127
CGGTCTA3250.041.53846431
ACGTAAG650.041.538461
CACGACG3300.040.90909226
AATACGG1550.040.6451642
CTAACCG501.0822987E-940.540
CGTAAGG2500.040.4999962
TCACGAC3400.040.3676525
CGTTTTT31000.039.9193531
GGCGATT5450.039.2201848
AGGGCGA9200.039.1304326
AACACGT1100.038.86363641
GGGCGAT16150.038.591337
Produced by FastQC (version 0.11.5)