FastQCFastQC Report
Thu 26 May 2016
SRR1548054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548054_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1564158
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC88310.5645849076627809No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.2759312038809379No Hit
AGCTGAGGGAAATGTATTTTGAAAACATTTCACACTTCAACTTTGTATCCA16270.10401762481795317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.0340675E-445.00000436
ACTATCG406.8175723E-945.00000416
ACACGGC207.0340675E-445.00000436
CATATTA207.0340675E-445.00000432
CGTATCA207.0340675E-445.00000440
TGGCGTA207.0340675E-445.0000041
CGTTCCC207.0340675E-445.00000425
CTAAACG406.8175723E-945.00000445
ACGGACG207.0340675E-445.00000439
ACAGCGT207.0340675E-445.00000430
CTCGAAG207.0340675E-445.00000442
CCGGTTG207.0340675E-445.0000041
AACTCGT207.0340675E-445.00000429
CACAATA207.0340675E-445.00000433
CGCGTGA207.0340675E-445.0000044
ATACGCG207.0340675E-445.0000041
TTCGCTA207.0340675E-445.00000414
TACGTCA207.0340675E-445.00000417
CGAAGTC207.0340675E-445.00000431
TATCCGA207.0340675E-445.00000424